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Why is there a colored band at the surface of fluid thioglycollate medium which is more desirable a thick or thin colored band?
Wiki User
∙ 8y ago
The higher viscosity of the Fluid Thioglycollate Medium prevents rapid uptake of oxygen and helps maintain the stratification of organisms growing in different layers of the broth.
Oxygen is driven out of the broth by autoclaving, but as the broths sit at room temperature, oxygen begins to diffuse back into the tube. Any increase in the oxygen content is indicated by the redox indicator sodium resazurin which changes its color to red.
Obligate aerobes will only grow in this oxygen-rich top layer. Obligate anaerobes will only grow in the lower areas of the tube. Microaerophiles will grow in a thin layer below the richly-oxygenated layer. Facultative or aerotolerant anaerobes can grow throughout the medium but will primarily grow in the middle of the tube, between the oxygen-rich and oxygen-free zones.
Wiki User
∙ 13y ago
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How can you tell if thioglycollate medium is prepared correctly?
Thioglycollate medium is correctly prepared when a red/brown color appears at the top of the broth. This is beacuse of the Reazurin which is reactive to oxygen.
How does thioglycollate work?
Thioglycollate broth is an enriched medium used to determine the oxygen requirements of microorganisms. The diffusion of oxygen from the top of the broth provides a range of concentrations.
Why is undefined medium desirable?
It is desirable because it is easier to prepare.
Can a strict aerobe be distinguished from an anaerobe in thioglycollate broth?
Yes. Thioglycollate is a reducing medium that can establish a gradiation in oxygen content. Oxygen is highest at the top of the tube and absent in the deeper regions.Source: "Foundations in Microbiology" by Kathleen Park Talaro.
What rock in the igneous family are medium colored and have no silica?
Igneous rocks that are medium colored and have no silica are called mafic rocks. They are mainly composed of magnesium and iron.
The properties and purposes of thioglycollate medium?
A thioglycollate medium is used for culturing anaerobic bacteria.
How can you tell if thioglycollate medium is prepared correctly?
Thioglycollate medium is correctly prepared when a red/brown color appears at the top of the broth. This is beacuse of the Reazurin which is reactive to oxygen.
What is the function of thiogllycollic acid in thioglycollate?
It removes trapped oxygen from the medium
How does thioglycollate work?
Thioglycollate broth is an enriched medium used to determine the oxygen requirements of microorganisms. The diffusion of oxygen from the top of the broth provides a range of concentrations.
Can a strict aerobe be distinguished from an anaerobe in thioglycollate broth?
Yes. Thioglycollate is a reducing medium that can establish a gradiation in oxygen content. Oxygen is highest at the top of the tube and absent in the deeper regions.Source: "Foundations in Microbiology" by Kathleen Park Talaro.
Why is undefined medium desirable?
It is desirable because it is easier to prepare.
Why you use Fluid Thioglycollate medium for bacterial contamination checking?
Fluid Thioglycollate is a medium used to grow and observe all manner of aerotolerance in bacteria. Therefore if you were to place a sample in it to see if it contained bacteria or not, given the correct temperature needed, the bacteria would be able to be observed fairly quickly as they would grow exponentially in this nutrient rich environment.
What rock in the igneous family are medium colored and have no silica?
Igneous rocks that are medium colored and have no silica are called mafic rocks. They are mainly composed of magnesium and iron.
Why use fresh thioglycollate medium?
A fluid thioglycollate is preferably used soon after preparation to minimize the amount of oxygen that is permitted to diffuse towards the bottom of the medium. The longer you wait before using this medium, the more oxygen is allowed to diffuse into it; more oxygen will negatively affect the growth of obligate anaerobes but would positively affect the growth of aerobic organisms.
What is the granules used in vibrating polish machine for sport rim?
Those "granules" are the polishing " medium" used for a desirable polish or surface. Many different mediums are used. Glass beads, Walnut shells, metal slag(waste products from welding processes), plastic beads,etc. What ever is used is based on the material being polished and the surface texture being sought after.Usually the softer the medium and smaller the medium, the shinier finish.
Example of surface wave?
In geology, a seismic wave that travels along the surface of a medium and that has a stronger effect near the surface of the medium than it has in the interior.
The actual material an artist uses such an acrylic paint markers ink charcoal photography and colored pencils?
medium
