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A misfire in a 2001 Subaru 2.5-liter four-cylinder engine can be caused by several factors, including faulty spark plugs, worn ignition coils, or issues with the fuel system, such as clogged fuel injectors or a failing fuel pump. Additionally, vacuum leaks, compression problems, or malfunctioning sensors like the mass air flow or oxygen sensor can also contribute to misfires. Regular maintenance and diagnostic checks can help identify and resolve these issues.

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