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move the stage up and down slowley.
An image can appear blurred under a microscope due to improper focusing, contamination on the microscope lens or slide, incorrect magnification setting, or presence of air bubbles in the viewing field. Proper cleaning and adjustment of the microscope components can help improve image clarity.
first you turn magnification lens to lowest setting. second, you remove the slide you were looking at. third, you turn off or unplug the microscope.
Moving the 10x objective into place when putting the microscope back into the cabinet helps protect the more delicate higher-power objectives from potential damage. The 10x objective has a longer working distance, reducing the risk of contact with the slide or stage during storage. Additionally, it ensures that the microscope is in a low-power setting, which is safer for both the equipment and any remaining specimens. This practice also makes it easier to set up the microscope for future use.
Short answer: Zacharias Jansen Long answer: Your question is not valid for 2 reasons: 1. You cannot "discover" something if it does not exist. You should be asking who invented it, not who found it laying around somewhere. 2. A "simple microscope" is not common terminology. Simple compared to an electron microscope? or simple compared to a compound microscope? What is typically referred to as just "microscope" is technically a compound microscope. A set of multiple lenses mounted in a desktop style that allows the compounding of magnification. A compound microscope is the standard microscope in any basic lab setting. Anything more "simple" than a compound microscope would not even really be a microscope, it would either be a telescope, or simpler than that is a magnifying glass (with a single lens) The inventor of the magnifying glass was: Roger Bacon The next step up is the telescope invented by: Zacharias Jansen The next step up is the "compound microscope" which was also invented by: Zacharias Jansen (this is the simplest form of what would be recognized as or named "microscope") If you wanted to go even "simpler" and define microscope as anything that magnifies, there were reading glasses around for thousands of years prior, and even "reading stones" which were lumps of polished glass used to magnify parchment in Egypt as far back as 7000 B.C. (inventor unknown). So it really depends on how you wish to refine your meaning of simple. The magnification of anything? There is no known inventor for reading stones, his name is lost to time. Or if you mean the first invented microscope that could examine things too small for a human eye to detect, that's a compound microscope. Thus if I am guessing your meaning correctly, you meant to ask this question: Question: Who invented the compound microscope? Answer: Zacharias Jansen
The lightest setting for a specimen in microscopy is typically achieved using the lowest power objective lens, as it allows for greater working distance and reduced light intensity. Additionally, adjusting the diaphragm to a wider opening can enhance light transmission, making the specimen appear lighter. Properly adjusting the condenser can also contribute to optimizing light exposure, resulting in a clearer and lighter view of the specimen.
Having the light intensity setting too high on a light microscope can lead to overheating the specimen, causing potential damage. It can also result in bleaching of fluorescent dyes used in the specimen, affecting the quality of images produced. Maintaining the appropriate light intensity setting ensures accurate observations and prevents unnecessary harm to the specimen.
move the stage up and down slowley.
When first starting to use a microscope, it is best to use the diaphragm setting at its widest opening. This allows the maximum amount of light to pass through the specimen, making it easier to see details clearly. Once you have focused on the specimen, you can then adjust the diaphragm to optimize contrast and clarity based on your observation needs.
An image can appear blurred under a microscope due to improper focusing, contamination on the microscope lens or slide, incorrect magnification setting, or presence of air bubbles in the viewing field. Proper cleaning and adjustment of the microscope components can help improve image clarity.
When setting up a microscope, you typically start with the lowest power objective lens first, such as the 4x objective lens. This lens allows you to find and focus on the specimen at a lower magnification before switching to higher power objectives for more detailed viewing.
For a scanning electron microscope, the most commonly used type of EM, there is no need to do anything other than stick it to an electrically conducting pad. If the sample is particularly insulating, it may be worth coating it with a very thin gold layer to prevent the specimen from charging up with electrons from the beam (this would blur the image) For a transmission electron microscope, the specimen is most commonly encased in epoxy resin before being sliced into thin enough slices that the electron beam can pass through. The sample is then placed on a fine copper grid before being placed in the microscope.
You should first use the lowest power setting of a microscope to find an object and then center it within the viewing area. Then, when you switch to the next higher magnification, your target should still be close to the center and easier to find and focus on.
The vernier adjustment is numbered. Go to the lowest point in the object you can focus on. Note your vernier setting. Adjust to the highest point you can focus on. Subtract the lower number from the higher and you get depth.
The fine adjustment, the two little knobs on both sides of a compound microscope, is rotated to move the body tube down or the stage up in tiny distances to help you focus in HIGH power.The fine focus knob is geared with a different ratio than the coarse knob to "fine tune" your focus. It allows for tiny adjustments to the focus setting, rather than larger ones.
There are two types of diaphragms of a microscope. There are disk and iris type diaphragms, the setting are dependent upon the transparency of the object, the degree of contrast desired and the objective lens selected.
To regulate the diaphragm of a microscope, you can typically adjust a small lever or wheel located beneath the stage of the microscope. This allows you to control the amount of light passing through the specimen and into the objective lens. By opening or closing the diaphragm, you can adjust the contrast and brightness of the image being viewed, helping to improve clarity and visibility. It is important to optimize the diaphragm settings based on the specific characteristics of the specimen being observed.