answersLogoWhite

0

What else can I help you with?

Related Questions

Why it is necessary to spray the chromatography with ninhydrin?

Spraying chromatography with ninhydrin helps visualize and detect amino acids or peptides on the chromatogram through a purple color reaction. It is necessary to enhance sensitivity and allow for better quantification of the compounds separated on the chromatography plate.


Why you will spray ninhydrin for analysis in chromatography?

Since amino acids are colourless compounds, ninhydrin is used for detecting them. To identify this, after development, the TLC plate is sprayed with ninhydrin reagent and dried in an oven, at 105°C for about 5 minutes. Ninhydrin reacts with α- amino acids that results in purple coloured spots [(due to the formation of the complex - Rheuman's purple).


What is the molecular formula of ninhydrin?

Well, the best I could come up with is it's either:C9H6O2orC4H3O2If someone knows any different please correct me.jman63: it is actually C9H6O4


Does ninhydrin has flaws?

Yes, ninhydrin has some drawbacks. It can react with other substances present in the sample, leading to false positive results. Additionally, the reaction with ninhydrin is not specific to a particular amino acid, which can limit its application in identifying specific amino acids.


Is ninhydrin the same as super glue?

NO, ninhydrin is not the same as super glue


What is the boiling point of ninhydrin?

The boiling point of ninhydrin is approximately 275-280°C.


What does a cromotogram tell you about the colours in the clorophyll?

A chromotogram shows the different pigments present in chlorophyll, with each pigment represented by a distinct band. This helps identify the specific colors present in the chlorophyll sample based on the separation of pigments. Different pigments will show up as different colored bands on the chromotogram.


How is ninhydrine reagent prepared?

Ninhydrin reagent is prepared by dissolving ninhydrin powder in a solvent such as ethanol or acetone. The solution is typically heated gently to aid dissolution. It is important to prepare fresh ninhydrin reagent before use to ensure its effectiveness in detecting amino acids.


Would ninhydrin test be reliable in determining the presence of proteins?

No! because the Ninhydrin test is mostly used to detect ammonia or primary secondary amines


What items are necessary for bug catching?

net and insect spray


What does ninhydrin do to the fingerprints?

Ninhydrin reacts with amino acids present in the sweat from fingerprints, producing a purple or blue color. This allows for the visualization and development of latent fingerprints on surfaces.


What is the principe of ninhydrin test?

The principle of the ninhydrin test is based on the reaction of ninhydrin with amino acids or proteins to form a purple or blue color complex. This test is commonly used to detect the presence of amino acids in a sample, making it a useful tool in protein analysis and forensic science.