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A fluid thioglycollate is preferably used soon after preparation to minimize the amount of oxygen that is permitted to diffuse towards the bottom of the medium. The longer you wait before using this medium, the more oxygen is allowed to diffuse into it; more oxygen will negatively affect the growth of obligate anaerobes but would positively affect the growth of aerobic organisms.
Wiki User
∙ 12y ago
Wiki User
∙ 10y agoI believe the importance of using a fresh Thioglycollate media is because during the autoclave process oxygen is taken out of the media creating that oxygen gradient. This oxygen gradient is needed to examine oxygen rich and poor environments. After autoclaving is done oxygen slowly diffusses back into the media. Older media will not provide the necessary anaerobic environment needed to display growth for obligate anaerobes.
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How can you tell if thioglycollate medium is prepared correctly?
Thioglycollate medium is correctly prepared when a red/brown color appears at the top of the broth. This is beacuse of the Reazurin which is reactive to oxygen.
How does thioglycollate work?
Thioglycollate broth is an enriched medium used to determine the oxygen requirements of microorganisms. The diffusion of oxygen from the top of the broth provides a range of concentrations.
What is the use of resuspension of supernatant in fresh medium?
LB medium
Can a strict aerobe be distinguished from an anaerobe in thioglycollate broth?
Yes. Thioglycollate is a reducing medium that can establish a gradiation in oxygen content. Oxygen is highest at the top of the tube and absent in the deeper regions.Source: "Foundations in Microbiology" by Kathleen Park Talaro.
Does b.subtilis facultative anaerobes in thioglycollate broth?
No
The properties and purposes of thioglycollate medium?
A thioglycollate medium is used for culturing anaerobic bacteria.
How can you tell if thioglycollate medium is prepared correctly?
Thioglycollate medium is correctly prepared when a red/brown color appears at the top of the broth. This is beacuse of the Reazurin which is reactive to oxygen.
What is the function of thiogllycollic acid in thioglycollate?
It removes trapped oxygen from the medium
How does thioglycollate work?
Thioglycollate broth is an enriched medium used to determine the oxygen requirements of microorganisms. The diffusion of oxygen from the top of the broth provides a range of concentrations.
What is the use of resuspension of supernatant in fresh medium?
LB medium
Why you use Fluid Thioglycollate medium for bacterial contamination checking?
Fluid Thioglycollate is a medium used to grow and observe all manner of aerotolerance in bacteria. Therefore if you were to place a sample in it to see if it contained bacteria or not, given the correct temperature needed, the bacteria would be able to be observed fairly quickly as they would grow exponentially in this nutrient rich environment.
Can a strict aerobe be distinguished from an anaerobe in thioglycollate broth?
Yes. Thioglycollate is a reducing medium that can establish a gradiation in oxygen content. Oxygen is highest at the top of the tube and absent in the deeper regions.Source: "Foundations in Microbiology" by Kathleen Park Talaro.
Why will obligate anaerobes grow in thioglycollate?
Obligate anaerobes do not need or use oxygen as a nutrient. In fact it is a toxic substance to them which either kills or inhibits their growth. Thioglycollate allows obligate anaerobes to grow because it consists of sodium thioglycollate, thioglycollic acid and L-crystine which reduce oxygen to water. This creates an anaerobic environment allowing obligate anaerobes to grow.
Does b.subtilis facultative anaerobes in thioglycollate broth?
No
Why are cysteine and sodium sulfide added to the growth media?
To culture obligate anaerobes, all molecular oxygen must be removed and kept out of the medium. Addition of oxygen-binding agents such as thioglycollate, the amino acid cysteine, or sodium sulfide to the medium prevents oxygen from killing the anaerobes you want to culture.
What type of media is used to demonstrate oxygen requirements of microbes?
thioglycollate
What does Thioglycollate contribute to culture media?
Look for this information in the link below.
