0
What else can I help you with?
Why is the lac operon said to be an inducible operon?
The lac operon is considered an inducible operon because it is activated in the presence of lactose as an inducer molecule. When lactose is present, it binds to the repressor protein, causing it to be released from the operator region and allowing RNA polymerase to transcribe the genes involved in lactose metabolism.
What factors affect the rate of enzyme activity?
Factors that affect the rate of enzyme activity include temperature, pH, substrate concentration, and enzyme concentration. Temperature and pH can alter the shape of the enzyme, affecting its ability to bind to the substrate. Changes in substrate and enzyme concentration can affect the frequency of enzyme-substrate collisions, which impacts the rate of reaction.
What happens to the vmax when a competitive reversible inhibitor is added to an enzyme?
The Vmax of the enzyme will remain constant in the presence of a competitive reversible inhibitor. However, the apparent Km will increase as the inhibitor competes with the substrate for binding to the active site of the enzyme, leading to a decrease in enzyme-substrate affinity.
What is the substrate for the Indole test?
The indole test is a biochemical test performed on bacterial species to determine the ability of the organism to split indole from the amino acid tryptophan. This division is performed by a chain of a number of different intracellular enzymes, a system generally referred to as "tryptophanase."
How is lac operon different from trp operon?
1. in lac operon; gene activity is induced when lactose is present in the medium, whereas in case of trp operon, repression of the gene activity takes place in presence of tryptophan in the medium. 2. lac operon spans about 4-6kb...whereas trp operon spans abut 7kb. 3. lac operon helps in the breakdown of lactose into glucose and galactose, to generate energy( catabolic pathway ) in case of trp operon, it helps in the synthesis of enzymes required for the formation of the amino acid Tryptophan( anabolic pathway ). 4. lac operon is an example of positive regulation ; and trp operon is an example of negative regulation. Trp operon is also regulated by other mechanism called attenuation while no such even occurs in lac operon . .
Turning genes on in response to the presence of substrate is called?
Induction
What affects enzymatic speed?
Enzymatic speed can be affected by factors such as temperature, pH, substrate concentration, enzyme concentration, and the presence of inhibitors or activators. These factors can alter the enzyme's ability to bind to the substrate and catalyze the reaction.
Why is the lac operon said to be an inducible operon?
The lac operon is considered an inducible operon because it is activated in the presence of lactose as an inducer molecule. When lactose is present, it binds to the repressor protein, causing it to be released from the operator region and allowing RNA polymerase to transcribe the genes involved in lactose metabolism.
What factors affect the rate of enzyme activity?
Factors that affect the rate of enzyme activity include temperature, pH, substrate concentration, and enzyme concentration. Temperature and pH can alter the shape of the enzyme, affecting its ability to bind to the substrate. Changes in substrate and enzyme concentration can affect the frequency of enzyme-substrate collisions, which impacts the rate of reaction.
What can effect how the enzyme and substrate come together?
Factors such as temperature, pH, substrate concentration, and the presence of inhibitors or activators can affect how enzymes and substrates come together. Changes in these factors can alter the shape and activity of enzymes, impacting their ability to bind with substrates and catalyze reactions.
What is the advantage of enzymes not being used up in reactions?
Let say enzyme 1 has a shape of A. When it encounters a solute particle of shape A', enzyme jumps on the particle so to speak changing its shape to A'' and back to A' in less than nano second and when that shape change happens the solute particle becomes highly unstable and now can react with other solute particles and hence carry out the reaction. Enzyme jumps on substrate --> substrate unstable --> enzyme jumps back out of substrate --> unstable substrate reacts with another substrate.
What happens to the vmax when a competitive reversible inhibitor is added to an enzyme?
The Vmax of the enzyme will remain constant in the presence of a competitive reversible inhibitor. However, the apparent Km will increase as the inhibitor competes with the substrate for binding to the active site of the enzyme, leading to a decrease in enzyme-substrate affinity.
What is the substrate for the Indole test?
The indole test is a biochemical test performed on bacterial species to determine the ability of the organism to split indole from the amino acid tryptophan. This division is performed by a chain of a number of different intracellular enzymes, a system generally referred to as "tryptophanase."
What is the Vmax in the presence of the inhibitor?
Vmax, or maximum velocity, refers to the maximum rate at which an enzyme can catalyze a reaction when fully saturated with substrate. In the presence of a competitive inhibitor, Vmax remains unchanged because the inhibitor does not affect the enzyme's ability to catalyze the reaction at high substrate concentrations; it only increases the apparent Km. However, for non-competitive inhibitors, Vmax is reduced because the inhibitor affects the enzyme's function regardless of substrate concentration. Thus, the specific effect on Vmax depends on the type of inhibitor present.
What is the advantage of the presence of on cell membranes?
the membrane has a greater surface area for an increased rate of diffusion
How is lac operon different from trp operon?
1. in lac operon; gene activity is induced when lactose is present in the medium, whereas in case of trp operon, repression of the gene activity takes place in presence of tryptophan in the medium. 2. lac operon spans about 4-6kb...whereas trp operon spans abut 7kb. 3. lac operon helps in the breakdown of lactose into glucose and galactose, to generate energy( catabolic pathway ) in case of trp operon, it helps in the synthesis of enzymes required for the formation of the amino acid Tryptophan( anabolic pathway ). 4. lac operon is an example of positive regulation ; and trp operon is an example of negative regulation. Trp operon is also regulated by other mechanism called attenuation while no such even occurs in lac operon . .
How does the presence of competitive inhibitors impact the maximum reaction rate (Vmax) of an enzyme?
Competitive inhibitors decrease the maximum reaction rate (Vmax) of an enzyme by competing with the substrate for the enzyme's active site, which reduces the efficiency of the enzyme-substrate complex formation and slows down the rate of the reaction.
