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How do you get agarose gel to solidify when it doesn't solidify after 10 min in the refrigerator?
Try increasing the concentration of agarose in your gel mixture or extending the cooling time in the refrigerator. You can also check if the agarose powder is expired or if there was an error in the preparation process. If the issue persists, consider using a different brand or batch of agarose.
What happens when you add bromide and zinc chloride solution?
It is not a true chemical reaction.
Major steps in completing gel electrophoresis?
1. There is a permeable gel with a row of holes on one side. 2. Different DNA samples are placed in holes using pipette. 3. An electric current is sent through the gel, with the opposite side as positive (the DNA is negatively charged). 4. Since the larger parts of DNA cannot get far through the gel and the smaller parts can. Several bands will be formed on the gel. 5. The bands are visible under UV light 6. These bands can be used to compare genetic similarity to determine the parent/sibling.
What is the ions and formula for barium and bromine?
Ba+2 Br-1 -----> these are the ions and their chargesBa+2 Br+1 Br+1 ----> the charges must add to zero, so one positive Br ion is added to cancel out the +2 Ba ionBaBr2 ------> simplifyName: Barium bromide
How do you convery propanoic acid to ethanoic acid?
onvert the ethyl bromide into Grignard's reagent, ethyl magnesium bromide then allow to react with dry ice (Solid carbon dioxide) then acidic hydrolysis produces the propionic acid. CH3-Br + Mg --- anhydrous ether---> CH3-CH2-Mg-Br CH3-CH2-Mg-Br + CO2 ----H+/H2O---> CH3-CH2-COOH
What are the steps in electrophoresis?
1) Add loading dye to desired sample(s). 2) Make a gel. Agarose gel, for example, is made by mixing agarose powder with buffer, heating until the powder has dissolved, adding ethidium bromide, pouring the mixture into a gel box, and putting in combs which are pulled out after the gel has cooled to make wells. 3) Make sure the wells are positioned so that the material that is being analyzed is has room to run. For example, since DNA is negative and runs towards to positive electrode, the wells are best off being positioned on the far negative side. 4) Add enough running buffer in the gel box to cover the gel. 5) Load sample(s) (a ladder is usually loaded as well). 6) Attach the electrodes to the power source. 7) Run for the designated amount of time. 8) After the gel has run, turn off the power source, remove the gel carefully and analyze using a UV light box.
How do you get agarose gel to solidify when it doesn't solidify after 10 min in the refrigerator?
Try increasing the concentration of agarose in your gel mixture or extending the cooling time in the refrigerator. You can also check if the agarose powder is expired or if there was an error in the preparation process. If the issue persists, consider using a different brand or batch of agarose.
What happens when you add bromide and zinc chloride solution?
It is not a true chemical reaction.
How do you test for bromide ions?
One common test for bromide ions is the silver nitrate test, where adding silver nitrate to a solution containing bromide ions produces a cream-colored precipitate of silver bromide. Another test is the starch-iodide test, which involves adding starch and iodine solution to the sample, causing a blue color to form in the presence of bromide ions.
If you dissolve potassium bromide in water and add electricity What will happen?
If you dissolve potassium bromide in water and add electricity, the water will undergo electrolysis. Potassium ions will move towards the negative electrode (cathode), while bromide ions move to the positive electrode (anode). This process will lead to the decomposition of water into hydrogen gas and oxygen gas at the respective electrodes.
What is the chemical formula for the compound formed between magnesium and bromine?
2Mg + Br2 ---> 2MgBr Magnesium Bromide
Function of bromophenolblue in agarose gel electrophoresis?
Bromophenol blue or commasive blue functions as a sample staining dye or DNA staining dye it is mixed with sample before loading the sample in wells. The migration of bromophenol blue is same as of DNA i.e. it carries negative charge and move in same direction of DNA with the speed equals to 200-400bp of DNA.It also prevent backflow of sample in vertical gel electrophoresis as the sample is light from the loading buffer which tends to come back from the well so bromophenol blue prevent the back flow.IUPAC NAME:2,6-dibromo-4-[3-(3,5-dibromo-4-hydroxyphenyl)-1,1-dioxo-3-benzooxathiolyl]phenol.Bromphenol blue does not stain DNA. It is simply a dye that 1) helps you visualise your sample as you load it and 2) migrates (unrelated to the DNA) at a speed that is indeed equivalent to about 200-400bp of DNA, depending on the percentage of gel, giving an indication of how far your samples have run. It also does not prevent "backflow". Usually the buffer which you add to your DNA sample before loading on a gel (ie loading buffer) contains a dye such as bromophenol blue (there are others) and will also contain a dense substance, usually glycerol or ficoll. It is the glycerol or ficoll which due to its density will make the sample more dense than the buffer which the gel is run in, and will prevent it floating out of the well.In order to visualise (stain) the DNA you need an agent such as ethidium bromide or sybr green that intercalates with the DNA (slides between the basepairs) and fluoresces under UV light.Coommassie (not commasive) blue is a dye that will stain proteins (not DNA) but is used after the gel has been run to stain the gel. If you use it with an agarose gel, I'm guessing - having never tried it) you would just simply make a big blue mess and not see anything.
What is the chemical formula for copper II bromide?
Cu+2 Br-1
When doing gel electrophoresis why do researchers add a ladder made up of molecules of known size?
to help the researchers identify the size of each molecule after the separation has occurred
How do you design a procedure to test water that you suspect has a high concentration of bromide ion that got dissolved in the water?
add chlorine to displace the bromine and if the water turns brown or reddish-brown or whatever the colour bromine is.. lol it means that there is bromide ions inside the water
What happens when you add sodium hydroxide to copper bromide?
When you combine these substances, a metathesis reaction occurs. In this reaction, copper becomes bonded to hydroxide ions. Because copper hydroxide is insoluble, it precipitates out of solution.
How many atoms are in NH4Br?
Any molecular formula will tell you how many atoms are in it. I don't want to just give you the answer so let's use another atom...C6H12O6. Your basic monosaccharide. It has... 6 carbon atoms 12 hydrogen 6 oxygen Add 'em up and you get 24 atoms. An atom that doesn't have a number behind it has only one example of it in each molecule...CH4 has one carbon and four hydrogens.
