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- Estimation of the size of DNA molecules following restriction enzyme digestion, e.g. in restriction mapping of cloned DNA.
- Analysis of PCR products, e.g. in molecular genetic diagnosis or genetic fingerprinting
- Separation of restricted genomic DNA prior to Southern transfer, or of RNA prior to Northern transfer.
Gel electrophoresis is used in forensics, molecular biology, genetics, microbiology and biochemistry. The results can be analyzed quantitatively by visualizing the gel with UV light and a gel imaging device. The image is recorded with a computer operated camera, and the intensity of the band or spot of interest is measured and compared against standard or markers loaded on the same gel. The measurement and analysis are mostly done with specialized software.
Depending on the type of analysis being performed, other techniques are often implemented in conjunction with the results of gel electrophoresis, providing a wide range of field-specific applications.
Wiki User
∙ 10y ago
Wiki User
∙ 15y agoElectrophoresis can be used to determine the mass of an object, for example a protein or DNA. A short strand of DNA will move faster in an acrylamide gell during electrophoresis, whereas a long strand of DNA will travel slower.
DNA markers (or whatever marker is relevant) of known mass are used to estimate the size of the objects traveling once electrophoresis has completed.
Electrophoresis is probably the most used tool for a molecular biologist or biochemist. There are other uses for electrophoresis that I haven't mentioned.
Wiki User
∙ 13y agoGel electrophoresis is an invaluable analytical method used to determine the molecular weight of DNA, RNA or protein samples
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What is the role of EtBr in electrophoresis?
Ethidium bromide interchalates with DNA. It doesn't affect electrophoresis, but it help visualise the DNA bands after electrophoresis. The EtBr that is bound to the DNA will fluoresce under ultraviolet light.
Some viva voce questions for electrophoresis?
1. WHAT IS ELECTROPHORESIS AND WHAT ARE THE IMPORTANTAPPLICATIONS OF ELECTROPHORESIS?Ans. Movement of charged particle in the electric field either towards cathode or anode whensubjected to an electric current is called electrophoresis.The following factors influence the movement of particles during the electrophoresis.(a) Electric current.(b) Net charge of the particle.(c) Size and shape of the particle.(d) Type of supporting media.(e) Buffer solution.Important Applications of ElectrophoresisThe technique of electrophoresis is used to separate and identify the(i) Serum proteins(ii) Serum lipoproteins(iii) Blood hemoglobins2. WHAT ARE THE DIFFERENT TYPES OF ELECTROPHORESIS?Ans. (a) Moving boundary electrophoresis: This technique was first introduced by TISELIUS in 1937(b) Zone electrophoresis: In this type of electrophoresis different types of supporting mediaare used. These are;(a) Paper electrophoresis(i) Whatman filter paper(ii) Cellulose acetate(b) Gel electrophoresis(i) Agarose.(ii) Polyacrylamide gel (used for the separation of isoenzymes).(iii) SDS-PAGE.(iv) Iso-electric focussing (proteins seperated in a medium possessing a stable pH gradient).(v) Immuno electrophoresis (for the separation of immunoglobulins).
What is the definition for paper electrophoresis?
it is a separation technique
What process uses electricity to seprate organic compounds?
electrophoresis=)
What process uses electricity to seperate organic compounds?
electrophoresis
what is the gel in Gel Electrophoresis?
For larger molecules like proteins we use polyacrylamide gel electrophoresis (PAGE). For smaller pieces like DNA we use agarose gel electrophoresis
Can gel electrophoresis be used to determine the purity of an enzyme?
Yes it can be done if you use Poly-Acrylamide Gel Electrophoresis.
What are the application of paper electrophoresis?
Paper electrophoresis is used to analyze scientific experiments. One use in scientific experiments for paper electrophoresis is to determine the presence of HIV from blood samples.
Which technique was use to produce these bands?
Gel electrophoresis
First reports of the use of sucrose for gel electrophoresis?
Gel electrophoresis is the analysis and separation method of DNA, RNA and proteins. The first reported use of this method was in 1930s.
What is protein marker and what is its use in electrophoresis?
it is a marker for specific protein identification.in electrophoresis it is used to identify particular protein based on the molecular weight.
Scientists use gel electrophoresis to cut dna at a specific nucleotide True or false?
It is true that Scientists use gel electrophoresis to cut DNA molecules at a specific sequence of nucleotides.
What is used to sort DNA into different lenghts?
Gel Electrophoresis
Is tgge and dgge same thing?
Temperature Gradient Gel Electrophoresis (TGGE) is a refinement of Denaturing Gradient Gel Electrophoresis (DGGE). Both use the same principles.
For what purpose might one use capillary electrophoresis?
Capillary electrophoresis is a technique used in laboratories to separate molecules based on their charge in order to study and analyze them. Capillary electrophoresis uses an electric charge to force the movement of molecules since each molecule will go a varying distance based on the weight of the molecule and their charge. Some areas of study that use capillary electrophoresis include DNA sequencing and pharmaceutical analysis.
The tool scientists use to load DNA into a gel for electrophoresis is called a?
Micropippette.
What has the author A J Houtsmuller written?
A. J. Houtsmuller has written: 'Agarose-gel-electrophoresis of lipoproteins' -- subject(s): Blood protein electrophoresis, Electrophoresis, Gel electrophoresis, Lipoproteins
