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The API 20E System is a miniatuized version of conventional tsts that is used for the identification of members of the family Enterobacteriaceae and other gram-negative bacteria. This system utilizes a plastic strip with 20 separated compartments. Each compartments consists of a depression, or cupule, and a small tube that contains a specific dehydrated medum. After you inoculated each compartment with an unknown bacteria, a reaction will occur within 24 hours. The results of each reaction are tabulated and then given a number based on the results. The unknown bacteria is identified by searching through a book, Analytical Profile Index (may be in other book), and searching for the number corresponding to your unknown.
The test consists of the following:
ONPG: galactosidase
ADH: Arginine DiHydolase
LDC: LysineDeCarboxylase
ODC: Ornithinine DeCarboxylase
CIT: Citrate utilization
H2S: Hydogen sulfide production
URE: Ureaase
TDA: Tryptophane DeAminase
IND: Indole Production
VP: acetoin production (Voges Proskauer)
GEL: Gelatinase
GLU: fermentaion / oxidation (Glucose)
MAN: fermentaion / oxidation (Mannitol)
INO: fermentaion / oxidation (Inositol)
SOR: fermentaion / oxidation (Sorbitol)
RHA: fermentaion / oxidation (Rhamnose)
SAC: fermentaion / oxidation (Saccharose)
MEL: fermentaion / oxidation (Melibiose)
AMY: fermentaion / oxidation (Amygdalin)
ARA: fermentaion / oxidation (Arabinose)
Supplemental Test also may be done with the API test
OXI:
NO2:
N2 GAS:
MOT:
MAC:
OF-O:
OF-F:
The reactions of each compartment are given a (+) or (-). Each compartment has a corresponding points. If a reaction for a certain compartment, like GLU, is possible then the points are counted.
Every 3 compartment (or test) is given a number. The points of every 3 set is 1, 2, and 4 (in order)... Do not get mixed up with points and number. Each test has a corresponding points and Every 3 tests is given a number based on the results...
For example:
ONPG (1), ADH (2), and LDC (4)
If ONPG and LDC are positive and ADH reaction is negative, then the number given to this set is 5 (1pt from ONPG and 4pt from LDC; No point is given to ADH because the result is negative)
THERE SHOULD BE 9 NUMBERS
[27 tests/compartments divided into 3 groups each is 9]
A test result for a specific bacteria of Eshcerichia coli (E. coli) is
+ - + + - - - - + - - + + - + + - + - + - + - + + + +
5 1 4 4 5 5 2 5 7
The number is 514455257
An important thing to remember is that they have a lot of bacteria. Some bacterias have different strains, so there may be more than 1 number that identifies to be E. coli. It's just they are different strains of E. coli
Information taken from Benson's MICROBIOLOGY APPLICATIONS Tenth edition by Alfred E. Brown
Posted by 13K
What else can I help you with?
Why is it important to check the Gram stained film of the organism before setting up the API 10s strip?
It is important to check the Gram stained film of the organism before setting up the API 10s strip because the API system relies on accurate identification of the organism for determining biochemical reactions. Gram staining helps in determining the type of bacteria (Gram-positive or Gram-negative), which can guide in interpreting API test results accurately. Incorrect identification based on Gram staining can lead to misinterpretation of API results and incorrect identification of the organism.
What does abbreviaton API stand for in microbiology?
In microbiology, the abbreviation API stands for Analytical Profile Index, which is a method used to identify and classify bacteria based on their biochemical characteristics. This system uses a series of biochemical tests to compare the growth patterns of an unknown bacteria with a database of known bacteria for identification.
What does the test for UN in blood work mean?
A test for UN in blood work typically refers to measuring blood urea nitrogen, which is a waste product filtered by the kidneys. Elevated levels can indicate kidney dysfunction, dehydration, or certain medical conditions affecting the kidneys. Low levels can be associated with liver disease or a low-protein diet.
What is the term of a blood test called?
A blood test is also known as a blood panel or blood work. It involves analyzing a sample of blood to evaluate various aspects of health such as blood cell count, cholesterol levels, and presence of infections or diseases.
Why is heat applied in Benedict's test and not alcohol emulsion test?
In Benedict's test, heat is applied to help facilitate the redox reaction between reducing sugars and the copper (II) ions in the Benedict's reagent, leading to the formation of a colored precipitate. In the alcohol emulsion test, the presence of lipids is indicated by the formation of a white turbidity or opaque layer when lipids are mixed with alcohol and water, and heat is not necessary for this observation.
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What is difference between API 6D and API 598?
The pressure test for API 598 is slightly less pressure and less duration than API 6D. API 6D is specifically for pipeline valves.
What is API 675 hydrostatic test?
API 675 says that the metering pump has to be tested at 1.5 times the MAWP (Maximum Allowable Working pressure) but not less than 20psig (API 675 4.3.2.1)
What is the bar-over test of a compressor?
see API 618 description
Will API Service SM motor oil work in 2004 Honda Shadow?
It depends. If the manual says it will work, then yes. Otherwise, API Service SM has friction modifiers that are not compatible with motorcycle's wet clutches.
Why is an oxidase test performed on a culture before using API 20E and Enterotube II to identify the culture?
The oxidase test is performed to determine if the culture contains cytochrome c oxidase enzyme, which helps in distinguishing between oxidase-positive and oxidase-negative organisms. This information is important for selecting the appropriate biochemical tests in the API 20E and Enterotube II identification systems, as these tests are designed to work best with specific types of bacteria based on their oxidative characteristics.
Does API 6D meet or exceed API 598?
API 6D exceeds API 598 in both pressure and duration.
