by autoclaving at 121°C for 15 minutes
use a pressure cooker
yes
it is unacceptable
BSA solution is Bovine Serum Albumin
BSA Company was created in 1978.
Autoclaving is used to kill all micro-organisms that are in the culture. Autoclaving is usually carried out at 121 deg C with steam under 2 atmospheres of pressure. A clean culture free of all micro-organisms is essential as you only want the organism of intrest to grow.
http://wiki.answers.com/Q/How_are_the_material_prepared_for_autoclaving"
Autoclaving
Autoclaving is more efficient than boiling because it has a hotter temperature. This is sure to clean the tools correctly by sterilizing and remove all germs.
One can purchase BSA optics from various websites like Amazon or eBay. One can also buy BSA optics directly from the official BSA optics website itself.
autoclaving