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Q: Why you use ice-cold condition in the cell lysis?
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Do apocrine glands use exocytosis to make secretions?

No holocrine glands release their secretions by cell lysis, the entire cell disintegrates.


Function of Triton X-100 in Lysis buffer?

It dissolves the cell mebrane. Keep in mind that some people do not use Triton X-100 in cell lysis, but instead sonificate... Also Triton X-100 can be replaced by a whole lot of other stuff (CHAPS, Igepal, etc) Hope to have givin you enough info


What is the use of alkaline lysis solution 1 in plasmid isolation?

it helps to homogenize the cell and give single cell suspension


Why is gram negative bacteria less susceptible to osmotic lysis?

Because penicillin disrupts and destroys the peptidoglycan layer. Gram Negative bacteria have an outer membrane that blocks penicillin from getting to is peptidoglycan. Gram positive bacteria do not have this outer membrane, its peptidoglycan layer is out and exposed!!


What is the use of resuspension solution in DNA isolation?

Resuspension buffer (solution I) is used for the isolation of plasmid DNA by alkaline lysis method. Bacterial cells, obtained from the culture (liquid culture or colonies grown on agar plate), is resuspended in this buffer. The purpose of this buffer is to provide an optimal starting pH (pH 8.0) and an ideal condition for subsequent lysis.


What is Buffer ATL?

Buffer ATL is a proprietary chemical used for DNA extraction and purification. It's used to induce lysis of cell tissues in order to release and expose the cell's DNA during the beginning stages of of the extraction process. I think the letters "ATL" stand for "Animal Tissue Lysis." It's part of the DNeasy protocol from Qiagen. I use this every day during my extraction and purification of DNA from various snake species.


What is the function of TNE buffer?

We use it for isolation of proteins from yeast cells as a lysis buffer


What cpt code do you use for a laparotomy lysis of adhesions with left salpingo oophorectomy?

58720


Under what condition does a cell Use fermentation instead of cellular respiration?

low oxygen


What is cryptic growth?

Death Regeneration, including growth and lysis. Lysis followed by growth on the secondary substrate arising from the decay. Some microorganism (mo) may not able to use lysate for cryptic growth.


What word is use for a disease condition of any blood cell?

blood dyscrasia


What is the difference between cell potential and standard cell potential?

the standard cell potential is the cell potential at standard conditions (25C , 1 atm , and 1M ) but the cell potential is the cell potential of the cell under a real condition and we use nernst equation . i hope this is useful