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Would removing eosin Y from methylene blue from EMB agar alter the medium sensitivity or specificity?
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Quantity of lactose in levine eosin methylene blue agar?
Levine eosin methylene blue agar does not contain lactose. It is a selective and differential medium used for the isolation and differentiation of gram-negative enteric bacteria based on their ability to ferment lactose. The agar contains lactose as a substrate for fermentation, but the actual quantity of lactose present in the agar may vary depending on the manufacturer and the specific formulation of the medium.
EMB is a selective medium Is it also a defined or an undefined medium Why is that formulation desirable?
EMB is an undefined selective/differential medium. It contains aniline dyes (methylene blue and eosin), which inhibit the growth of Gram-positive bacteria selecting for Gram-negative bacteria. EMB also contains lactose which makes the media differential based on an organisms ability to ferment lactose
Definition of culture and sensitivity tests?
A collection of a urine sample, blood sample or other body fluid samples are cultured in medium and analysed with for sensitivity. A "C&S" is a diagnostic lab procedure used to identify the type of bacteria and to determine which antibiotics can successfully fight an infection. C&S is primarily used to find bacteria. The absence of bacteria does not mean there is no infection, since it could be a virus that will not grow in a specific culture medium.
Can a single medium function for both differentiation and selectivity?
yes...by removing certain growth factors such as amino acid,vitamins etc which would inhibit the growth of a desired organism
What is a rarer medium?
If the light travels faster in the second medium, then this medium is called the rarer medium.
How would removing the crystal violet from MacConkey agar alter the sensitivity and specificity of the medium?
It would allow growth of Gram-positive cocci.
Would omitting NaCl alter the medium's specificity or sensitivity?
The answer is Yes, only if this is in reference to a selective and/or differential medium. Specificity is the ability of the test to identify negative results while sensitivity is the ability of a test to detect positive results. So, if taking out the NaCl effects your ability to interpret your data results then it in someway has effected your sensitivity and specificity.
Would a change starting at a pH of 7.8 alter the EMB medium's sensitivity or specificity?
Eosin methylene blue agar (EMB) is a selective and differential medium used to isolate fecal coliforms. Eosin Y and methylene blue are pH indicator dyes which combine to form a dark purple precipitate at low pH; they also serve to inhibit the growth of most Gram positive organisms. Sucrose and lactose serve as fermentable carbohydrate sources which encourage the growth of fecal coliforms and provide a means of differentiating them. If the pH is changed (even at the beginning) the test might be ''read" incorrectly and the improper medication will be given. Always follow instructions for this reason.
How does adding the sterile mineral oil affect the specificity or the sensitivity of the Decarboxylation Test?
When you add mineral oil for Decarboxylation test you do not need a thick layer of oil, you only need enough to cover the surface of the medium. This promotes fermentation by locking out oxygen, and it also prevents false alkalinization at the surface of the medium. Hopefully my answer can help you. =)
Does poor sensitivity or poor specificity cause a false positive from the reaction between inoculating loop and hydrogen peroxide?
Catalase is the enzyme that catalyzes the breakdown of hydrogen peroxide, which in result, turns into water and oxygen. However, if catalase is presence in the medium, it may lead to false positive results.
What is the default sensitivity on modern warfare 2?
It is set as medium, which is at 2.
Quantity of lactose in levine eosin methylene blue agar?
Levine eosin methylene blue agar does not contain lactose. It is a selective and differential medium used for the isolation and differentiation of gram-negative enteric bacteria based on their ability to ferment lactose. The agar contains lactose as a substrate for fermentation, but the actual quantity of lactose present in the agar may vary depending on the manufacturer and the specific formulation of the medium.
What make poetry an effective medium of expression?
Specificity strengthens a poem One should focus on accurately communicating an aspect of your experience, instead of focusing on what impression you are making when you do it.
How do you use specificity in a sentences?
Somewhere in a sentence. Specifically, at the beginning. :D
EMB is a selective medium Is it also a defined or an undefined medium Why is that formulation desirable?
EMB is an undefined selective/differential medium. It contains aniline dyes (methylene blue and eosin), which inhibit the growth of Gram-positive bacteria selecting for Gram-negative bacteria. EMB also contains lactose which makes the media differential based on an organisms ability to ferment lactose
Is emb a defined medium?
EMB is an undefined selective/differential medium. It contains aniline dyes (methylene blue and eosin), which inhibit the growth of Gram-positive bacteria selecting for Gram-negative bacteria. EMB also contains lactose which makes the media differential based on an organisms ability to ferment lactose.
Does bacillus grow on EMB agar?
no way to grow the specious ,because the medium containing dye and ions ,indicator .for the growth it required salt and carbon ,nitrogen and micro nutrients.
