The objective lens that focuses closest to the slide is 100x, it has the longest lens so the highest power. Be careful not to crack the slide and make sure you use oil if it is an oil immersion lens.
oil immersion lens
Objective.
Scanning
100x
It's called the "working distance" and is defined as, "the distance from the front lens element of the objective to the closest surface of the coverslip when the specimen is in sharp focus."
oil immersion
When using a microscope, the initial focusing of any slide is done under either the 4x or 10x objective. Once you have your subject in focus, you may make further adjustments.
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It holds the objective lens- the lens closest to the slide. A revolving nosepiece holds several lenses, and permits them to be changed easily- changing the degree of magnification
The oil immersion lens @100x
It's called the "working distance" and is defined as, "the distance from the front lens element of the objective to the closest surface of the coverslip when the specimen is in sharp focus."
The objective lens
oil immersion
When focusing the microscope downward, you take the chance of damaging the expensive objective lens by crushing the slide trying to get it in focus. By first eyeballing the objective lens and adjusting it very close to the slide, and then moving the objective lens up for the correct focus you're safe.
The high power objective lense will most likely break the slide. You need to use coarse adjustment on the low power objective lens.
The high power objective lense will most likely break the slide. You need to use coarse adjustment on the low power objective lens.
On a microscope with the usual 3-lens turret it is usual to use the objective lens with the lowest magnification to first examine your specimen. This gives a wider overall view of the subject, and will allow you to choose the particular detail that best suits your study. You may then move on to a higher magnification, if necessary, to study finer detail. If you started with the highest magnification, your fine focus will be uncertain, and you risk the front of the objective lens coming into contact with the sample slide. This could damage your specimen, and may damage the front of the lens.
so the objective lens will not touch the slide.
In the end you will have to look into it, but at the start, when everything is far out of focus there is a real danger that as you try to find the focus you will grind the lower (objective) lens into the object, possibly destroying it, and damaging the lens at the same time.
The objective lens on a microscope that comes closest to the specimen and is most likely to break a slide if proper precautions are not taken is the high-power objective. High-power objectives are designed for high magnification, typically in the range of 40x to 100x, and they have a very short working distance. The working distance is the distance between the objective lens and the specimen. Because the high-power objective has such a short working distance, it means that it needs to be very close to the specimen being observed. If the slide or coverslip is too thick or if there's an uneven or raised area on the slide, there's a risk that the objective lens may come into contact with the slide, potentially causing damage to both the lens and the slide. This is why it's crucial to handle slides carefully and ensure they are prepared with the appropriate thickness and flatness to avoid damaging the high-power objective and obtaining clear images during microscopy.
When using a microscope, the initial focusing of any slide is done under either the 4x or 10x objective. Once you have your subject in focus, you may make further adjustments.