0
Yes, DNA denatures at the A-T region first because there are only 2 hydrogen bonds between A and T instead of the three hydrogen bonds between C and G. This means the C-G pairs will be held together more tightly.
Wiki User
∙ 12y ago
Add your answer:
Earn +20 pts
What is the function of formamide loading buffer?
To denature DNA
How does formamide denature DNA?
Through the magic of hydrogen bonding
Why is cold ethanol better than room temperaure for DNA isolation?
DNA does not dissolve on Alcohols. It is better to store it in cold temp. for it not to denature
What is the role chelex-100 in DNA extraction?
Chelex is there to simply denature the Double stranded DNA and convert it to single strand DNA so the primers can attatch to it during PCR.
How does hot water work to release DNA inside the cell nuclei?
The cells are incubated in a hot water bath in order to denature the cytoplasmic enzymes which break apart DNA.
What is the function of formamide loading buffer?
To denature DNA
What is the first step in the polymerase chain reaction?
It allows the biologists to make many copies of a dna strand...
Why must DNA fragments be exposed to an alkaline solution before hybridization?
To denature the DNA
How does formamide denature DNA?
Through the magic of hydrogen bonding
What can denature DNA?
by heating above certain temprature eg.90 or 100 degree celcius or by treting with strong alkali or strong acid you can denature your DNA *Actually, you can denature DNA in water if you wanted to. Basically any polar solvent will denature DNA because it has a negatively charged sugar-phosphate backbone. Mutagens can also influence DNA although it isn't exactly denaturing it. So can high energy light, like UV or all kinds of radiation. This, too, isn't denaturing though.
Why is cold ethanol better than room temperaure for DNA isolation?
DNA does not dissolve on Alcohols. It is better to store it in cold temp. for it not to denature
What is the role chelex-100 in DNA extraction?
Chelex is there to simply denature the Double stranded DNA and convert it to single strand DNA so the primers can attatch to it during PCR.
Does boiling denature cells?
Boiling can allow for the extraction of DNA.
Can you tamper with DNA?
Not directly. Radiation can cause mutations in DNA. Excess heat (as in the case of a fever) can denature (destroy) the DNA sequence as well as other proteins which will usually result in cell death.
Are sense regions for of DNA introns and nonsense regions exons?
yup
How does hot water work to release DNA inside the cell nuclei?
The cells are incubated in a hot water bath in order to denature the cytoplasmic enzymes which break apart DNA.
What are four factors or substances that can denature proteins?
Temperature (high), Research Gel electrophresis (method for breaking up DNA)
