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What chemical type is thioglycollate broth?
Thioglycollate broth is a reducing medium used primarily for the cultivation of anaerobic bacteria. It contains sodium thioglycollate, which acts as a reducing agent to create a low-oxygen environment, facilitating the growth of anaerobes. The broth also contains nutrients such as peptones and glucose to support microbial growth. Additionally, it is often supplemented with resazurin, a dye that indicates the presence of oxygen by changing color.
Why is there a colored band at the surface of fluid thioglycollate medium which is more desirable a thick or thin colored band?
The higher viscosity of the Fluid Thioglycollate Medium prevents rapid uptake of oxygen and helps maintain the stratification of organisms growing in different layers of the broth.Oxygen is driven out of the broth by autoclaving, but as the broths sit at room temperature, oxygen begins to diffuse back into the tube. Any increase in the oxygen content is indicated by the redox indicator sodium resazurin which changes its color to red.Obligate aerobes will only grow in this oxygen-rich top layer. Obligate anaerobes will only grow in the lower areas of the tube. Microaerophiles will grow in a thin layer below the richly-oxygenated layer. Facultative or aerotolerant anaerobes can grow throughout the medium but will primarily grow in the middle of the tube, between the oxygen-rich and oxygen-free zones.
Can a strict aerobe be distinguished from an anaerobe in thioglycollate broth?
Yes. Thioglycollate is a reducing medium that can establish a gradiation in oxygen content. Oxygen is highest at the top of the tube and absent in the deeper regions.Source: "Foundations in Microbiology" by Kathleen Park Talaro.
How can you tell if thioglycollate medium is prepared correctly?
Thioglycollate medium should be clear and have a slightly amber color. There should be no precipitates or cloudiness. Additionally, performing a sterility test before use can confirm if the medium was prepared correctly.
How does thioglycollate work?
Thioglycolate is a reducing agent that helps to create anaerobic conditions in culture media by removing oxygen. This is achieved by reacting with oxygen and forming sulfur dioxide, which depletes the available oxygen in the medium. In microbiology, thioglycolate is often used to culture anaerobic bacteria that cannot survive in the presence of oxygen.
Why will obligate anaerobes grow in thioglycollate?
Obligate anaerobes do not need or use oxygen as a nutrient. In fact it is a toxic substance to them which either kills or inhibits their growth. Thioglycollate allows obligate anaerobes to grow because it consists of sodium thioglycollate, thioglycollic acid and L-crystine which reduce oxygen to water. This creates an anaerobic environment allowing obligate anaerobes to grow.
What chemical type is thioglycollate broth?
Thioglycollate broth is a reducing medium used primarily for the cultivation of anaerobic bacteria. It contains sodium thioglycollate, which acts as a reducing agent to create a low-oxygen environment, facilitating the growth of anaerobes. The broth also contains nutrients such as peptones and glucose to support microbial growth. Additionally, it is often supplemented with resazurin, a dye that indicates the presence of oxygen by changing color.
Where would you expect to see growth of a strict aerobe anaerobe microphile and facultative anaerobe in the fluid thioglycollate medium?
The sodium thioglycollate in the broth creates a redox potential in the tube, with higher levels of oxygen at the top of the tube, and a complete absence of oxygen at the bottom of the tube.Fluid thioglycollate broth also typically contains a redox potential indicator such resazurin, which produces a pink color in an oxidized environment. As with the BHI media, organisms will only be able to grow where their oxygen requirements are met, and will localize to the area(s) of their oxygen requirements in the fluid thioglycollate broth.Obligate aerobes will grown on or very close to the top (high oxygen). Obligate anaerobes will only grow on the bottom (no oxygen). Facultative anaerobes will grow throughout but more on top. Microaerophiles will be found in a band where only a small amount of oxygen is found. They would be close to but not on the top.
Why is there a colored band at the surface of fluid thioglycollate medium which is more desirable a thick or thin colored band?
The higher viscosity of the Fluid Thioglycollate Medium prevents rapid uptake of oxygen and helps maintain the stratification of organisms growing in different layers of the broth.Oxygen is driven out of the broth by autoclaving, but as the broths sit at room temperature, oxygen begins to diffuse back into the tube. Any increase in the oxygen content is indicated by the redox indicator sodium resazurin which changes its color to red.Obligate aerobes will only grow in this oxygen-rich top layer. Obligate anaerobes will only grow in the lower areas of the tube. Microaerophiles will grow in a thin layer below the richly-oxygenated layer. Facultative or aerotolerant anaerobes can grow throughout the medium but will primarily grow in the middle of the tube, between the oxygen-rich and oxygen-free zones.
Can a strict aerobe be distinguished from an anaerobe in thioglycollate broth?
Yes. Thioglycollate is a reducing medium that can establish a gradiation in oxygen content. Oxygen is highest at the top of the tube and absent in the deeper regions.Source: "Foundations in Microbiology" by Kathleen Park Talaro.
How can you tell if thioglycollate medium is prepared correctly?
Thioglycollate medium should be clear and have a slightly amber color. There should be no precipitates or cloudiness. Additionally, performing a sterility test before use can confirm if the medium was prepared correctly.
How does thioglycollate work?
Thioglycolate is a reducing agent that helps to create anaerobic conditions in culture media by removing oxygen. This is achieved by reacting with oxygen and forming sulfur dioxide, which depletes the available oxygen in the medium. In microbiology, thioglycolate is often used to culture anaerobic bacteria that cannot survive in the presence of oxygen.
How does the thioglycollate model of peritonitis work?
In the thyoglycollate model of peritonitis, researchers inject thyoglycollate broth into the peritoneal cavities of laboratory animals to induce peritonitis. Then they test their materials to see if they are effective in curing peritonitis.
What is thioglycolate?
Thioglycolate (thio) broth contains Sodium thioglycolate, a reducing agent that creates anaerobic conditions when it reduces molecular oxygen to water. Dyes such as resazurin or methylene blue are usually added to the broth to provide a visual indication of the presence of oxygen. Resazurin is pink when oxidized and colorless when reduced. Methylene blue is blue when oxidized and colorless when reduced. The pink band near the top of the broth results when oxygen diffuses in. Strict aerobes will grow only in the pink band, microaerophiles will grow near the bottom of the band where the concentration of oxygen is lower. The absence of pink in the rest of the tube indicates the absence of oxygen and a suitable environment for strict anaerobes. Both facultative anaerobes and aerotolerant anaerobes will grow throughout the tube; however, facultative anaerobes will grow most densely where oxygen is present. Other constituents:L-Cysteine Yeast Extract Glucose Agar(may be or not) Peptone Distilled Water. *The use of thioglycolate broth permits growth of anaerobic bacteria. In addition, growth patterns can help distinguish aerotolerance of bacteria. E.g., Clostridium sporogenes, Clostridium perfringens. PROCEDURE Step 1. Label 2 Blood Agar plates, one plate will be titled "Aerobic" and the other titled"Anaerobic". Step 2. Using your inoculating loop, streak each plate with Clostridium perfringens. Step 3. Place the anaerobic Blood Agar plate in a Bio-Bag or Gas Pac Jar. The instructor will demonstrate proper set up of the environmental chamber. Step 4. Incubate both plates at 37C for 24-48 hours. Step 5. Obtain Three Thioglycolate Broth tubes -DO NOT SHAKE THESE TUBES!. Gently label each Thioglycolate Broth with one of the assigned species being careful not to tip the tube horizontally . Step 6. Using your inoculating loop, inoculate each Thioglycolate Broth with it's assigned species. Step 7. Incubate the Thioglycolate Broth at 37C for 24-48 hours. Note: Procedure taken fromhttp://bioweb.wku.edu/courses/Biol208/Lab_Manual/208%20week%2011.pdf.Dr.Ank
What is thioglycolate broth?
Thioglycolate (thio) broth contains Sodium thioglycolate, a reducing agent that creates anaerobic conditions when it reduces molecular oxygen to water. Dyes such as resazurin or methylene blue are usually added to the broth to provide a visual indication of the presence of oxygen. Resazurin is pink when oxidized and colorless when reduced. Methylene blue is blue when oxidized and colorless when reduced. The pink band near the top of the broth results when oxygen diffuses in. Strict aerobes will grow only in the pink band, microaerophiles will grow near the bottom of the band where the concentration of oxygen is lower. The absence of pink in the rest of the tube indicates the absence of oxygen and a suitable environment for strict anaerobes. Both facultative anaerobes and aerotolerant anaerobes will grow throughout the tube; however, facultative anaerobes will grow most densely where oxygen is present. Other constituents:L-Cysteine Yeast Extract Glucose Agar(may be or not) Peptone Distilled Water. *The use of thioglycolate broth permits growth of anaerobic bacteria. In addition, growth patterns can help distinguish aerotolerance of bacteria. E.g., Clostridium sporogenes, Clostridium perfringens. PROCEDURE Step 1. Label 2 Blood Agar plates, one plate will be titled "Aerobic" and the other titled"Anaerobic". Step 2. Using your inoculating loop, streak each plate with Clostridium perfringens. Step 3. Place the anaerobic Blood Agar plate in a Bio-Bag or Gas Pac Jar. The instructor will demonstrate proper set up of the environmental chamber. Step 4. Incubate both plates at 37C for 24-48 hours. Step 5. Obtain Three Thioglycolate Broth tubes -DO NOT SHAKE THESE TUBES!. Gently label each Thioglycolate Broth with one of the assigned species being careful not to tip the tube horizontally . Step 6. Using your inoculating loop, inoculate each Thioglycolate Broth with it's assigned species. Step 7. Incubate the Thioglycolate Broth at 37C for 24-48 hours. Note: Procedure taken fromhttp://bioweb.wku.edu/courses/Biol208/Lab_Manual/208%20week%2011.pdf.Dr.Ank
What alternatives can you use instead of chicken broth in recipes?
Vegetable broth, beef broth, mushroom broth, or water with added seasonings like soy sauce or miso can be used as alternatives to chicken broth in recipes.
Can chicken broth cause salmonella?
Yes, if the chicken used in the broth has salmonella, then it is possible that the broth will have it as well.
