0
Why was it important to stop the gel electrophoresis before the dye ran off the edge of the gel?
Wiki User
∙ 10y ago
You would lose your DNA.
Wiki User
∙ 10y ago
Wiki User
∙ 11y agoDAFUQ?!
Add your answer:
Earn +20 pts
What is the purpose of a stain in electrophoresis?
I think the Question should be " What is the use of a dye in electrophoresis?" to be more specific, The normal dyes that are incorporated into the sample loading buffer in case of agarose gel is bromophenol blue and xylene cyanol.These are used to track the movement of the sample, so that we can stop the electrophoresis when the dye front is nearly 80%(can vary depending on the molecular weight of the sample) away from the well. In case of polyacrylamide gel electrophoresis the dye has many functions: gives density to the sample so that it sits properly in the well. SDS binds to the amino acid and makes it anionic, so that the movement of the sample is based on molecular weight, if not the charge of the protein will also influence the movement. it also helps us to track the movement of the sample. DTT or mercaptoethanol helps in the breaking of cysteine bonds.. That's it.. But the question is too broad and can be specific!!!!!!!!!1
Why water is important to cells Water?
stop being lazy and answer it youself
How long before bed time should teens stop playing video games?
teens between 13 and 15: should stop playing videogames atleast 30 to 45 minutes before going to bed.
How do you stop a chestnut from exploding when it's heated?
Before heating it, cut a small "X" into the shell.
Why is it important for the release of acetylcholine to stop and for enzymes to destroy any remaining acetylcholine in the neuromuscular junction?
it has caca
How do you stop a motorcycle from backfiring?
Rev the engine six or seven times before coming to a complete stop. Rev it all the way to the edge every time you stop.
When a stop is legally required at an intersection name 4 possible stopping areas?
If you are the first vehicle approaching an intersection with a red light or stop sign, stop behind the stop line if it is marked on the pavement. If there is no stop line, stop at the crosswalk, marked or not. If there is no crosswalk, stop at the edge of the sidewalk. If there is no sidewalk, stop at the edge of the intersection.
When at a stop sign and there is no white line where do you stop?
If there is no white line, you must stop before the stop sign, not past it. In some cases, it is okay to edge slowly forward so you are able to look left and right to make sure there is no traffic. If at a 3, 4 or all-way stop sign, stop before the stop sign and if there is no other cars at the other stop signs, you may continue on. Whoever stops first at a 3, 4 or all-way stop has the right away to go first, but many people don't follow that now.
Do speech marks come after the full stop or before?
In British English, the speech marks typically come before the full stop. In American English, they usually come before the closing punctuation mark. It's important to be consistent within the style guide you are following.
How do you stop child hurting themselves on edge of table?
you can put padding around it
A stop sign is consider a stop position?
I recall this from Drivers Ed. Technically a stop sign is not a stop position. You should use the crosswalk, sidewalk or edge of the road as your stop position - not the sign itself.
What is the importance of yellow lane on the platform beside the passageway of trains in train stations?
The yellow line is there to stop passengers getting too close to the platform edge when trains are not scheduled to stop at any given station, they are also there to make sure passengers don't get too close to the train before it comes to a complete stop.
What causes your teeth to be on edge and how can you stop it?
How the teeth are aligned in relation to the jaw is one cause of teeth being on edge. Another cause can be stress. Dental braces is one way to stop it. Another way would be to relax the jaw.
Does your discharge stop before you get your period?
It can stop a few days before. (or at least it does for me)
Can only lawyers can do will?
No, you can do it yourself. However, make sure you educate yourself about wills before attempting it. I would stop at your library and ask the reference librarian for the information you will need. It is not difficult, but extremely important to make sure you do it appropriately.No, you can do it yourself. However, make sure you educate yourself about wills before attempting it. I would stop at your library and ask the reference librarian for the information you will need. It is not difficult, but extremely important to make sure you do it appropriately.No, you can do it yourself. However, make sure you educate yourself about wills before attempting it. I would stop at your library and ask the reference librarian for the information you will need. It is not difficult, but extremely important to make sure you do it appropriately.No, you can do it yourself. However, make sure you educate yourself about wills before attempting it. I would stop at your library and ask the reference librarian for the information you will need. It is not difficult, but extremely important to make sure you do it appropriately.No, you can do it yourself. However, make sure you educate yourself about wills before attempting it. I would stop at your library and ask the reference librarian for the information you will need. It is not difficult, but extremely important to make sure you do it appropriately.No, you can do it yourself. However, make sure you educate yourself about wills before attempting it. I would stop at your library and ask the reference librarian for the information you will need. It is not difficult, but extremely important to make sure you do it appropriately.
When was Blind Before I Stop created?
Blind Before I Stop was created in 1986-09.
How do you do perineal preparation before birth?
You are suppose to do a massage to gently stretch the muscles in that area. Personally I don't think it makes a difference. The most important thing is to stop pushing before the baby is crowning. This allows the area to stretch out gradually. Your doctor should tell you when to stop.
