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How she was able to add the HInd III restriction enzyme site in this position based on the result of her gel?
Wiki User
∙ 13y ago
She was able to do so because the band pattern portrayed on the agarose gel shows exactly where the sequence AAGCTT was located on the DNA. Because of this, she was able to place the Hind III restriction enzyme right at that sequence since it was given that the enzyme recognizes that specific sequence.
Wiki User
∙ 13y ago
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Why the first restriction endonuclease is known as Hind2 and not Hind1?
Restriction enzymes are named based on the organism in which they were discovered. For example, the enzyme Hind III was isolated from Haemophilus influenzae, strain Rd. The first three letters of the name are italicized because they abbreviate the genus and species names of the organism. The fourth letter typically comes from the bacterial strain designation. The Roman numerals are used to identify specific enzymes from bacteria that contain multiple restriction enzymes. Typically, the Roman numeral indicates the order in which restriction enzymes were discovered in a particular strain.There are three classes of restriction enzymes, labeled types I, II, and III. Type I restriction systems consist of a single enzyme that performs both modification (methylation) and restriction activities. These enzymes recognize specific DNA sequences, but cleave the DNA strand randomly, at least 1,000 base pairs(bp) away from the recognition site. Type III restriction systems have separateenzymes for restriction and methylation, but these enzymes share a common subunit. These enzymes recognize specific DNA sequences, but cleave DNA at random sequences approximately twenty-five bp from the recognition sequence. Neither type I nor type III restriction systems have found much application in recombinant DNA techniques.Type II restriction enzymes, in contrast, are heavily used in recombinant DNA techniques. Type II enzymes consist of single, separate proteins for restriction and modification. One enzyme recognizes and cuts DNA, the other enzyme recognizes and methylates the DNA. Type II restriction enzymes cleave the DNA sequence at the same site at which they recognize it. The only exception are type IIs (shifted) restriction enzymes, which cleaveDNA on one side of the recognition sequence, within twenty nucleotides of the recognition site. Type II restriction enzymesdiscovered to date collectively recognize over 200 different DNA sequences.
Based on restriction maps of plasmid determine the number of DNA fragments and sizes of the fragments?
Plasmids are circular pieces of DNA, so the number of fragments equals the number of cuts from the restriction enzymes. You can easily see this if you start with one restriction enzyme that cuts the plasmid in only one place. Cutting the circle in one place yields you only one fragment. If the restriction cuts in two places, you end up with two fragments; with three places, three fragments, etc. With linear chromosomes, the situation is different. Cutting a linear chromosome in one place yields two fragments, cutting in two places yields three fragments, etc. So the number of fragments is always one more than the number of cuts. A restriction map of a plasmid will show all of the cuts the restriction enzymes made. Each cut is labeled with the enzyme that made it. One can count the spaces between cuts to determine the number of fragments that are produced. Restriction maps usually (but not always) also show the size of each fragment.
What enzyme transcribes DNA?
The enzyme that transcribes the DNA into RNA is called RNA polymerase.
The initial rate of an enzyme catalysed reaction depend on?
Based on Michaelis-Menten enzyme kinetics, the initial rate of reaction, vi, is dependent on maximum rate Vmax, substrate concentration [S], and the enzyme's Michaelis constant Km, which represents the the tendency of the substrate/enzyme complex to dissociate. The dependence on enzyme concentration is factored into the maximum rate. The equation to describe this is: vi = Vmax([S]/(Km+[S])) Follow the link below for details.
The use of RFLPs are genetic fingerprinting is based on?
Originally, yes. RFLP stands for Restriction Fragment Length Polymorphism. An enzyme which cuts DNA is added to a sample of DNA purified from blood or whatever tissue is available for the test. The enzyme will cut DNA only at particular sites, yielding many segments of cut DNA. The pattern of cuts can be seen by taking this partially digested DNA and running it through a gel to separate the smaller fragments from the larger fragments. This produces a set of distinctive bands that are essentially unique to every individual on the planet, although members of the same family may have similar patterns.For more details, refer to http:/en.wikipedia.org/wiki/DNA_profiling .
Based on the results of what conclusions can be drawn about the action of the enzyme as the temperature increase?
enzyme A becomes less effective earlier than enzyme B enzyme b stays effective at higher temperatures than enzyme a
Why the first restriction endonuclease is known as Hind2 and not Hind1?
Restriction enzymes are named based on the organism in which they were discovered. For example, the enzyme Hind III was isolated from Haemophilus influenzae, strain Rd. The first three letters of the name are italicized because they abbreviate the genus and species names of the organism. The fourth letter typically comes from the bacterial strain designation. The Roman numerals are used to identify specific enzymes from bacteria that contain multiple restriction enzymes. Typically, the Roman numeral indicates the order in which restriction enzymes were discovered in a particular strain.There are three classes of restriction enzymes, labeled types I, II, and III. Type I restriction systems consist of a single enzyme that performs both modification (methylation) and restriction activities. These enzymes recognize specific DNA sequences, but cleave the DNA strand randomly, at least 1,000 base pairs(bp) away from the recognition site. Type III restriction systems have separateenzymes for restriction and methylation, but these enzymes share a common subunit. These enzymes recognize specific DNA sequences, but cleave DNA at random sequences approximately twenty-five bp from the recognition sequence. Neither type I nor type III restriction systems have found much application in recombinant DNA techniques.Type II restriction enzymes, in contrast, are heavily used in recombinant DNA techniques. Type II enzymes consist of single, separate proteins for restriction and modification. One enzyme recognizes and cuts DNA, the other enzyme recognizes and methylates the DNA. Type II restriction enzymes cleave the DNA sequence at the same site at which they recognize it. The only exception are type IIs (shifted) restriction enzymes, which cleaveDNA on one side of the recognition sequence, within twenty nucleotides of the recognition site. Type II restriction enzymesdiscovered to date collectively recognize over 200 different DNA sequences.
What is the enzyme-based treatment used by Gonzalez?
Gonzalez uses his enzyme-based treatment on patients with pancreatic cancer, as well as a wide variety of other cancers.
If an enzyme is a protein how might you change the specificity of such an enzyme?
What an enzyme does is based on its shape, therefore you would have to change it on a molecular level in order to alter its job.
Based on restriction maps of plasmid determine the number of DNA fragments and sizes of the fragments?
Plasmids are circular pieces of DNA, so the number of fragments equals the number of cuts from the restriction enzymes. You can easily see this if you start with one restriction enzyme that cuts the plasmid in only one place. Cutting the circle in one place yields you only one fragment. If the restriction cuts in two places, you end up with two fragments; with three places, three fragments, etc. With linear chromosomes, the situation is different. Cutting a linear chromosome in one place yields two fragments, cutting in two places yields three fragments, etc. So the number of fragments is always one more than the number of cuts. A restriction map of a plasmid will show all of the cuts the restriction enzymes made. Each cut is labeled with the enzyme that made it. One can count the spaces between cuts to determine the number of fragments that are produced. Restriction maps usually (but not always) also show the size of each fragment.
The use of RFLPs for genetic fingerprinting is based on?
The use of RFLPs in generic fingerprinting is based on the ability of restriction enzymes to dissect DNA into small fragments. There are many kinds of restriction enzymes made to cut various DNA sequences.
Is the value of a digit based on its position in a number?
no the value of the number is not based on the position it is based on what the place value is
The protein-based digestive enzyme endopeptidase is produced?
during interphase
What enzyme build mrna molecule based on their on code in DNA?
RNA polymerase
What is a conclusion based on?
A conclusion is based on a result
The following steps must be performed to make a bacterium produce human protein X?
1 is translation. 2 is restriction enzyme. 3 is prokaryotic transcription. 4 is DNA ligase. 5 is transformation. 6 is eukaryotic transcription. 7 is reverse transcription. So the order of the steps based off of the numbers are 6,7,2,4,5,3,1.
Can a corporation own a condominium and lease it under a family lease restriction?
The answer you want is based on the legal documents involved, which may include the family lease restriction language and the condominium's rental language contained in the CC&Rs for the association.
