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Why the retention time will decrease if the polarity of mobile phase increase in normal phase HPLC?
because in normal phase HPLC mobile phase is non polar and stationary phase is polar. Most of the compound of interest are polar, if you increase the polarity of mobile phase compound of analyte will stay in mobile phase and will elute faster and retention time will be shorter.
How do you prepare analyte?
The substance to be analysed normally of unknown quantity is called as analyte. it is called as sample. In titration of analytes normally we take it according to the Normality of the titrant taken to find the quantity of analyte. Sample Size = Titer Value*Normality*Molecularr(or)equivalent weight/ purity/10. This formula is expressed in terms of %.
In a titration what two things are always equal to each other at the endpoint?
In a titration, the moles of the titrant added are equal to the moles of the analyte in the solution at the endpoint. This equality is essential for determining the concentration of the analyte in the solution.
What is a standard addition calibration?
Standard addition calibration is another method of calibration (used to determine concentrations of sample substances). It is used or perhaps preferred by most chemist because due to some interference the analytical response of an analyte in a complex sample may not be the same as for the analyte in a simple standard. This means that this method will lessen the inaccuracies in the experiment and therefore a more accurate calculation of the concentration of a substance. Standard addition is performed in a variety of ways that consist of adding known amounts of analyte into the complex mixture and associating it with the response. Performing enough associations allows one to mathematically predict the effect the complex matrix exerts and subsequently calculate the amount of analyte in a native sample.
Difference between iodometry and iodimetry?
When an analyte that is a reducing agent is titrated directly with a standard iodine solution, the method is called "iodimetry". When an analyte that is an oxidizing agent is added to excess iodide to produce iodine, and the iodine produced is determined by titration with sodium thiosulfate, the method is called "iodometry".
Why the retention time will decrease if the polarity of mobile phase increase in normal phase HPLC?
because in normal phase HPLC mobile phase is non polar and stationary phase is polar. Most of the compound of interest are polar, if you increase the polarity of mobile phase compound of analyte will stay in mobile phase and will elute faster and retention time will be shorter.
How do you calculate hplc assay and hplc purity?
HPLC purity :It explains how pure our analyte is in the given mixture .It is not related to the how much our analyte is in the given mixture.i.e Percentage of a our analyte with out impuritys in HPLC.(Known or Unknown)HPLC assay :It explains how much is our analyte in the given mixture(The content of our component in the given mixture).It is not related to analyte purity.HPLC potency :It is measurement of our analyte how potent it is.i.e Purity of our analyte with out all possible impuritys like chromatographic impuritys(HPLC,GC-Residual solvents,TLC),heavy metals,sulphated ash ..etcFor example:If we have a analyte of some X of purity 99.5%.Prepare 20%,60% and 90% of solution of X.inject all these solution in hplc.For 20% solution you will get 99.5% purity and 20% assay.For 60% solution you will get 99.5% purity and 60% assayFor 90% solution you will get 99.5% purity and 90% assay.
How buffer concentration effects on retention time in hplc method?
Buffer concentration can affect retention time in HPLC by influencing the pH of the mobile phase, which can in turn impact interactions between the analyte and stationary phase. Higher buffer concentrations can alter the ionization state of the analyte, leading to changes in its retention time. Additionally, buffer concentrations can also affect peak shape and resolution in the chromatogram.
What is titrant and analyte?
Titrant is the solution of known concentration that is added to the analyte during a titration to determine its concentration. The analyte is the substance being analyzed in the solution that reacts with the titrant.
Is the indicator generally added to the titrant or analyte in a titration?
Analyte is the indicator that is generally added in titration.
Is the indicator generally added to the titrant or the analyte in titration?
Analyte is the indicator that is generally added in titration.
Is the indicator generally added to the titrant or the analyte in a titration?
Analyte is the indicator that is generally added in titration.
Define the analyte in a titration?
The analyte in a titration is the substance being measured or analyzed. It is the component of interest whose concentration is determined by reacting it with a titrant of known concentration until an equivalence point is reached.
How does back titration differ from a direct titration?
direct titration involves the direct and stepwise addition of a standard titrant to the analyte whilst the back titration involves reacting a standard excess titrant wth an analyte solution of an unknown concentration, then reacting the excess (left over) titrant with an analyte of known concentration to determine the concentration of excess titrant.
What is the titrant in the burette used for in a titration experiment?
The titrant in the burette is used to react with the analyte in the flask during a titration experiment to determine the concentration of the analyte.
What is the significance of the half equivalence point in a titration experiment?
The half equivalence point in a titration experiment is significant because it indicates the point at which half of the analyte has reacted with the titrant. This point helps determine the pKa of the analyte and can be used to calculate the concentration of the analyte in the solution.
How do you prepare analyte?
The substance to be analysed normally of unknown quantity is called as analyte. it is called as sample. In titration of analytes normally we take it according to the Normality of the titrant taken to find the quantity of analyte. Sample Size = Titer Value*Normality*Molecularr(or)equivalent weight/ purity/10. This formula is expressed in terms of %.
