Hi,
For cleaning LAF you first wash your hands with alcohol. Now take a cotton and dip it in 70% alcohol and wipe in on LAF properly in each and every corner. Also rub the cotton into the holes or floor if the LAF is vertical air flow type. Clean the UV tublights and ordinary tubelights with alcohol. If there is any stain of cultural media on the floor which can not be removed by cotton then take a glass slide and scratch it with slide. After cleaning with alcohol properly now shuttdown the screen and on the UV light for atleast 20 minutes and before 5 minutes of starting your work putt of the UV light.
NOTE: Never stand in front of the LAF when UV is on it can cause mutation to you.
The laminar air flow chamber is a way to provide a sterile work area. This lowers the possibility of contamination while working with your cultures or fungi.
manometer is important because it shows the working condition of HEPA filters
laminar flow Smooth, orderly movement of a fluid, in which there is no turbulence, and any given subcurrent moves more or less in parallel with any other nearby subcurrent. Laminar flow is common in viscous fluids, especially those moving at low velocities. Its used to avoid the spreading of fumes and gases when conducting an experiment resulting in a gaseous reaction.
we can also use 100% ethanol or pure ethanol but as it has been tested that 70% is sufficient to kill all the cells and spores of microbes so use this
UV light iddivided in to three. UV-C wave length from 100nm to 280nm. UV-B 280nm to 315nm and UV-A 315nm to 400nm. The wave length of 254nm (UV-C) is giving maximum germicidal activity and it is not too irritating.
difference between laminar air flow & reverse laminar air flow
The laminar flow hood depends on the laminar air flow to sweep away pathogens from the hood. If there is obstruction, the laminar air flow pattern will be disrupted. The laminar air flow will then change to turbulent air flow.
isopropyl alcohol
Bernoulli was the first guy to study it some 250 years back - laminar fluid flow that is.
In a horizontal laminar flow hood, the laminar air flows towards the outside (i.e. towards the worker). There is hence no protection to the worker and it is not suitable for cytotoxic drug preparation. In a vertical laminar flow hood, the laminar air flows downwards from the top, providing more protection for the workers. Vertical laminar flow hoods are hence suitable for preparing cytotoxic drugs.
aminar Flows Laminar air flows can maintain a working area devoid of contaminants. Many medical and research laboratories require sterile working environments in order to carry out specialised work. Laminar Flow Cabinets can provide the solution.Why Laminar Flow Cabinets? Laminar Flow Cabinets create particle-free working environments by projecting air through a filtration system and exhausting it across a work surface in a laminar or uni-directional air stream. They provide an excellent clean air environment for a number of laboratory requirements.Uses Laminar Flow Cabinets are suitable for a variety of applications and especially where an individual clean air environment is required for smaller items, e.g. particle sensitive electronic devices. In the laboratory, Laminar Flow Cabinets are commonly used for specialised work. Laminar Flow Cabinets can be tailor made to the specific requirements of the laboratory and are also ideal for general lab work, especially in the medical, pharmaceutical, electronic and industrial sectors. How They Are Made Laminar Flow Cabinets, or laminar air flow cabinets as they are also known, are normally made of stainless steel with no gaps or joints thereby preventing the build-up of bacteria from collecting anywhere in the working zone. Laminar Flow Cabinets are also known as clean benches because the air for the working environment is thoroughly cleaned by the precision filtration process.How They Work The process of laminar air flow can be described as airflow where an entire body of air flows with steady, uniform velocity. Laminar Flow Cabinets work by the use of in-flow laminar air drawn through one or more HEPA filters, designed to create a particle-free working environment and provide product protection. Air is taken through a filtration system and then exhausted across the work surface as part of the laminar flows process. Commonly, the filtration system comprises of a pre-filter and a HEPA filter. The Laminar Flow Cabinet is enclosed on the sides and constant positive air pressure is maintained to prevent the intrusion of contaminated room air.
This question is its own answer. The flow patterns in laminar flow are laminar.
Laminar flow hoods can be found through ebay's website. Companies NuAire, Terra Universal, and Air Clean Systems all offer their own laminar flow hoods (also called laminar flow closets and tissue culture hoods) on their official websites.
Laminar Airflow describes the flow around an object such that the air stays "attached" to the surface. At higher airspeeds, the air become detached and turbulent. Thus a laminar flow chamber is one where airfoils or model airplanes are tested at low airspeeds and especially airspeeds less than Mach 1.0.
15lb pressure
The laminar air flow chamber is a way to provide a sterile work area. This lowers the possibility of contamination while working with your cultures or fungi.
Laminar Flow - album - was created in 1979.