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laminar flow
Smooth, orderly movement of a fluid, in which there is no turbulence, and any given subcurrent moves more or less in parallel with any other nearby subcurrent. Laminar flow is common in viscous fluids, especially those moving at low velocities.
Its used to avoid the spreading of fumes and gases when conducting an experiment resulting in a gaseous reaction.
What else can I help you with?
What are the different measures taken to prevent contamination of culture media?
Some measures to prevent contamination of culture media include proper sterilization techniques, maintaining a clean work area, using aseptic techniques, handling cultures in a laminar flow hood, and promptly closing containers after use. Regular monitoring and quality control checks should also be performed to ensure the media remains contamination-free.
How do you do the cleaning of LAF?
Cleaning of LAF is done to prevent the growth of unwanted microbes in culture made for growth of required microbe. this is why it's done the how is first using sterile water for irrigation if you have a vertical LAF hood (most hospitals have these) the HEPA filter will be at the top . you start at the back using overlaping wipes DO NOT clean the hood as if you were cleaning windows!!!! next you use 70% alcohol the order it's cleaned in is Back Side Bar Side Bottom ... the best way to remember the order in which you have to clean is a bunch of BS (back side bar side bottom - BSBSB..lol)
What is a 'nun's hood' when used to describe a body part related to the female anatomy?
The term "nun's hood" is a colloquial term for the clitoral hood, which is a fold of skin that covers and protects the clitoris. The clitoral hood is analogous to the male foreskin and serves to provide sensitivity and protection to the clitoris. It can also vary in size and shape among individuals.
Lists of aseptic technique you used in lab?
There are different types of Aseptic Techniques such as uphold of sterilastion. This is when you insure that the sterile sections in labs are kept sterile, by using sterile equiptment (such as gloves, etc) and washing hands for at least 2-5mins before entering sterile environments. Another technique is when dealing with sterile equiptment, not only do you use gloves, but ensure that you use the procedure of handeling equiptment in a certain way. Tilt the testube to the side for example, when you are screwing and unscrewing the lid of a container, using the side of your hands, not touching the rim with yor fingers etc. This should help a bit :)
Name of laboratory appratus?
Microscopes - for viewing small objects >antibodies - to highlight certain areas of the cell >dyes - to highlight certain areas of the cell >fluorescent proteins - to highlight certain areas of the cell (usually proteins) Laminar flow hood - prevent leaking of microbes from the hood Plates/dishes/tubes - to culture cells (grow them) Gels and blots - to determine the presence of various genes/proteins Incubators - to maintain healthy temperatures to grow cells Heaters/coolers - to assist in PCR reactions Freezer - to store bilogical samples Centrifuge - to separate biological components via weight Mass Spectrometer - to determine to components of a solution X-ray crystallography - to determine the shape of a protein Radioactive isotopes - to track the changes of certain substrates in a biological reaction Colorimeter - to quantitatively determine the presence of various substances Bunsen burner+alcohol - to help maintain sterility and many many more
What are the differences between horizontal and vertical laminar flow hoods?
In a horizontal laminar flow hood, the laminar air flows towards the outside (i.e. towards the worker). There is hence no protection to the worker and it is not suitable for cytotoxic drug preparation. In a vertical laminar flow hood, the laminar air flows downwards from the top, providing more protection for the workers. Vertical laminar flow hoods are hence suitable for preparing cytotoxic drugs.
Why is no obstruction important for air flow techniques?
The laminar flow hood depends on the laminar air flow to sweep away pathogens from the hood. If there is obstruction, the laminar air flow pattern will be disrupted. The laminar air flow will then change to turbulent air flow.
When working in a horizontal laminar air flow hood you must work at least how many inches in from the outside edge of the work surface?
When working in a horizontal laminar flow hood, you should work at least 6 inches in from the outside edge of the work surface. This is to ensure that the laminar airflow remains undisturbed and effective in maintaining a clean work environment for your experiments.
What is the Difference between vertical hood and horizontal hood?
In the horizontal hood the air is blown across the work surface toward the operator (air flow from back to front) and in the vertical hood the air is blown down onto the work surface (air flow from top to bottom).
What is the horizontal laminar flow hood used for?
Its used to avoid the spreading of fumes and gases when conducting an experiment resulting in a gaseous reaction.
Function of laminar flow hood?
A laminar flow hood is used to create a sterile work environment by directing filtered air in a horizontal, unidirectional flow. It is commonly used in laboratories and clean rooms to prevent contamination of sensitive materials, equipment, or samples during procedures such as cell culture, microbiological work, or pharmaceutical compounding.
Which solution is recommended for cleaning a laminar flow hood?
isopropyl alcohol
What should be the size of HEPA filter for laminar flow hood?
0.2 Micron
What is the safety of laminar flow?
A laminar flow prevents the inappropriate circulation of air within a hood where cells or bacteria are being cultured. This prevents contamination and also insures the safety of the worker
How a laminar flow hood must be cleaned?
A laminar flow hood should be cleaned regularly using a disinfectant that is compatible with the materials used in the hood. Wipe down all surfaces, including the HEPA filter, shelves, and walls, with the disinfectant. Be sure to follow the manufacturer's cleaning instructions to maintain the hood's effectiveness.
What is the underlying mechanism of a laminar flow hood in microbiology?
The underlying mechanism of a laminar flow hood in any application, including microbiology is that a roughly non-turbulent flow of air is more effective at preventing cross contamination of materials in the hood and having that air go through a high efficiency filter first is effective in preventing contamination by microbes and other items from outside the hood.
How often are filters in a laminar flow hood changed?
Filters in a laminar flow hood are typically changed every 6-12 months, depending on the frequency of use and the cleanliness of the environment in which the hood is located. It is important to follow manufacturer guidelines and conduct regular inspections to ensure optimal performance and safety.
