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Why would E coli and B subtilis remain pink during gram staining?
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During the performance of the simple staining procedure you failed to heat fix your EColi smear preparation Upon microscopic examination how would you expect this slide to differ from correct slide?
The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
Why do you look at cultures that had been grown for different lengths of time during endospore staining?
You would look at cultures that had been grown for different lengths of time during endospore staining to ensure that the cells had been agitated enough to soak up the dye. This is done at 12, 24, and 36 hours.
Describe the microscopic appearance of encapsulated streptococcus if stained with safranin?
Safranin (red) is used in gram staining and endospore staining as the secondary stain. Nigrosin is used in negative staining, staining only the background and not the bacteria. Therefore, the bacteria within the capsule would stain red from the safranin. (Like in endospore staining and negative gram staining, safranin would stain the bacteria red.) Nigrosin would stain the background of the organism just as it would in negative staining. Bacteria (within capsul): stained safranin red Capsule (outer layer of bacteria): clear Background of organism: stained dark with Nigrosin
When staining ecoli with methylene blue what would you see?
blue bacilli
What happen in a cell if production of cyclin is halted during a cell cycle?
The cell would remain in the G2 phase and would not enter into mitosis
What is made by staining and arrganging chromosomes so that they can be viewed microscopically?
A karyotype is made by staining and arranging chromosomes so that they can be viewed microscopically.
What would be the effect of removing the mordant from the gram's staining procedure?
there is no real effect
If you were called away during the electrophoresis procedure and were not able to monitor your sample run what would happen if the electricity were to remain running in your absence?
you would die
Brown staining on teeth following wisdom teeth extraction?
More than likely, that is just dried blood. Nothing else would cause staining in removal of wisdom teeth.
What leukocyte is formed in bone marrow and having neutral staining granules?
That would be a Neutrophil. :)
When tea staining paper do you tea stain or write first?
write on it first, just make sure when your tea staining it the teabag you're using isn't soaking wet, as this would smudge the ink
How would you define a properly prepared bacterial smear?
the bacteria are evenly spread out on the prepared slide in such a concentration that they are adequately separated from one another bacteria are not washed off the slide during staining bacterial form is not distorted
