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- Estimation of the size of DNA molecules following restriction enzyme digestion, e.g. in restriction mapping of cloned DNA.
- Analysis of PCR products, e.g. in molecular genetic diagnosis or genetic fingerprinting
- Separation of restricted genomic DNA prior to Southern transfer, or of RNA prior to Northern transfer.
Gel electrophoresis is used in forensics, molecular biology, genetics, microbiology and biochemistry. The results can be analyzed quantitatively by visualizing the gel with UV light and a gel imaging device. The image is recorded with a computer operated camera, and the intensity of the band or spot of interest is measured and compared against standard or markers loaded on the same gel. The measurement and analysis are mostly done with specialized software.
Depending on the type of analysis being performed, other techniques are often implemented in conjunction with the results of gel electrophoresis, providing a wide range of field-specific applications.
What else can I help you with?
Some viva voce questions for electrophoresis?
1. WHAT IS ELECTROPHORESIS AND WHAT ARE THE IMPORTANTAPPLICATIONS OF ELECTROPHORESIS?Ans. Movement of charged particle in the electric field either towards cathode or anode whensubjected to an electric current is called electrophoresis.The following factors influence the movement of particles during the electrophoresis.(a) Electric current.(b) Net charge of the particle.(c) Size and shape of the particle.(d) Type of supporting media.(e) Buffer solution.Important Applications of ElectrophoresisThe technique of electrophoresis is used to separate and identify the(i) Serum proteins(ii) Serum lipoproteins(iii) Blood hemoglobins2. WHAT ARE THE DIFFERENT TYPES OF ELECTROPHORESIS?Ans. (a) Moving boundary electrophoresis: This technique was first introduced by TISELIUS in 1937(b) Zone electrophoresis: In this type of electrophoresis different types of supporting mediaare used. These are;(a) Paper electrophoresis(i) Whatman filter paper(ii) Cellulose acetate(b) Gel electrophoresis(i) Agarose.(ii) Polyacrylamide gel (used for the separation of isoenzymes).(iii) SDS-PAGE.(iv) Iso-electric focussing (proteins seperated in a medium possessing a stable pH gradient).(v) Immuno electrophoresis (for the separation of immunoglobulins).
What color tube do you use to draw Protein electrophoresis?
Typically, a lavender or purple-top tube is used to collect a blood sample for protein electrophoresis testing. These tubes contain EDTA as an anticoagulant to prevent clotting and preserve the blood sample for analysis.
How to measure migration distance in gel electrophoresis?
To measure migration distance in gel electrophoresis, you can use a ruler or a specialized software to measure the distance the DNA or protein bands have traveled from the starting point in the gel. This distance is typically measured in millimeters or centimeters.
Does gel electrophoresis separate molecules based on their charge?
Yes, gel electrophoresis separates molecules based on their size and charge.
What color tube is drawn for a hgb electrophoresis?
A lavender-top (EDTA) tube is typically used for drawing blood samples for hemoglobin electrophoresis testing.
What are the application of paper electrophoresis?
Paper electrophoresis is used to analyze scientific experiments. One use in scientific experiments for paper electrophoresis is to determine the presence of HIV from blood samples.
Which technique was use to produce these bands?
Gel electrophoresis
Scientists use gel electrophoresis to cut dna at a specific nucleotide True or false?
It is true that Scientists use gel electrophoresis to cut DNA molecules at a specific sequence of nucleotides.
Is tgge and dgge same thing?
Temperature Gradient Gel Electrophoresis (TGGE) is a refinement of Denaturing Gradient Gel Electrophoresis (DGGE). Both use the same principles.
For what purpose might one use capillary electrophoresis?
Capillary electrophoresis is a technique used in laboratories to separate molecules based on their charge in order to study and analyze them. Capillary electrophoresis uses an electric charge to force the movement of molecules since each molecule will go a varying distance based on the weight of the molecule and their charge. Some areas of study that use capillary electrophoresis include DNA sequencing and pharmaceutical analysis.
What has the author A J Houtsmuller written?
A. J. Houtsmuller has written: 'Agarose-gel-electrophoresis of lipoproteins' -- subject(s): Blood protein electrophoresis, Electrophoresis, Gel electrophoresis, Lipoproteins
When was Electrophoresis - journal - created?
Electrophoresis - journal - was created in 1980.
What use is bacteria to copy DNA?
recombinant DNA technolog- apex
What is a common name for gel electrophoresis?
Agarose gel electrophoresis.
What has the author B J Haywood written?
B. J. Haywood has written: 'Electrophoresis - technical applications' -- subject(s): Abstracts, Bibliography, Electrophoresis 'Electrophoresis-technical application' -- subject(s): Bibliography, Electrophoresis
First reports of the use of sucrose for gel electrophoresis?
The first reports of sucrose being used for gel electrophoresis date back to the 1960s. Sucrose was initially used as the gel matrix for studying certain biological molecules, showcasing its ability to separate molecules by size. This paved the way for the development of more sophisticated gel electrophoresis techniques using different types of matrices.
what is the gel in Gel Electrophoresis?
The gel in gel electrophoresis is typically made of agarose or polyacrylamide. It acts as a matrix to separate DNA, RNA, or proteins based on size and charge as an electric current passes through it. Agarose gels are commonly used for DNA analysis, while polyacrylamide gels are often used for higher resolution protein separation.
