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Cryoprotectants work as an antifreeze to prevent formation of ice crystals and aid in the process of vitrification, a method of converting biological tissue into a strange kind of low-temperature glass that is totally free of ice crystals. Two Cryoprotectants are glycerol and DMSO (DiMethyl SulfOxide) which, when used together, enhances cryroprotection as much as double than when used alone, possibly because of a decrease in toxic effect. They work by diffusing into cells and replacing much of the body's water. Deb

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Q: How do cryoprotectants work especially DMSO?
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What Cryoprotectants used for embryonic stem cells?

Type your answer here..DMSO V used for cyropreservation..........


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How can you get a synthetic antifreeze for human bodies cells to not form crystals?

One uses a chemical (or mixture of chemicals) known as a cryoprotectant. There are a number of different cryoprotectants. The major obstacle is that many of them are toxic to human biology. Using a mixture generally reduces the toxicity since different cryoprotectants are toxic in different ways. One tries to come up with a mixture that doesn't have a toxic amount of any single cryoprotectant. Some cryprotectants include glycerol, gycols (propylene and ethylene glycols), Dimethyl sulfoxide (DMSO, a common solvent in organic chemistry) and others.


What is the difference betwen DMSO and DMSO-d6 solution?

One difference is that DMSO-d6 is going to cost more. Chemically speaking, the diffference is that the hydrogens in the -d6 variety have been replaced by deuterium.


Why is DMSO used as a control?

Many test compounds (drugs, inhibitors, etc) are not soluble in water and therefore dimethylsulfoxide (DMSO) is used as a solvent instead. The compound dissolved in DMSO is what is used to treat the cell or animal and therefore you must prove that it is the compound, not the DMSO which is causing any results seen. To ensure this, you have a control that contains only DMSO and not the test compound. This is often referred to as a "vehicle control".


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What is the role of DMSO in cell culture?

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What is the geometry of Ph3SnCl and DMSO complex?

Use the link below to begin your investigation of the geometry of Ph3SnCl and the polar aprotic solvent DMSO (dimethyl sulfoxide).


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