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All samples must be under 24 hours old or you might get an incorrect reading.
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Why is it necessary to use a young culture 24-hr old culture in gram staining?
less than 24 hours old. Older cultures tend to lose the ability to retain stains.
What gram reaction would you expect if you used a culture that was growing for 72 hours?
Gram negative
What is a gram variable bacteria?
gram-positive bacteria but can become gram negative if the smear is left to culture for a long period of time.
Why is time an important factor in simple staining?
In Gram staining, some bacterial species will become Gram variable meaning they produce a false reaction for the following reasons: decolrized too long, too young/old of culture, stained too long. Also, time is important simply because you don't want to over or under stain. You won't be able to see your specimen as well.
Why would a gram stain be ordered during surgery?
to determine a type of bacteria. This question is very vague. I will assume they are doing surgery on something that is infected and to help identify the the bacteria causing the infection, gram stain would be ordered.
Why must young cultures be used when doing staining?
Young cultures must be used when doing a gram stain to get more accurate results. The cell was is the part of the bacterial cell that is most involved with gram staining because it holds the crystal violet.
Why is it necessary to use a young culture 24-hr old culture in gram staining?
less than 24 hours old. Older cultures tend to lose the ability to retain stains.
Why must fresh bacterial cultures be used in a Gram stain?
Why must young cultures be used when doing a Gram stain Young cultures must be used so the crystal violet can stick to the cell walls of Gram positive bacteria. The cell walls break down in old cultures and the staining process is not accurate
Would it be useful to perform a gram stain on a mixed culture?
The purity of a culture of bacteria is important so it can test on that one type of bacteria. Gram staining can be good so you make sure everything in the streak plate is one color showing that it is gram positive and gram negative.
What gram reaction would you expect if you used a culture that was growing for 72 hours?
Gram negative
What is a gram variable bacteria?
gram-positive bacteria but can become gram negative if the smear is left to culture for a long period of time.
What is S Acin in a urine culture report?
what does a report mean when is say 1,000-10,000 CFU/ml of Gram positive cocci and Gram negative bacilli. Is this a positive culture?
Suppose you are viewing a Gram stained field of red rods and purple cocci through the microscope What do you conclude?
The rods will be gram negative, the cocci are gram positive: most likely, the slide was made from a mixed culture of bacteria, or the culture was contaminated.
Sputum Culture-gram positive cocci?
Sounds pretty typical.
Why are contrasting colors important in gram staining?
# Suppose you performed a gram stain on a sample from a pure culture of bacteria and observed a friend of red and purple cocci. Adjacent were not always the same color. What do you conclude? # ## The bacteria was not a pure culture.
Is B subtilis a gram positive or gram negative bacterium?
gram positive Exactly. When doing a gram stain on B. subtilis, this bacterium resists decolorization (keping the first stain and NOT taking on the color of the secondary stain). Therefore, this bacterium is gram (+).
How is the Gram stain technique be of value in establishing purity of a culture of bacteria?
deffirent bacteria show different response to gram stainig procedure so bacteria are classified into two groups:a(gram positive bacteria)b(gram negitive bacteria)
