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How do you remove oil from the slide and oil immerssion lens after use?
VivianSeniteteva
When you desire to remove oil from a slide and an immersion lens after using, for example, cedar oil, you would use Xylene to remove the oil. Natural oils can harden on the lens otherwise.
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∙ 10y ago
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Which objective focuses closest to the slide when it is in focus?
The objective lens that focuses closest to the slide is 100x, it has the longest lens so the highest power. Be careful not to crack the slide and make sure you use oil if it is an oil immersion lens.
Why should you never look at a wet mount slide with a oil immersion lens?
Wet mounts are not attached to the slide, it is "floating". If you use an oil immersion lens, you have to put a drop of oil on the slide cover so the light can correctly focus. The oil will cause the slide cover to stick to the end of your Oil immersion lens. When you focus the field of focus will stay the same since the slip cover is stuck to the lens. When you go to change objectives, the cover will travel with your oil immersion lense.
When using the oil immersion lens you should raise the objective lenses for easy application of an oil droplet to the slide is this true or false?
False
Why is it important to only use the fine adjustment knob when using the highest power objective lens?
When using high power, the lens is very close to the sample - and so you risk damaging the slide and/or lens if they touch - therefore you only want to move the platform a little bit at a time. It is very similar with an oil immersion - you don't want to damage the lens by allowing the sample to touch it.
Why is immersion oil is used in a slide?
Immersion oil forms a path between the lens and the specimen for the light to follow. The space between the lens and specimen at high magnification is tiny. If you didn't use oil, the light would bend away from the lens, and you wouldn't be able to see anything.
Which objective focuses closest to the slide when it is in focus?
The objective lens that focuses closest to the slide is 100x, it has the longest lens so the highest power. Be careful not to crack the slide and make sure you use oil if it is an oil immersion lens.
Why should you never look at a wet mount slide with a oil immersion lens?
Wet mounts are not attached to the slide, it is "floating". If you use an oil immersion lens, you have to put a drop of oil on the slide cover so the light can correctly focus. The oil will cause the slide cover to stick to the end of your Oil immersion lens. When you focus the field of focus will stay the same since the slip cover is stuck to the lens. When you go to change objectives, the cover will travel with your oil immersion lense.
What is the function of the oil used with the oil immersion lens?
So that you do not scratch the lens because at 100x magnification it is very very close to the slide.
What objective focuses closest to slide?
The oil immersion lens @100x
Why do you use on or slide to be examined with the oil immersion objective?
If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.
Why is it necessary to use emmersion oil with the 100x objective?
because at this magnification the light diffraction in air is to important to have enough light reach the lens, so oil should be in the contact between the slide and the lens because it has a much lower refraction index than air allowing more light to reach the lens
Why would you use oil on a microscope slide?
Light microscopes commonly need oil for two reasons. The first is that a dab of oil will keep a specimen still on the slide holding it in place and allowing for easier manipulation. The second is that at high magnification, the large change in refractive index from the glass lens, to air, to the slide limits the resolution of the image. With oil between the lens and the sample, higher resolution is achieved.
When using the oil immersion lens you should raise the objective lenses for easy application of an oil droplet to the slide is this true or false?
False
When would you need to use the oil immersion lens?
Not only does oil immersion increase the microscopic resolution of a specimen, it is also transparent. This allows for optimal microscopy views.
Why isn't the oil immersion lens used in hanging drop procedure?
the hanging drop procedure uses a thicker slide, which could crack if the oil immersion lens is used. My bio professor worte: hanging drop slides asre too thick to use under oil.
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