in DNA estimation by diphenylamine method, standard calf thymus DNA is digested by perchloric acid, but unkown is not digested by perchloric acid, rather rest protocol of diluting with water and treating with diphenylamine and checking aborbance reamins same can anybody say the reason?
The method commonly used for preparing DNA for forensic analysis is called polymerase chain reaction (PCR). PCR amplifies specific regions of DNA so that they can be analyzed in detail. This method allows for small amounts of DNA to be replicated, making it suitable for forensic samples with limited DNA material.
One method of making copies of DNA is through a process called polymerase chain reaction (PCR). In PCR, a DNA template is mixed with primers, nucleotides, and DNA polymerase, and subjected to cycles of heating and cooling to amplify the target DNA region. This results in millions of copies of the DNA target.
Furfural cannot be formed from ribosides until the glycosidic linkage has been split; the purine-ribose links of RNA are easily hydrolysed by hot acid, while the pyrimidine-ribose links are much more resistant, and the orcinol method is commonly supposed to determine only the purine-bound ribose of RNA.
One method to prepare DNA for forensic analysis is called DNA extraction. This involves isolating DNA from the sample using various techniques, such as chemical or mechanical disruption of cells, enzymatic digestion, and purification steps to obtain high-quality DNA for analysis.
Dideoxyribonucleotide chain-termination is a method used in DNA sequencing to determine the sequence of nucleotides in a DNA molecule. It involves terminating DNA synthesis at specific bases by incorporating dideoxyribonucleotides (ddNTPs) into the growing DNA strand, which lack the 3' hydroxyl group needed for further elongation. This results in a series of fragments of varying lengths that can be separated by size to reveal the DNA sequence.
Only the deoxyribose after heating becomes furfural which then reacts with DPA and produces dark blue coloured compond which can be estimated on the other hand one can say that orcinol does't reacts with deoxyribose similarly DPA does't reacts with ribose
Front end estimation- An estimation method in which the front digits are added or subtracted
Common methods for DNA estimation include spectrophotometry using UV absorbance at 260 nm, fluorometry using DNA-intercalating dyes such as Hoechst or PicoGreen, and quantitative PCR (qPCR) using specific primers to amplify and quantify DNA fragments. Each method has its own advantages and limitations, so selection should be based on the specific requirements of the experiment.
round or rounding
gand mara
Yes. True
Yes. True
Yes. True
by comparing the colours or the amount of precipitate
sampling
sampling
A method of making a rough measurement is: Estimation Answered by :PopTy13