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Would a change starting at a pH of 7.8 alter the EMB medium's sensitivity or specificity?
Wiki User
∙ 11y ago
Eosin methylene blue agar (EMB) is a selective and differential medium used to isolate fecal coliforms. Eosin Y and methylene blue are pH indicator dyes which combine to form a dark purple precipitate at low pH; they also serve to inhibit the growth of most Gram positive organisms. Sucrose and lactose serve as fermentable carbohydrate sources which encourage the growth of fecal coliforms and provide a means of differentiating them. If the pH is changed (even at the beginning) the test might be ''read" incorrectly and the improper medication will be given. Always follow instructions for this reason.
Wiki User
∙ 7y ago
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The alteration of an amino acid on a site other than the active site will: change the shape of the protein.
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