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You maked phosphate buffer white pH 8.5 and then store in refrigerator and become crystallin why?
Rasoul
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Phosphate buffer temperature is maintained at what?
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If use sodium phosphate buffer instead of potassium phosphate buffer?
its ok to use
What is the difference between phosphate buffer and phosphate buffered saline?
actualy there is no major difference , on the basis of composition ,can be differentiate in saline buffer nacl was used and isotonicity take place
Why is the phosphate buffer made up by using the Henderson hasselbalch equation not the expected PH?
1. Why is the phosphate buffer made up by using the Henderson-Hasselbalch equation not the expected pH?
What is the role of disodium phosphate dihydrate in insulin?
Disodium hydrogen phosphate dihydrate has the role of a buffer in some insulin receipts.
How do you prepare phosphate buffer of pH 7.5?
See the Related Links for "Columbia.edu: Phosphate buffer automatic calculator" to the bottom for the answer.
If use sodium phosphate buffer instead of potassium phosphate buffer?
its ok to use
How to prepare phosphate buffer pH 5.8?
To prepare a phosphate buffer solution at pH 5.8, mix the appropriate amounts of monosodium phosphate (NaH2PO4) and disodium phosphate (Na2HPO4) in water. The exact ratio will depend on the desired buffer capacity. Adjust the pH by adding small amounts of acid or base as needed, and then confirm the pH using a pH meter.
How you prepare 10 mM phosphate buffer from 0.5 M phosphate buffer?
By dilution of 1 part 0.50M buffer with 49 parts of water, giving 50 parts of the desired 0.010M = 10 mM Phosphate buffer
How I can prepare 5 mM phosphate buffer?
To prepare a 5 mM phosphate buffer, first calculate the amount of monosodium phosphate (NaH2PO4) and disodium phosphate (Na2HPO4) needed based on their respective molecular weights. For example, to make 100 ml of solution, dissolve 0.190 g of NaH2PO4 and 0.281 g of Na2HPO4 in distilled water. Adjust the pH to your desired range, typically around 7.4, by adding small amounts of acid or base.
Why is isolated DNA suspended in TE buffer only instead of any buffer like phosphate buffer or so?
Tris EDTA buffer inhibits nucleases that will degrade the DNA, by chelating cations required by the enzymes. Phosphate buffer offers no such protection against degradation.
Which buffer system acts the fastest?
bicarbonate buffer is instant, followed by respiratory, renal, and phosphate.
What is the difference between phosphate buffer and phosphate buffered saline?
actualy there is no major difference , on the basis of composition ,can be differentiate in saline buffer nacl was used and isotonicity take place
What are different types of buffering system?
1. Bicarbonate buffer system 2. Protein buffer system 3. Phosphate buffer system
How do you make 20 mM potassium dihydrogen phosphate buffer with only dihydrogen?
Without phosphate you can't.
What happens when you add NaCl salt in it's solid state to a phosphate buffer system?
When you add NaCl salt in its solid state to a phosphate buffer system, it will dissolve in the buffer solution and dissociate into Na+ and Cl- ions. The presence of NaCl may slightly affect the ionic strength of the solution, but it should not significantly alter the buffering capacity or pH of the phosphate buffer system.
Why is the phosphate buffer made up by using the Henderson hasselbalch equation not the expected PH?
1. Why is the phosphate buffer made up by using the Henderson-Hasselbalch equation not the expected pH?
What is the principle buffer in the body?
The principle buffer in the body is the bicarbonate buffer system, which helps regulate the pH of the blood. This system works by converting carbonic acid to bicarbonate ion and vice versa, depending on whether the blood pH needs to be decreased or increased.
