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Can vacuum pressure be created inside an HPLC autosampler vial because of over-tightenning of cap or over-filling of vial?
I have worked as a development scientist for over 10 years now. This mythical vacuum has been talked about for many years. It is theoretically possible for a slight vacuum to form if the conditions are just right. For the conditions to apply you need to have:
A sample vial filled beyond the top fill line (partially coming out of the vial with surface tension holding it in) and a very tight cap so that there is almost no air in the vial.
Most autosamplers cannot withdraw more than 100 µL at any given time and the top fill line of a vial is 1.5 mL, the headspace is effectively .2 mL give or take a bit. A 100µL withdraw would create a pressure in the vial of ~ .6 atm until the syringe exits the vial upon which it would equalize. It is very unlikely this would cause an appreciable difference in the injection volume.
If you're looking for sources of error, it's much more likely that there is an issue with the lamp intensity causing the variability. I have seen the vacuum problem before, but it's always in very extenuating circumstances.
What else can I help you with?
How does cardiac output affect blood pressure?
Cardiac output is the amount of blood pumped by the heart in one minute. It directly affects blood pressure because an increase in cardiac output leads to higher blood pressure, while a decrease in cardiac output results in lower blood pressure. This is because the more blood the heart pumps, the more pressure is exerted on the walls of the blood vessels.
Is taking blood pressure in the leg the same as taking it in the arm?
No, taking blood pressure in the leg is not the same as taking it in the arm. Blood pressure is typically measured in the arm because it provides a good reflection of overall systemic blood pressure. Taking blood pressure in the leg can be done in certain medical situations, but the values may differ from those obtained in the arm.
Why do ears pop?
Air pressure. As you increase altitude, air pressure decreases. The pressure within your body then attempts to equalize the pressure and the ear drum membrane is very sensitive to changes in pressure because it thin.
Why does salt affect blood pressure?
Salt affects blood pressure because it can cause the body to retain water, leading to an increase in blood volume. This increased blood volume puts more pressure on the walls of the blood vessels, resulting in higher blood pressure.
Why is it that one ear will not pop when flying or experiencing changes in air pressure?
When flying or experiencing changes in air pressure, one ear may not pop because the Eustachian tube in that ear is not able to equalize the pressure effectively.
How Are Low And High Pressure Different?
It is different because low pressure is on top of high pressure because since on top of low pressure nothing is on top.
Why is boiling dependent on pressure?
Boiling is dependent on pressure because the pressure affects the boiling point of a substance. When the pressure is higher, the boiling point of a substance is also higher, and when the pressure is lower, the boiling point is lower. This is because pressure affects the vapor pressure of the substance, which needs to equal the atmospheric pressure for boiling to occur.
Why is alcohol used in manometer?
Manometer is a device that is being used to measure pressure. It uses alcohol because it has a low vapor pressure.
Why does pressure change change with elevation and depth?
Pressure drops at higher elevations because of the decrease in the weight of air. Under the water, pressure climbs with increasing depth because of the combined weight of the water and that of the atmosphere.
When air is heated air pressure decreases because the molecules?
when air is heated ,air pressure decreases because the molecules are
Why does gas pressure?
Gas release pressure because gases are made of atoms.
Why air pressure is high at low places?
because air pressure is high
Compare air pressure on earth to air pressure on the moon?
The moon has no air pressure because the moon has no air.
Will a tire expand because it heats up after a long drive?
No, but the pressure will increase.No, but the pressure will increase.
Why is their no pressure on the moon?
Because there is no air there.
Why don't people get crushed by atmospheric pressure?
Because your body can only with stand a certain amount of pressure because of your internal organs
Why does Atmospheric pressure exists because air has what?
Atmospheric pressure exists because air has weight. The weight of the air above exerts a force on the air below it, resulting in pressure at the Earth's surface.
