0
Furfural cannot be formed from ribosides until the glycosidic
linkage has been split; the purine-ribose links of RNA are easily
hydrolysed by hot acid, while the pyrimidine-ribose links are much more
resistant, and the orcinol method is commonly supposed to determine only
the purine-bound ribose of RNA.
What else can I help you with?
What does bials orcinol determine in nucleic acid?
Bial's test is used to determine the presence of a pentose sugar. For example, the sugar ribose would turn green (positive) and the sugar glucose would turn brown or yellow (negative). Nucleic acids (DNA and RNA) both contain a suger. RNA contains ribose, so it should have a positive orcinol test. DNA contains deoxyribose, which should have a weak reaction, yielding what appears to be a negative result.
Does PCR use RNA primers in its process?
No, PCR (polymerase chain reaction) uses DNA primers, not RNA primers, in its process.
Why is RNA interferon discovery important?
RNA carries out several important roles. There are 3 main types of RNA, messenger RNA (mRNA), ribosomal RNA (rRNA) and transfer RNA (tRNA). mRNA carries a copy of the instructions for creating proteins from the DNA in the nucleus to the ribosomes for translation. rRNA makes up part of the ribosomes. tRNA carries amino acids (the building blocks of proteins) to the ribosomes
What is synthesized by RNA polymerase?
Polymerases are the enzymes that replicate and build nucleic acids. DNA polymerases synthesize DNA, RNA polymerases synthesize RNA. Purified polymerases are essential to carrying out the PCR reaction.
Why is RNA polymerase a good name for that enzyme?
RNA polymerase is a good name for the enzyme because it accurately describes its function – it is an enzyme that synthesizes RNA molecules from a DNA template by linking nucleotides together in a polymerization reaction. The name "polymerase" indicates that it is involved in polymerization, while "RNA" specifies the type of nucleic acid molecule being synthesized.
What is Principle behind using orcinol reagent in extraction of RNA from yeast?
The method depends on conversion of the pentose, ribose in the presence of hot acid to furfural which then reacts with orcinol to yield a green color. The color formed largely depends on the concentration of HCl, ferric chloride, orcinol, the time of heating at 100°C etc up to certain maxima.
What does bials orcinol determine in nucleic acid?
Bial's test is used to determine the presence of a pentose sugar. For example, the sugar ribose would turn green (positive) and the sugar glucose would turn brown or yellow (negative). Nucleic acids (DNA and RNA) both contain a suger. RNA contains ribose, so it should have a positive orcinol test. DNA contains deoxyribose, which should have a weak reaction, yielding what appears to be a negative result.
What is the theoretical result with orcinol reagent?
When orcinol reagent is added to a solution containing pentoses, a chromophore is formed that absorbs light at 660 nm. This allows for the detection and quantification of pentoses, such as ribose, in a sample. The intensity of the color generated is directly proportional to the concentration of pentoses present.
What sugar does RNA have that is different from the sugar in the morning?
Which mechanism explains the phenomenon
Is RNA a thought to be the oldest and most primitive coding mechanism for life?
Yes it is the oldest and most primitive coding mechanism
How could RNA have become involved in the mechanism for protein translation?
natural selection favored RNA molecules that synthesized catalytic proteins
What is the indicator for nucleic acids?
Detection of pentose/ribose sugar in RNA (by performing Bial's test) can be done with Orcinol reagent (0.3% orcinol solution prepared in concentrated HCl). This method requires the following reagents 1. Orcinol reagent : 6% orcinol in 95% ethanol. 2. Acid reagent : 100 ml. of conc. HCl, 0.5 ml. of 10% FeCl3, 10H2O is added (to an aqueous RNA solution (1 mg. per ml.)) Detection of deoxyribose/deoxypentose sugar in DNA can be identified chemically with the Dische diphenylamine test. This method requires the following reagents one gram of purified diphenylamineis dissolved in acetic acid and volume made to 100 ml. with acetic acid. Afterwards, 2.75 ml. of conc. H2SO4 is added for stablization. Schiff's reagent is another sensitive means , and can be used in a method to demonstrate deoxyribosenucleic acid (DNA) specifically (of detecting aldehydes), in contrast to unstained ribosenucleic acid (RNA). This method is the nucleal reaction of Feulgen and Rossenbeck (called the Feulgen stain or reaction). It is usually done with pararosaniline Schiff solution (pseudo-Schiff reagents), but it works well with some others, including the fluorescent acriflavine solution. This method requires the following reagents Hydrochloric acid, 1Normal Schiff's reagent (made from pararosanilin treated with sulphurous acid) Light green, 1% aqueous (can be replaced with Fast green FCF)
Antitermination of RNA synthesis is a major mechanism of regulation in?
Antitermination of RNA synthesis is a major mechanism of regulation in prokaryotic gene expression. It allows transcription to continue past termination signals in certain conditions, enabling the production of full-length transcripts. This mechanism often involves regulatory proteins that interact with mRNA secondary structures to modulate RNA polymerase activity.
Does PCR use RNA primers in its process?
No, PCR (polymerase chain reaction) uses DNA primers, not RNA primers, in its process.
Why is RNA interferon discovery important?
RNA carries out several important roles. There are 3 main types of RNA, messenger RNA (mRNA), ribosomal RNA (rRNA) and transfer RNA (tRNA). mRNA carries a copy of the instructions for creating proteins from the DNA in the nucleus to the ribosomes for translation. rRNA makes up part of the ribosomes. tRNA carries amino acids (the building blocks of proteins) to the ribosomes
During splicing which molecular component of the spliceosome catalyzes the excision reaction?
The molecular component of the spliceosome that catalyzes the excision reaction during splicing is the RNA component known as the catalytic RNA or ribozyme. It is responsible for the cleavage and ligation of the precursor messenger RNA (mRNA) molecules, ensuring the removal of introns and joining of exons to generate mature mRNA.
What is the name of the chemical reaction when a strand of messenger RNA is formed from individual nucleotides?
The process of forming a strand of messenger RNA from individual nucleotides is called transcription. During transcription, an enzyme called RNA polymerase helps to assemble the nucleotides in the correct sequence based on the DNA template.
