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The positive result- the resulting blue solution, indicates that the test carbohydrates is a pentose(ribose for example). The blue color is due to the iron content of ferric chloride.

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14y ago
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15y ago

A test for pentoses with orcinol.

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Q: What does bial's orcinol test determine in samples?
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What does bials orcinol determine in nucleic acid?

Bial's test is used to determine the presence of a pentose sugar. For example, the sugar ribose would turn green (positive) and the sugar glucose would turn brown or yellow (negative). Nucleic acids (DNA and RNA) both contain a suger. RNA contains ribose, so it should have a positive orcinol test. DNA contains deoxyribose, which should have a weak reaction, yielding what appears to be a negative result.


What is the discussion of bials test?

discussion abut bial test ??


What is the Chemical equation of bials test?

Dehydration of monosaccharide to form furfural http://www.harpercollege.edu/tm-ps/chm/100/dgodambe/thedisk/carbo/bial/bials.htm Formation of furfural/orcinol complex http://61.19.145.8/student/m5year2006-2/502/group08/rooms5.html


Positive result for bial-orcinol test?

bluish color if pentose is present.


What is the reaction mechanism of orcinol in the estimation of RNA?

Furfural cannot be formed from ribosides until the glycosidic linkage has been split; the purine-ribose links of RNA are easily hydrolysed by hot acid, while the pyrimidine-ribose links are much more resistant, and the orcinol method is commonly supposed to determine only the purine-bound ribose of RNA.


How would you be able to determine from where nutrient influx comes?

You can test the nutrient influx by using a water test. You will determine the historical patterns and then collect water samples from the surface and subsurface layers along the estuaries.


How do you determine if methane and other organics are leaching out of a landfill?

Monitor the area using a methane meter or explosimeter, and test samples of seepage and water run-off.


Is Paired samples T-test an example of nonparametric tests?

A paired samples t-test is an example of parametric (not nonparametric) tests.


Which type of diagnostic test should you do to determine drug and toxity?

Urine, saliva, stomach cotents, and blood samples can be used to determine both, depending on the drug itself as well as how much of the drug is still preset in the body.


What are the methods used for identifying motility?

There are two basic method to determine motility in a bacterial sample. These samples have similar biochemical identifiers. The first test is a simple drop test. The second method employs a motility medium


What are test tubes called?

A group of test tubes are called samples.


What is a presumptive test?

Presumptive test to confirmation of coliforms bacteria in the samples