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What does bial-orcinol reagent composed of?
300 mg of orcinol in 100 ml of conc. HCl and 0.25 ml of ferric chloride solution (10g / 100ml ).
What is Principle behind using orcinol reagent in extraction of RNA from yeast?
The method depends on conversion of the pentose, ribose in the presence of hot acid to furfural which then reacts with orcinol to yield a green color. The color formed largely depends on the concentration of HCl, ferric chloride, orcinol, the time of heating at 100°C etc up to certain maxima.
Where does the theoretical yield come from the amount of product you get in a reaction or the amount of product that could be made from the limiting reagent?
The amount of product you get in a reaction
Indication of a negative result with Bial's Reagent?
A negative result with Bial's reagent will show no color change, indicating the absence of pentoses in the test sample. This can be confirmed by the lack of a green color formation, which is characteristic of a positive result when pentoses are present.
What color is produced of hydrolyzed DNA in bial's test?
Hydrolyzed DNA in Bial's test produces a blue-green color due to the reaction of deoxyribose sugars with orcinol reagent and sulfuric acid. This color change indicates the presence of pentoses in the sample.
What does bial-orcinol reagent composed of?
300 mg of orcinol in 100 ml of conc. HCl and 0.25 ml of ferric chloride solution (10g / 100ml ).
What is Principle behind using orcinol reagent in extraction of RNA from yeast?
The method depends on conversion of the pentose, ribose in the presence of hot acid to furfural which then reacts with orcinol to yield a green color. The color formed largely depends on the concentration of HCl, ferric chloride, orcinol, the time of heating at 100°C etc up to certain maxima.
Positive result for bial-orcinol test?
bluish color if pentose is present.
How do you calculate the percent yield of an element in a reaction?
The actual yield is given within the worded problem. The theoretical yield however is to be found. In order to calculate the theoretical yield, you need to convert the mass of the limiting reagent (LR) to the mass of the reagent in excess (ER). To find the limiting reagent, you need to first convert the mass of the limiting reagent to the mass of the reagent in excess (to find the theoretical yield)A. Convert mass of limiting reagent to mass reagent in excess (mass --> mass); in other words, find the mass of the reagent consumed.1. Multiply the mass of the limiting reagent by the # molecules of the reagent in excess and its molar mass.2. Divide the above by the # of molecules LR which is multiplied by its molar mass.Mass LR x # molecules ER x molar mass ER-- # molecules LR x molar mass LR3. Subtract the original mass of reagent in excess and the consumed reagent in excess.Original mass Reagent in Excess - Consumed Reagent in Excess= Reagent leftover/unused (theoretical yield).B. Find the percent yield of the product.--- Actual YieldTheoretical Yield x 100%
What steps are needed to determine the amount of excess reagent in a chemical reaction?
To determine the amount of excess reagent in a chemical reaction, first calculate theoretical values for your reaction to get an estimation of how much of your excess reagent will be left once the limiting reagent is used. Then run the actual experiment and measure!
What does bials orcinol determine in nucleic acid?
Bial's test is used to determine the presence of a pentose sugar. For example, the sugar ribose would turn green (positive) and the sugar glucose would turn brown or yellow (negative). Nucleic acids (DNA and RNA) both contain a suger. RNA contains ribose, so it should have a positive orcinol test. DNA contains deoxyribose, which should have a weak reaction, yielding what appears to be a negative result.
What does bial's orcinol test determine in samples?
Bial's orcinol test is used to detect the presence of pentoses (such as ribose and deoxyribose) in samples. When pentoses react with orcinol in the test, a blue-green color is produced. This test is commonly utilized in the analysis of carbohydrates, nucleic acids, and other biological molecules.
What is the indicator for nucleic acids?
Detection of pentose/ribose sugar in RNA (by performing Bial's test) can be done with Orcinol reagent (0.3% orcinol solution prepared in concentrated HCl). This method requires the following reagents 1. Orcinol reagent : 6% orcinol in 95% ethanol. 2. Acid reagent : 100 ml. of conc. HCl, 0.5 ml. of 10% FeCl3, 10H2O is added (to an aqueous RNA solution (1 mg. per ml.)) Detection of deoxyribose/deoxypentose sugar in DNA can be identified chemically with the Dische diphenylamine test. This method requires the following reagents one gram of purified diphenylamineis dissolved in acetic acid and volume made to 100 ml. with acetic acid. Afterwards, 2.75 ml. of conc. H2SO4 is added for stablization. Schiff's reagent is another sensitive means , and can be used in a method to demonstrate deoxyribosenucleic acid (DNA) specifically (of detecting aldehydes), in contrast to unstained ribosenucleic acid (RNA). This method is the nucleal reaction of Feulgen and Rossenbeck (called the Feulgen stain or reaction). It is usually done with pararosaniline Schiff solution (pseudo-Schiff reagents), but it works well with some others, including the fluorescent acriflavine solution. This method requires the following reagents Hydrochloric acid, 1Normal Schiff's reagent (made from pararosanilin treated with sulphurous acid) Light green, 1% aqueous (can be replaced with Fast green FCF)
Where does the theoretical yield come from the amount of product you get in a reaction or the amount of product that could be made from the limiting reagent?
The amount of product you get in a reaction
Indication of a negative result with Bial's Reagent?
A negative result with Bial's reagent will show no color change, indicating the absence of pentoses in the test sample. This can be confirmed by the lack of a green color formation, which is characteristic of a positive result when pentoses are present.
What color is produced of hydrolyzed DNA in bial's test?
Hydrolyzed DNA in Bial's test produces a blue-green color due to the reaction of deoxyribose sugars with orcinol reagent and sulfuric acid. This color change indicates the presence of pentoses in the sample.
How does a limiting reagent affect how much product is formed?
The amount of product will be limited by the number of moles of the limitin... reagent.
