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Q: Why it is necessary to spray the chromotogram with ninhydrin?
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Why it is necessary to spray the chromatography with ninhydrin?

because it is a locating agent that identify the different substances , espically the invisible amine acid


Why you will spray ninhydrin for analysis in chromatography?

Since amino acids are colourless compounds, ninhydrin is used for detecting them. To identify this, after development, the TLC plate is sprayed with ninhydrin reagent and dried in an oven, at 105°C for about 5 minutes. Ninhydrin reacts with α- amino acids that results in purple coloured spots [(due to the formation of the complex - Rheuman's purple).


Is ninhydrin the same as super glue?

NO, ninhydrin is not the same as super glue


Does ninhydrin has flaws?

Yes ninhydrin does have flaws. The most common flaw of ninhydrin is the fact that it is so sensitive to the extent of not recording a fingerprint if enough perspiration wasn't created.


What is the molecular formula of ninhydrin?

Well, the best I could come up with is it's either:C9H6O2orC4H3O2If someone knows any different please correct me.jman63: it is actually C9H6O4


Result of casein in ninhydrin test?

Kharkov's


Would ninhydrin test be reliable in determining the presence of proteins?

No! because the Ninhydrin test is mostly used to detect ammonia or primary secondary amines


What items are necessary for bug catching?

net and insect spray


Is Ninhydrin used to test for the presence of lipids?

No, a grease spot test or Sudan IV test can be used to test for lipids. Ninhydrin is used to test for amino acids.


Which chemical is used by police to find bribe?

One chemical that can be used by police to detect traces of bribe money is called 2,4-Dinitrophenylhydrazine (2,4-DNPH). This chemical reacts with the substances often found in banknotes and produces a color change, making it easier to identify tainted money.


What is ruhemanns purple formed in ninhydrin reaction?

It is the final coloured compound.


Why is it necessary to spray the chromatogram with resorcinol?

Resorcinol is a colouring agent for some chromatography experiments.