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If you change the pH, the enzyme can become inactive and won't be able anymore to forfill its function.
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Explain the effects of pH on enzyme reaction rates?
A change in pH can denature an enzyme, meaning the reaction would stop.
Which enzyme shows the greatest change in its rate of action with the least change in pH?
The enzyme with lowest pH optimum is pepsin. Pepsin works in the stomach in an acidic environment and aids in digestion.
What is the true relationship between pH and enzyme action?
The enzyme has an optimal point of pH at which the enzyme works best. For example a catalase enzyme works best in a pH of 7. When the pH changes it denatures the enzyme causing it to not be able to react with the substrate.
Identify two factors that could cause enzyme denaturation?
Temperature and pH are the two factors that can cause enzymes to denature.
Temperature and can affect enzyme activity?
Ph level, enzyme concentration, substrate concentration etc
Explain the effects of pH on enzyme reaction rates?
A change in pH can denature an enzyme, meaning the reaction would stop.
Which enzyme shows the greatest change in its rate of action with the least change in pH?
The enzyme with lowest pH optimum is pepsin. Pepsin works in the stomach in an acidic environment and aids in digestion.
What two factors change the efficiency of an enzyme?
pH and temperature.
What is a change in this can denature a protein?
Temperature, pH, organic solvent, mechanical forces
Changing the pH in an enzyme will?
Changing the pH in the environment that an enzyme works in can change how active it will be. Most will be active in a narrow range. Pepsin, a stomach enzyme, will only work at very acid pHs and will become inactive at higher pH than 2.
What changes the rate of a reaction?
* Presence of enzyme * Change in pH * Change in temperature * Change in salt concentration.
How might very low pH affect an enzyme's hydrogen bonds?
A very low pH can break the hydrogen bonds in an enzyme which causes the shape of the enzyme to change shape making the enzyme unable to do it's job. This is called "denaturation" However some enzymes such as pepsin only work in a low pH (pepsin works best in a pH of about 3) so it does depend on the enzyme.
What is the true relationship between pH and enzyme action?
The enzyme has an optimal point of pH at which the enzyme works best. For example a catalase enzyme works best in a pH of 7. When the pH changes it denatures the enzyme causing it to not be able to react with the substrate.
How might amino acid change affect the enzyme?
The pKA of enzyme affects its ionization which could alter enzyme activity. For pH < pKa, the value of vmax is constant and that for pH > pKa, vmax decreases; ie. enzyme activity starts to decline.
What is the effect of excess heat or pH on an enzyme?
it can change the shape of enzymes & active site so the enzyme is unable to work
How are enzymes affected by changes in pH?
A change in pH or and change in temperature will change the shape of the enzyme because its proteins are denatured. Therefore, the enzyme can no longer perform its desired functions because its specific shape has been altered.
How does pH levels effect an enzyme?
All enxymes work at an optimum pH. Changes in pH levels could change the rate at which the enzymes work by denaturing them.
