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Can gel electrophoresis be used to determine the purity of an enzyme?
Wiki User
∙ 16y ago
Yes it can be done if you use Poly-Acrylamide Gel Electrophoresis.
Wiki User
∙ 16y ago
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What is used to sort DNA into different lenghts?
Gel Electrophoresis
What type of cofactor is most likely to be used by an enzyme that operates outside of a cell?
It is not possible to determine whether an enzyme requires a cofactor from these data.
What are the common stains that is used after DNA electrophoresis?
Ethidium bromide and coomassie blue are some stains that can be used in DNA electrophoresis.
Is electrophoresis part of biotechnology?
Electrophoresis is used to separate molecules based on size and charge. Since biotechnology depends on knowing what you are working with, electrophoresis of proteins, DNA and RNA is a tool used by biotechnologists.
A technique that can be used to compare the DNA of two or more plants is?
Gel electrophoresis
What are the application of paper electrophoresis?
Paper electrophoresis is used to analyze scientific experiments. One use in scientific experiments for paper electrophoresis is to determine the presence of HIV from blood samples.
What is used to sort DNA into different lenghts?
Gel Electrophoresis
What type of cofactor is most likely to be used by an enzyme that operates outside of a cell?
It is not possible to determine whether an enzyme requires a cofactor from these data.
Why do you use electrophoresis?
Estimation of the size of DNA molecules following restriction enzyme digestion, e.g. in restriction mapping of cloned DNA.Analysis of PCR products, e.g. in molecular genetic diagnosis or genetic fingerprintingSeparation of restricted genomic DNA prior to Southern transfer, or of RNA prior to Northern transfer.Gel electrophoresis is used in forensics, molecular biology, genetics, microbiology and biochemistry. The results can be analyzed quantitatively by visualizing the gel with UV light and a gel imaging device. The image is recorded with a computer operated camera, and the intensity of the band or spot of interest is measured and compared against standard or markers loaded on the same gel. The measurement and analysis are mostly done with specialized software.Depending on the type of analysis being performed, other techniques are often implemented in conjunction with the results of gel electrophoresis, providing a wide range of field-specific applications.
What are the common stains that is used after DNA electrophoresis?
Ethidium bromide and coomassie blue are some stains that can be used in DNA electrophoresis.
What is a restiction enzyme?
A restriction enzyme is used to cut DNA (either double or single stranded), cuts can either be "sticky" ( in which cut pieces overlap one another), or "blunt" (in which cut pieces do not overlap). A common rectriction enzyme is EcoR1.
Is electrophoresis part of biotechnology?
Electrophoresis is used to separate molecules based on size and charge. Since biotechnology depends on knowing what you are working with, electrophoresis of proteins, DNA and RNA is a tool used by biotechnologists.
A technique that can be used to compare the DNA of two or more plants is?
Gel electrophoresis
What is the purpose of a purity test?
Purity tests are used to determine the extent to which a particular substance is what is claimed. A classic purity test for gold is nitric acid; if the item being examined reacts to the acid it is not gold, or at least not pure gold.
Difference between vertical and horizontal gel electrophoresis?
Horizantal gel electrophoresis is generally used for RNA/DNA based studies, while vertical gel electrophoresis is used for protein based studies.
What instrument measures the purity of Argon gas?
An argon gas analyzer measures the purity of Argon gas. The analyzer can be used to determine the actual content of Argon gas in a specific sample.
What is a DNA size standard and why is it used in gel electrophoresis?
You may be referring to the DNA ladder used in gel electrophoresis. The ladder is a collection of DNA fragments of known size (e.g. 100, 500, 1000, 2000, 5000, 10000 base pairs) so that if it is loaded beside the samples, it can offer a 'ruler' that can be used to determine the size of the fragments in the samples.
