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What is the easiest way to get a desired cell concentration from a flask?
The easiest way to achieve a desired cell concentration in a flask is to calculate the volume of cells needed based on the initial concentration and desired final concentration, then add or remove media accordingly to adjust the cell concentration. Regularly checking the cell concentration using a hemocytometer or cell counter can help ensure the desired concentration is maintained.
Why heterotrops prefer complex media?
Complex media are preferable for routine cultivation of microorganisms than chemically defined media because it provides highly enriched sources such as plant or animal extracts, vitamins, minerals, protein, sugar, and or blood to the organism, supplying them specific nutritional substances needed for their growth. Chemically defined media on the other hand does not supply such a vast abundance of nutritional substances.
If sim medium was used for motility determination for Proteus vulgaris what noticeable change to the medium will be observed?
A noticeable change in the medium due to Proteus vulgaris motility would be the presence of diffuse growth radiating from the point of inoculation, resulting in a fuzzy or feathery appearance on the surface of the agar. This motility pattern is characteristic of Proteus species, which exhibit swarming motility on semisolid media like SIM medium.
What are the different types of media used in microbiology for cultivating and studying microorganisms?
The different types of media used in microbiology for cultivating and studying microorganisms include agar plates, broth media, and specialized media such as selective and differential media. Agar plates provide a solid surface for microbial growth, while broth media are liquid-based for growing microorganisms. Selective media encourage the growth of specific types of microorganisms, while differential media help distinguish between different types of microorganisms based on their characteristics.
Compare simple media with differential media?
Simple media provides essential nutrients for the growth of most microorganisms, while differential media contains specific ingredients that allow for the differentiation of different types of microorganisms based on their metabolic activities or biochemical properties. Differential media can help distinguish between different species or strains of bacteria, while simple media is more general and supports the growth of a wide variety of microorganisms without differentiation.
What is a semisolid medium?
A semisolid medium is a type of growth medium used in microbiology that has a consistency between solid and liquid. It contains a lower concentration of agar compared to solid media, allowing for the growth of organisms that require a more viscous environment to spread and move within the medium. Semisolid media are commonly used for motility and diffusion assays.
What is the concentration of bacteriological agar normally used in making solid media?
Agar is added in a concentration of 1.5% to solidify liquid media. Although there are different concentrations of agar used to create different conditions such as in Sabouraud's Agar and semisolid agar.
The three physical forms of laboratory media are?
solid, semisolid, liquid
Why are semisolid media sometimes preferred over techniques for evaluating bacterial motility?
Because it does not inhibit bacteria form "swimming" through the medium.
What is a media concentration?
Media concentration refers to the ownership of a significant portion of media outlets by a small number of companies or individuals. This concentration of ownership can impact diversity of viewpoints, media content, and competition within the media industry. It raises concerns about potential biases, limited access to information, and reduced media pluralism.
What media are use to determine motility and growth patterns of bacteria?
Common media used to determine motility and growth patterns of bacteria include agar-based media such as nutrient agar, motility agar, or semisolid agar. These media allow for observing colony morphology, motility (through diffusion in semisolid agar), and growth patterns (e.g., pellicle formation). Additionally, specialized media like SIM (sulfide-indole-motility) agar can be used to detect hydrogen sulfide production, indole production, and motility of specific bacteria.
Does brand building through digital marketing need to be radically different by conventional media?
Yes, brand building through digital marketing is radically different from conventional media as through digital marketing you can brand your product or services worldwide and through conventional media you can promote you business through limited medium and limited place.
Does abstract sculpture differ from abstract painting?
no, just the media that is used
How is storage media differ from RAM in terms of volatility?
Because of bananas
The trend in ownership of mass media in the US has been toward?
Greater concentration of ownership
What is an example of media concentration?
An example of media concentration is when a few large conglomerates or corporations own multiple media outlets, such as TV channels, newspapers, and radio stations, within a particular market or region. This consolidation of ownership can limit diversity of viewpoints and control over information dissemination. An example includes companies like Comcast owning NBCUniversal, which encompasses various media platforms.
What distracts him from his work?
He gets distracted by his phone, social media, and noisy surroundings that break his concentration.
