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To check the separation of dye, I employed a technique called chromatography. This involved applying a small amount of the dye onto a stationary phase, such as filter paper, and then allowing a solvent to travel up the paper, carrying the dye with it. By observing the distance each color moved, I was able to determine the separation and composition of the dye mixture. This method effectively showcased how different components of the dye interacted with the solvent and stationary phase, revealing their distinct properties.

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How were you able to check the separation of dye in chromatography?

To check the separation of dye in chromatography, I observed the movement of the dye spots along the stationary phase as the solvent traveled up the medium. By measuring the distance each dye traveled relative to the solvent front, I calculated the Rf values, which helped determine how effectively each dye separated. Additionally, visual analysis of the distinct spots or bands indicated the degree of separation achieved. This process allowed for a clear assessment of the chromatographic efficiency and the characteristics of the dyes used.


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