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Why you use Fluid Thioglycollate medium for bacterial contamination checking?
Wiki User
∙ 13y ago
Fluid Thioglycollate is a medium used to grow and observe all manner of aerotolerance in bacteria. Therefore if you were to place a sample in it to see if it contained bacteria or not, given the correct temperature needed, the bacteria would be able to be observed fairly quickly as they would grow exponentially in this nutrient rich environment.
Wiki User
∙ 13y ago
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The properties and purposes of thioglycollate medium?
A thioglycollate medium is used for culturing anaerobic bacteria.
How can you tell if thioglycollate medium is prepared correctly?
Thioglycollate medium is correctly prepared when a red/brown color appears at the top of the broth. This is beacuse of the Reazurin which is reactive to oxygen.
What is the function of thiogllycollic acid in thioglycollate?
It removes trapped oxygen from the medium
How does thioglycollate work?
Thioglycollate broth is an enriched medium used to determine the oxygen requirements of microorganisms. The diffusion of oxygen from the top of the broth provides a range of concentrations.
Can a strict aerobe be distinguished from an anaerobe in thioglycollate broth?
Yes. Thioglycollate is a reducing medium that can establish a gradiation in oxygen content. Oxygen is highest at the top of the tube and absent in the deeper regions.Source: "Foundations in Microbiology" by Kathleen Park Talaro.
Why use fresh thioglycollate medium?
A fluid thioglycollate is preferably used soon after preparation to minimize the amount of oxygen that is permitted to diffuse towards the bottom of the medium. The longer you wait before using this medium, the more oxygen is allowed to diffuse into it; more oxygen will negatively affect the growth of obligate anaerobes but would positively affect the growth of aerobic organisms.
Which of these algae are used to prepare a medium for bacterial culture?
Red Algae ?
What limits bacterial growth within a culture of medium?
Nutrients and oxygen
What is the purpose of flaming the loop before and after use?
Inoculating loop is used to inoculate microbial colony or sample on culture medium and to avoid the undesired microbial cells or to avoid contamination flaming of inoculating loop is necessary it is also called as incerination.
What is bacterial suspension?
Refers to the bacteria suspended on the petri dish or growth medium.
What is it called when feces are placed in a growth medium for bacterial analysis?
stool culture
Why are cysteine and sodium sulfide added to the growth media?
To culture obligate anaerobes, all molecular oxygen must be removed and kept out of the medium. Addition of oxygen-binding agents such as thioglycollate, the amino acid cysteine, or sodium sulfide to the medium prevents oxygen from killing the anaerobes you want to culture.
