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During transcription, RNA polymerase binds to a specific region of the DNA called the promoter. It then unwinds the DNA double helix by breaking the hydrogen bonds between the base pairs, allowing one of the DNA strands to serve as a template for the synthesis of RNA. This process is facilitated by the enzyme's helicase activity, which helps separate the two DNA strands and create a single-stranded template for RNA synthesis.
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How does RNA polymerase unwind DNA during transcription?
During transcription, RNA polymerase binds to a specific region of DNA and unwinds the double helix structure by breaking the hydrogen bonds between the base pairs. This allows the enzyme to access the DNA template strand and synthesize a complementary RNA strand.
How does the transcription helicase enzyme facilitate the unwinding of DNA during the transcription process?
The transcription helicase enzyme helps to unwind the double-stranded DNA by breaking the hydrogen bonds between the two strands. This allows the RNA polymerase enzyme to access the DNA template and create a complementary RNA strand during the transcription process.
What is the function of sigma factor in transcription?
when RNA Polymerase meet the correct promoter(TATA box), it will bind at that region and then sigma factor will also bind to the RNA Polymerase.once ATP give energy, sigma factor will dissoiates from RNA Polymerase and the enzyme start to unwind the double helix
What does Transcription in eukaryotes require in addition to RNA polymerase?
Transcription in eukaryotes requires additional transcription factors, which are proteins that help RNA polymerase recognize the promoter region of a gene, initiate transcription, and regulate gene expression. These transcription factors are essential for the accurate and efficient transcription of genes in eukaryotic cells.
What must happen to DNA strands before transcription can begin?
The DNA strands must separate or unwind to expose the specific gene that is going to be transcribed. This process is facilitated by enzymes that help unzip the double-stranded DNA. Once the DNA is unwound, RNA polymerase can then bind to the DNA and initiate transcription.
How does RNA polymerase unwind DNA during transcription?
During transcription, RNA polymerase binds to a specific region of DNA and unwinds the double helix structure by breaking the hydrogen bonds between the base pairs. This allows the enzyme to access the DNA template strand and synthesize a complementary RNA strand.
How does the transcription helicase enzyme facilitate the unwinding of DNA during the transcription process?
The transcription helicase enzyme helps to unwind the double-stranded DNA by breaking the hydrogen bonds between the two strands. This allows the RNA polymerase enzyme to access the DNA template and create a complementary RNA strand during the transcription process.
What is the function of sigma factor in transcription?
when RNA Polymerase meet the correct promoter(TATA box), it will bind at that region and then sigma factor will also bind to the RNA Polymerase.once ATP give energy, sigma factor will dissoiates from RNA Polymerase and the enzyme start to unwind the double helix
What does Transcription in eukaryotes require in addition to RNA polymerase?
Transcription in eukaryotes requires additional transcription factors, which are proteins that help RNA polymerase recognize the promoter region of a gene, initiate transcription, and regulate gene expression. These transcription factors are essential for the accurate and efficient transcription of genes in eukaryotic cells.
What must happen to DNA strands before transcription can begin?
The DNA strands must separate or unwind to expose the specific gene that is going to be transcribed. This process is facilitated by enzymes that help unzip the double-stranded DNA. Once the DNA is unwound, RNA polymerase can then bind to the DNA and initiate transcription.
What event occurs directly after RNA polymerase recognizes the transcription start of a gene?
Initiation of transcription occurs, involving the unwinding of the DNA helix and the binding of RNA polymerase to the promoter region of the gene. Transcription factors and other regulatory proteins aid in the initiation process.
What must happen to a DNA molecule before RNA polymerase can make RNA?
The DNA molecule must first unwind and separate into two strands. This process is called transcription, during which RNA polymerase can then read and transcribe one of the DNA strands to synthesize RNA.
The correct sequence of the events of transcription is?
RNA polymerase reaches the beginning of a gene.
What process is the first step in making a protein from DNA instructions?
The first step in creating a protein is transcription - the copying of the code from DNA to mRNA. (The section of DNA must unwind before this can happen).
Where does RNA polymerase bind?
RNA polymerase bind specific regions of DNA called promoters. The RNA polymerase holoenzyme is guided to promoters by interactions between members of the holoenyzme and specific DNA sequences such as the TATA box.
What must happen to the helix of DNA for transcription to occur?
For transcription to occur, the double helix structure of DNA must unwind and separate at the promoter region of the gene being transcribed. This unwinding exposes the template strand of the DNA, allowing RNA polymerase to synthesize a complementary RNA strand by adding ribonucleotides according to the sequence of the DNA template. The DNA helix reforms after the transcription process is complete.
What is initiation in transcription?
So in Transcription there are three main steps: Initiation, elongation and termination. The one I'm focusing on is Initiation. In eukaryote, proteins called transcription factors mediate the initiation of transcription by RNA Polymerse II. A eukaryotic promoter commonly includes a TATA box, a nucleotide sequence containing "Thymine-Adenine-thymine-adenine", about 25 nucleotides upstream from the transcriptional start point.
