0
Obligate anaerobes do not need or use oxygen as a nutrient. In fact it is a toxic substance to them which either kills or inhibits their growth.
Thioglycollate allows obligate anaerobes to grow because it consists of sodium thioglycollate, thioglycollic acid and L-crystine which reduce oxygen to water. This creates an anaerobic environment allowing obligate anaerobes to grow.
Wiki User
∙ 12y ago
Add your answer:
Earn +20 pts
Where would you expect to see growth of a strict aerobe anaerobe microphile and facultative anaerobe in the fluid thioglycollate medium?
The sodium thioglycollate in the broth creates a redox potential in the tube, with higher levels of oxygen at the top of the tube, and a complete absence of oxygen at the bottom of the tube.Fluid thioglycollate broth also typically contains a redox potential indicator such resazurin, which produces a pink color in an oxidized environment. As with the BHI media, organisms will only be able to grow where their oxygen requirements are met, and will localize to the area(s) of their oxygen requirements in the fluid thioglycollate broth.Obligate aerobes will grown on or very close to the top (high oxygen). Obligate anaerobes will only grow on the bottom (no oxygen). Facultative anaerobes will grow throughout but more on top. Microaerophiles will be found in a band where only a small amount of oxygen is found. They would be close to but not on the top.
What two enzymes are present in obligate aerobes but lacking in obligate anaerobes?
Catalase and superoxide dismutase.
What kind of bacterium cannot tolerate oxygen?
The bacterium that cannot tolerate oxygen is Anaerobes, but there are different kinds and each has different tolerances when it comes to oxygen. Obligate Anaerobes can get killed by oxygen. Facultative anaerobes those can grow without oxygen. Aero-tolerant anaerobes they never use oxygen BUT they can tolerate it. Microaerophiles require i tiny bit of oxygen but anymore than the little bit they need and they cannot grow.
What type of bacteria can live in the presence of oxygen?
only obligate aerobes and facultive anaerobes.
What is reducing media in microbiology?
it is a type of media used for the growth of obligate anaerobes.
Can obligate anaerobes grow on a slant that is incubated anaerobically?
Yes, obligate anaerobes can grow on a slant that is incubated anaerobically by using special anaerobic incubation methods such as gas-packs or anaerobic jars to create an oxygen-free environment. These methods allow obligate anaerobes to grow on the slant without being exposed to oxygen.
Bacteria that only grows with the absence of oxygen?
Bacteria that can ONLY grow in the absence of oxygen are called obligate anaerobes.
What is the different between obligate anaerobe and facultative anaerobe?
Facultative anaerobes does not need o2 to grow but can also grow with o2. Obligate anaerobe cannot grow at all in the presence of o2.
Does b.subtilis facultative anaerobes in thioglycollate broth?
No
How do obligate aerobes and obligate anaerobes differ?
Answerobligate aerobes require oxygen by using cellular respiration or fermentation in order to survive. obligate anaerobes do not require it and will die if they are exposed to oxygen.
Why are cysteine and sodium sulfide added to the growth media?
To culture obligate anaerobes, all molecular oxygen must be removed and kept out of the medium. Addition of oxygen-binding agents such as thioglycollate, the amino acid cysteine, or sodium sulfide to the medium prevents oxygen from killing the anaerobes you want to culture.
Where would you expect to see growth of a strict aerobe anaerobe microphile and facultative anaerobe in the fluid thioglycollate medium?
The sodium thioglycollate in the broth creates a redox potential in the tube, with higher levels of oxygen at the top of the tube, and a complete absence of oxygen at the bottom of the tube.Fluid thioglycollate broth also typically contains a redox potential indicator such resazurin, which produces a pink color in an oxidized environment. As with the BHI media, organisms will only be able to grow where their oxygen requirements are met, and will localize to the area(s) of their oxygen requirements in the fluid thioglycollate broth.Obligate aerobes will grown on or very close to the top (high oxygen). Obligate anaerobes will only grow on the bottom (no oxygen). Facultative anaerobes will grow throughout but more on top. Microaerophiles will be found in a band where only a small amount of oxygen is found. They would be close to but not on the top.
Why can media such as brain-heart infusion and thioglycollate be used for the cultivation of anaerobes?
Brain-heart infusion and thioglycollate media can support the growth of anaerobes because they contain reducing agents that help to create anaerobic conditions by removing oxygen from the medium. Additionally, these media provide the necessary nutrients and environment for anaerobic bacteria to grow and thrive without the presence of oxygen.
Bacteria that are killed by oxygen are called obligate?
anaerobes
What two enzymes are present in obligate aerobes but lacking in obligate anaerobes?
Catalase and superoxide dismutase.
What kind of bacterium cannot tolerate oxygen?
The bacterium that cannot tolerate oxygen is Anaerobes, but there are different kinds and each has different tolerances when it comes to oxygen. Obligate Anaerobes can get killed by oxygen. Facultative anaerobes those can grow without oxygen. Aero-tolerant anaerobes they never use oxygen BUT they can tolerate it. Microaerophiles require i tiny bit of oxygen but anymore than the little bit they need and they cannot grow.
What type of bacteria can live in the presence of oxygen?
only obligate aerobes and facultive anaerobes.
