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Why you are using enzyme blank and substrate blank instead of using a single blank to find out the enzyme activity?
Wiki User
∙ 9y ago
I am working on pectinase enzyme assay. I incubated 900 ul of substrate for 10 minutes in the water bath, followed by adding 2ml of DNSA reagent, then 100ul of enzyme extract added finally i read the absorbance @ 540 OD. However the values are high. How can I troubleshoot high enzyme blank values in Pectinase assay ?
Wiki User
∙ 9y ago
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How many substrate molecules does a typical enzyme act on per second?
A single enzyme molecule can act on about 1000 substrate molecules per second.
How is a specific enzyme able to catalyze a specific reaction in the cytoplasm of a single cell with many different enzymes?
An enzyme binds to a specific substrate (reactant) for the reaction catalyzed.
How many substrates can an enzyme have?
A given enzyme works on ONE TYPE of substrate
How enzyme lowering the activation energy?
Enzymes lower activation energy by providing the substrates with an ideal environment for the certain reaction. Often, certain parts of the enzyme chain in the activation site will make the substrate more unstable by binding to it. This instability makes it easier to break down the substrate, or attach another molecule to it.
Does the smooth endoplasmic reticulum have a single or double membrane?
yeh it is a double membrane bound organelle. the inner membrane is heavily folded for a large surface are for enzyme activity. its function is the synthesis, modification and distribution of protiens.
How many substrate molecules does a typical enzyme act on per second?
A single enzyme molecule can act on about 1000 substrate molecules per second.
How is a specific enzyme able to catalyze a specific reaction in the cytoplasm of a single cell with many different enzymes?
An enzyme binds to a specific substrate (reactant) for the reaction catalyzed.
How many substrates can an enzyme have?
A given enzyme works on ONE TYPE of substrate
How fast do enzymes act?
Enzymes reaction cycle is so fast that a single enzyme molecule typically act on about thousand substrate molecules per second.
Do enzymes work only on single substrate in most cases?
Yes, almost all work on a single substrate.
How enzyme lowering the activation energy?
Enzymes lower activation energy by providing the substrates with an ideal environment for the certain reaction. Often, certain parts of the enzyme chain in the activation site will make the substrate more unstable by binding to it. This instability makes it easier to break down the substrate, or attach another molecule to it.
Do enymes brake down lipids carbohydrates and prptiens?
Yes specific enzymes are breaking down carbohydrates, proteins and lipids to form sugars, amino acids and fatty acids respectively by hydrolysis. All enzymes are specific for a particular substrate. Not a single enzyme can do all these processes!
What is the scientific name of enzymes?
Enzymes belong to a class of proteins called "biocatalysts" and do not have a single scientific name. They are named based on the substrate they act upon and end with the suffix "-ase." For example, the enzyme that breaks down proteins is called protease.
Does the smooth endoplasmic reticulum have a single or double membrane?
yeh it is a double membrane bound organelle. the inner membrane is heavily folded for a large surface are for enzyme activity. its function is the synthesis, modification and distribution of protiens.
How many times can we use a human enzyme in the lab for industrial purposes?
I think this question can not be answered completely. Even in a single step an enzyme reacts a thousand or million times with his substrate. In a following step of recycling you`ll lose an unknown amount of your enzyme, depending on the methods. After that some enzymes should be still there but the velocity or efficiency in the next steps is decreasing constantly. If you accept slower production you can us it nearly infinitive but usually you add sometimes new enzymes....
What are the structures called that anchor bread molds to molds to their food substrate?
rhizoids
List two diseases caused by the absence of a single enzyme in an organism?
protein is an enzyme due to lack of protein SCID Thalassemia
