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Why should you never look at a wet mount slide with a oil immersion lens?
Wet mounts are not attached to the slide, it is "floating". If you use an oil immersion lens, you have to put a drop of oil on the slide cover so the light can correctly focus. The oil will cause the slide cover to stick to the end of your Oil immersion lens. When you focus the field of focus will stay the same since the slip cover is stuck to the lens. When you go to change objectives, the cover will travel with your oil immersion lense.
Which objective focuses closest to the slide when it is in focus?
The objective lens that focuses closest to the slide is 100x, it has the longest lens so the highest power. Be careful not to crack the slide and make sure you use oil if it is an oil immersion lens.
When using the oil immersion lens you should raise the objective lenses for easy application of an oil droplet to the slide is this true or false?
False. When using the oil immersion lens, you should first bring the lens close to the slide without touching it, then apply a small drop of immersion oil directly onto the slide. After that, you should carefully raise the lens into the oil droplet to ensure proper immersion and optimal resolution.
Should you store the microscope with the oil immersion lens in position?
No, it is not recommended to store the microscope with the oil immersion lens in position. It can lead to the oil drying out and potentially damaging the lens or affecting the quality of future observations. It is best to clean the lens and remove excess oil after use before storing the microscope.
Should the microscope be stored with the oil immersion lens in position over the stage?
No, it is not recommended to store the microscope with the oil immersion lens in position over the stage. This could lead to accidental damage to the lens or misalignment of the microscope components. It's best to remove the oil immersion lens and store it properly to maintain its integrity and performance.
Why should you never look at a wet mount slide with a oil immersion lens?
Wet mounts are not attached to the slide, it is "floating". If you use an oil immersion lens, you have to put a drop of oil on the slide cover so the light can correctly focus. The oil will cause the slide cover to stick to the end of your Oil immersion lens. When you focus the field of focus will stay the same since the slip cover is stuck to the lens. When you go to change objectives, the cover will travel with your oil immersion lense.
Which objective focuses closest to the slide when it is in focus?
The objective lens that focuses closest to the slide is 100x, it has the longest lens so the highest power. Be careful not to crack the slide and make sure you use oil if it is an oil immersion lens.
When using the oil immersion lens you should raise the objective lenses for easy application of an oil droplet to the slide is this true or false?
False. When using the oil immersion lens, you should first bring the lens close to the slide without touching it, then apply a small drop of immersion oil directly onto the slide. After that, you should carefully raise the lens into the oil droplet to ensure proper immersion and optimal resolution.
What objective focuses closest to slide?
The oil immersion lens @100x
What is the function of the oil used with the oil immersion lens?
So that you do not scratch the lens because at 100x magnification it is very very close to the slide.
Why do you use on or slide to be examined with the oil immersion objective?
If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.
What three things have to be done before putting away a microscope?
first you turn magnification lens to lowest setting. second, you remove the slide you were looking at. third, you turn off or unplug the microscope.
Why would you use oil on a microscope slide?
Light microscopes commonly need oil for two reasons. The first is that a dab of oil will keep a specimen still on the slide holding it in place and allowing for easier manipulation. The second is that at high magnification, the large change in refractive index from the glass lens, to air, to the slide limits the resolution of the image. With oil between the lens and the sample, higher resolution is achieved.
When would you need to use the oil immersion lens?
Not only does oil immersion increase the microscopic resolution of a specimen, it is also transparent. This allows for optimal microscopy views.
Should you store the microscope with the oil immersion lens in position?
No, it is not recommended to store the microscope with the oil immersion lens in position. It can lead to the oil drying out and potentially damaging the lens or affecting the quality of future observations. It is best to clean the lens and remove excess oil after use before storing the microscope.
Which of the objectives comes closest to a specimen and is most likely to break a slide if proper precautions are not taken?
The oil immersion objective comes closest to the specimen and is most likely to break a slide if proper precautions are not taken. Oil immersion objectives require the addition of immersion oil between the objective lens and the slide to improve resolution, and without the correct amount of oil or if the slide is not handled carefully, it can lead to damage or breakage of the slide.
Why is it necessary to use emmersion oil with the 100x objective?
because at this magnification the light diffraction in air is to important to have enough light reach the lens, so oil should be in the contact between the slide and the lens because it has a much lower refraction index than air allowing more light to reach the lens
