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TENS buffer is made up of: Tris, EDTA, NaOH, and SDS.
Tris: buffer solution usually used for nucleic acids, makes sure that the pH stays fairly constant throughout procedure
EDTA: chelating agent, chelates positively charged ions like Mg2+ and Ca2+ that might harm the DNA
NaOH and SDS: disrupts cell membrane so you can get the DNA out
What else can I help you with?
What is tens buffer?
TENS stands for Transcutaneous Electrical Nerve Stimulation, a therapy that uses low-voltage electrical currents to alleviate pain. A TENS unit typically requires gel or conductive pads to transmit electrical signals to the skin, which helps stimulate nerve fibers and provide pain relief. This gel or conductive material acts as a buffer between the skin and the electrodes to improve the effectiveness of the treatment.
How is a human gene recombined into a bacterial plasmid?
One of the most common ways these days is from cDNA. RNA is extracted from human cells, purified, and then treated with an enzyme called reverse transcriptase which is able to make DNA from RNA templates (this DNA made from RNA is called cDNA). The advantage of using cDNA is that in the genome human genes are typically distributed across multiple exons spread over tens or even hundreds of thousands of basepairs of DNA. Such a massive segment of DNA is extremely hard to manipulate and far too large to insert into a plasmid. However, in cDNA, all the introns have been spliced out (because cDNA is made from mature mRNA). To isolate a particular gene from cDNA, PCR is often used to selectively amplify one gene's cDNA using specific primers. To insert the amplified cDNA into a plasmid, the traditional approach was to use restriction enzymes - enzymes that cut precise DNA sequences. The great thing about many restriction enzymes is that they cut DNA but leave behind "sticky ends". Thus if you cut both your cDNA and a plasmid with a particular restriction enzyme, the resulting sticky ends will allow the human cDNA to be taken up by the plasmid (the sticky ends will mesh). The sticky ends will have to be sealed by an enzyme called DNA ligase. However, there are other ways these days - often involving recombination to insert the PCR product directly into a plasmid without resorting to restriction enzymes and ligations.
How many proteins exist in the human body?
There are estimated to be tens of thousands of different proteins in the human body.
Can you treat vertigo with TENS machine?
While TENS machines are commonly used for pain relief, they are not typically recommended for treating vertigo. It's important to consult with a healthcare provider to determine the underlying cause of vertigo and the appropriate treatment plan, which may include specific exercises, medications, or other interventions tailored to your individual needs.
Does every gene code for one protein?
yes, a single gene can code for multiple proteins by the process of alternative splicing. DNA is transcribed into m-RNA and that is then translated into proteins. The pre m-RNA can be coded for at different sites, it can contain introns that create a different code, or it can have exons spliced from it.
What is tens buffer?
TENS stands for Transcutaneous Electrical Nerve Stimulation, a therapy that uses low-voltage electrical currents to alleviate pain. A TENS unit typically requires gel or conductive pads to transmit electrical signals to the skin, which helps stimulate nerve fibers and provide pain relief. This gel or conductive material acts as a buffer between the skin and the electrodes to improve the effectiveness of the treatment.
How much would a 30-06 bolt action cost?
A few hundred to tens of thousands.
What is a colt single action army serial 19923 0 worth?
A few hundred to tens of thousands of USD depending on EXACTLY what you have.
Why 17 tens and 1 hundred 7 tens have the same value?
17 tens = (10 + 7) tens = 10 tens + 7 tens = 1 hundred + 7 tens.
What is 42 tens divided by 6 tens?
7
What is 3 tens from 9 tens?
6 tens
What does 67 tens 10 tens equal?
To calculate 67 tens plus 10 tens, you first convert the tens into their numerical values. This gives you 670 (from 67 tens) plus 100 (from 10 tens), which equals 770. Therefore, 67 tens plus 10 tens equals 770.
What is 23 tens 8 tens?
31 tens(310)
What is 19 tens plus 13 tens?
190 + 130 = 320
How is a human gene recombined into a bacterial plasmid?
One of the most common ways these days is from cDNA. RNA is extracted from human cells, purified, and then treated with an enzyme called reverse transcriptase which is able to make DNA from RNA templates (this DNA made from RNA is called cDNA). The advantage of using cDNA is that in the genome human genes are typically distributed across multiple exons spread over tens or even hundreds of thousands of basepairs of DNA. Such a massive segment of DNA is extremely hard to manipulate and far too large to insert into a plasmid. However, in cDNA, all the introns have been spliced out (because cDNA is made from mature mRNA). To isolate a particular gene from cDNA, PCR is often used to selectively amplify one gene's cDNA using specific primers. To insert the amplified cDNA into a plasmid, the traditional approach was to use restriction enzymes - enzymes that cut precise DNA sequences. The great thing about many restriction enzymes is that they cut DNA but leave behind "sticky ends". Thus if you cut both your cDNA and a plasmid with a particular restriction enzyme, the resulting sticky ends will allow the human cDNA to be taken up by the plasmid (the sticky ends will mesh). The sticky ends will have to be sealed by an enzyme called DNA ligase. However, there are other ways these days - often involving recombination to insert the PCR product directly into a plasmid without resorting to restriction enzymes and ligations.
How do you write 3 tens and 0 tens?
3 tens is written as 30 0 tens is written a 0
How many tens are in the number 168?
16.8 tens, or 16 tens with 8 left over.
