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Some advantages are that it can create many copies of a minimal starting amount ie good for criminal investigations if you find some hair you can use PCR to find out whose it is. Also another advantage is PCR is speedy you can make tons of copies in hours.
Disadvantages are that with PCR cannot substitute for gene cloning in cells when large amounts of a gene are desired. Also occasional errors during PCR replication impose limits on the number of good copies that can be made.
source Campbell and Reece Biology 7th ed
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What are the differences between nested PCR and regular PCR techniques in terms of their applications and advantages in molecular biology research?
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Nested PCR offers increased specificity and sensitivity compared to traditional PCR methods. By using two sets of primers in two separate amplification reactions, nested PCR can reduce non-specific amplification and detect low abundance targets more effectively. This can be particularly useful in cases where the target DNA is present in low concentrations or is closely related to non-target sequences.
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Nested PCR is a variation of regular PCR that involves two rounds of amplification. It is often used when the target DNA is present in low concentrations. Nested PCR can increase the sensitivity and specificity of the test compared to regular PCR. Regular PCR, on the other hand, involves a single round of amplification and is commonly used for routine DNA amplification. Nested PCR is advantageous in detecting low abundance targets, while regular PCR is more suitable for general DNA amplification purposes.
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