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in DNA estimation by diphenylamine method, standard calf thymus DNA is digested by perchloric acid, but unkown is not digested by perchloric acid, rather rest protocol of diluting with water and treating with diphenylamine and checking aborbance reamins same can anybody say the reason?
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Which method is preparing DNA for forensic analysis?
The method commonly used for preparing DNA for forensic analysis is called polymerase chain reaction (PCR). PCR amplifies specific regions of DNA so that they can be analyzed in detail. This method allows for small amounts of DNA to be replicated, making it suitable for forensic samples with limited DNA material.
What is a method of making any copies of DNA?
One method of making copies of DNA is through a process called polymerase chain reaction (PCR). In PCR, a DNA template is mixed with primers, nucleotides, and DNA polymerase, and subjected to cycles of heating and cooling to amplify the target DNA region. This results in millions of copies of the DNA target.
What is the reaction mechanism of orcinol in the estimation of RNA?
Furfural cannot be formed from ribosides until the glycosidic linkage has been split; the purine-ribose links of RNA are easily hydrolysed by hot acid, while the pyrimidine-ribose links are much more resistant, and the orcinol method is commonly supposed to determine only the purine-bound ribose of RNA.
Which is a method to prepare DNA for forensic analysis?
One method to prepare DNA for forensic analysis is called DNA extraction. This involves isolating DNA from the sample using various techniques, such as chemical or mechanical disruption of cells, enzymatic digestion, and purification steps to obtain high-quality DNA for analysis.
Dideoxyribonucleotide chain-termination is a method of?
Dideoxyribonucleotide chain-termination is a method used in DNA sequencing to determine the sequence of nucleotides in a DNA molecule. It involves terminating DNA synthesis at specific bases by incorporating dideoxyribonucleotides (ddNTPs) into the growing DNA strand, which lack the 3' hydroxyl group needed for further elongation. This results in a series of fragments of varying lengths that can be separated by size to reveal the DNA sequence.
Why do you use dpa for estimation of DNA other than oricinol?
Only the deoxyribose after heating becomes furfural which then reacts with DPA and produces dark blue coloured compond which can be estimated on the other hand one can say that orcinol does't reacts with deoxyribose similarly DPA does't reacts with ribose
What is front end estimation?
Front end estimation- An estimation method in which the front digits are added or subtracted
What are the methods for estimation of DNA?
Common methods for DNA estimation include spectrophotometry using UV absorbance at 260 nm, fluorometry using DNA-intercalating dyes such as Hoechst or PicoGreen, and quantitative PCR (qPCR) using specific primers to amplify and quantify DNA fragments. Each method has its own advantages and limitations, so selection should be based on the specific requirements of the experiment.
What is a method for math in a estimation?
round or rounding
What is an invasive method of protein estimation?
gand mara
Can one cost estimation method be used in combination with another method?
Yes. True
One cost estimation method can be used in combination with another method.?
Yes. True
Can one cost estimation method can be used in combination with another method?
Yes. True
What are the methods of estimation of reducing sugar?
by comparing the colours or the amount of precipitate
What is the method of estimation using a representative section of a whole?
sampling
What is a method of estimation using a representative section of the whole?
sampling
What is the method of making a rough measurement?
A method of making a rough measurement is: Estimation Answered by :PopTy13
