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Electrophoresis is the motion of particles relative to some fluid influenced by an electric field. The voltage used will affect this electric field, and in turn affect the movement of particles.

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Can gel electrophoresis be used to determine the purity of an enzyme?

Gel electrophoresis can be used to assess the purity of an enzyme by separating different proteins based on size. If the enzyme appears as a single band on the gel, it suggests high purity. Contaminants or impurities would result in additional bands on the gel.


What types of variations would be most detectable by gel electrophoresis if the differences were between two recognition sites for a restriction enzyme?

The most detectable variations would be insertions or deletions that alter the size of the DNA fragment between the two recognition sites for the restriction enzyme. These modifications would result in different migration distances during gel electrophoresis, allowing for easy differentiation of the samples based on their fragment sizes.


How can a mutation that alters a recognition site be detected in gel electrophoresis?

The point mutation has to result in either the removal of a restriction site of the restriction enzymes or the formation of a new one, such that the bands of mutated DNA that form after performing gel electrophoresis are different from the normal one. So a difference in banding patterns would mean that there is a point mutation.


If you have a restriction enzyme that cuts a piece of DNA at two recognition sites how many DNA fragments would you see on a gel?

Three.To see why, cut a piece of string in two places! Of course, strictly you would not be able to see only three fragments. You would amplify the DNA before carrying out electrophoresis. That way, you would get perhaps 200 million copies of each fragment, and they would show up. Also, you would only be able to distinguish the fragments if they were different lengths. Electrophoresis separates pieces of DNA by length.


How can electrophoresis gels be used to implicate any suspect in a crime?

Electrophoresis per se cannot be used to implicate a suspect. Rather, electrophoresis is merely one of many analytical techniques which may be applied to a specific question, being posed by a an investigator. The value of electrophoresis is nil outside the data quality objectives (DQOs) presented by the investigating team, and the QA/QC program of the analyzing laboratory. Without QA/QC, in the lab, and without appropriate DQOs, electrophoresis is no more capable of implicating a suspect than a ouija board. Laboratories cannot perform in the real world as seen on TV's CSI, and real CSIs would go to jail if they pulled some of the stunts seen on the TV series.

Related Questions

What is reverse magnetic polarity?

lets say the earth was to have a magnetic polarity reversal the north pole would become the south pole, and the south pole would become the north pole. i hope that helps.


Would husky pay for tubal reversal in ct?

will husky pay for a reversal


Which type of evidence would be the most appropriate for electrophoresis analysis?

Blood


What would altered conditions in electrophoresis do?

who was the first astronomer to see mountains on the moom


Discuss how each what factors would affect the results of electrophoresis?

A person will not be able to discuss how the following factors would affect the results of electrophoresis unless they know what the following factors are. This information needs to be included for a person to know the correct answer.


What is a synonym for the word reversal?

Some synonyms one could use for the word reversal would be annulment, ending, void or withdrawal. One could also use the term turn back instead of reversal.


How would this history of reversal show itself on the ocean floor?

it tugs on the iron pieces in the sand causing ripples.


What would happen if Earth's magnetism get reversed?

There would be a temporary weakening of the magnetic field during the reversal. After the reversal the only noticeable difference would be that compasses now point south instead of north. Navigation systems that use the magnetic field would need to be recalibrated.


Is 12 volts peak to peak the same as 12 volts ac?

No. 12 volts peak to peak would be 6 in the positive polarity and 6 negative polarity. Simply saying 12 volts AC would be 12 volts in each polarity or 24 volts peak to peak


How does gel electrophoresis is used to make a DNA fingerprint?

Gel electrophoresis separates DNA fragment on the basis of their size. In DNA fingerprinting or DNA typing given sample is cut up with restriction enzymes and run through electrophoresis and results are analyzed to check for DNA polymorphism between the given sample and a sample form suspect. In nutshell gel electrophoresis is boon for the people in forensics.


Can electric lines of force cross each other explain?

No, they must always point from one polarity of charge to the opposite polarity. This would not be true if they crossed.


What kind of pattern of bands on an electrophoresis gel would show from the DNA samples taken the Dolly?

They would be the same since Dolly is clone.