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In the binding of RNA polymerase to a gene requires the aid of a transcription factor?
Wiki User
∙ 14y ago
Like prokaryotes, eukaryotes must regulate gene expression. This is accomplished primarily by controlling when RNA polymerase binds to the beginning of a gene. This binding cannot take place in eukaryotes without the aid of transcription factor.
Wiki User
∙ 14y ago
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What is the function of sigma factor in transcription?
when RNA Polymerase meet the correct promoter(TATA box), it will bind at that region and then sigma factor will also bind to the RNA Polymerase.once ATP give energy, sigma factor will dissoiates from RNA Polymerase and the enzyme start to unwind the double helix
What is the role of the transactivation domain?
Transactivation domains are regions on a transcription factor (protein) which helps to activate transcription by contacting transcription factor. This is believed to be done to recruit the general transcription factors onto the gene promoter region.
Transcription?
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What is the attachment site for RNA polymerase?
according to information from http://www.rothamsted.ac.uk/notebook/courses/guide/trans.htm " if the RNA polymerase attaches to a special sequence called a promoter, an additional small protein, the factor sigma, will also attach to the polymerase and lock it on the DNA. The factor 'sigma' will only attach itself to the complex DNA / RNA polymerase when the RNA polymerase is attached to a promoter. Another hypothesis is that the factor sigma attaches to RNApol anyway and the enzyme is then able to slide along the DNA until it finds a promoter. It prevents detaching and speeds up promoter location, and decreases the affinity of RNApol for general regions of DNA. " Therefore, the answer seems to be, RNA attaches to DNA through a small protein called the factor sigma once the RNA polymerase attaches itself to a chain sequence called a "promoter". according to information from http://www.rothamsted.ac.uk/notebook/courses/guide/trans.htm " if the RNA polymerase attaches to a special sequence called a promoter, an additional small protein, the factor sigma, will also attach to the polymerase and lock it on the DNA. The factor 'sigma' will only attach itself to the complex DNA / RNA polymerase when the RNA polymerase is attached to a promoter. Another hypothesis is that the factor sigma attaches to RNApol anyway and the enzyme is then able to slide along the DNA until it finds a promoter. It prevents detaching and speeds up promoter location, and decreases the affinity of RNApol for general regions of DNA. " Therefore, the answer seems to be, RNA attaches to DNA through a small protein called the factor sigma once the RNA polymerase attaches itself to a chain sequence called a "promoter". role of sigmaActually RNA Polymerase can bind to DNA anywhere in the entire genome but sigma factor attaches to polymerase only when it is at promotor. sigma factor dissociates when polymerase crosses promotor. sigma factor stablises the pre initiatiation complex. Actually there are many promoter and many genes but which gene to be transcribed is decided by sigma factor.
What are the steps involved in the process of transcription?
DNA unwinds/"unzips" as the Hydrogen Bonds Break.The free nucleotides of the RNA, pair with complementary DNA bases.RNA sugar-phosphate backbone forms. (by RNA Polymerase.)Hydrogen bonds of the untwisted RNA+DNA "ladder" break, freeing the new RNA
When a negative transcription factor is added to the regulatory region of DNA, what happens?
DNA
What are the proteins that bind to DNA and turn on operons by making it easier for RNA polymerase to bind to a promoter called?
transcription factor
What is the function of sigma factor in transcription?
when RNA Polymerase meet the correct promoter(TATA box), it will bind at that region and then sigma factor will also bind to the RNA Polymerase.once ATP give energy, sigma factor will dissoiates from RNA Polymerase and the enzyme start to unwind the double helix
What has the author Taeeba Ijaz written?
Taeeba Ijaz has written: 'Anthraquinone-peptide conjugates as inhibitors of DNA transcription factor binding'
What has the author Xeni Provatopoulu written?
Xeni Provatopoulu has written: 'Structural and dynamic analysis of the DNA binding domain of the transcription factor AreA'
What has the author Lihor Ofira Abraham written?
Lihor Ofira Abraham has written: 'Metal binding and activity of the Helicobacter pylori transcription factor NikR'
What has the author Lan-Chau Thi Kha written?
Lan-Chau Thi Kha has written: 'Characterization of pRb and TFIIB binding by hSSU72 transcription factor'
What has the author Gerald T Quon written?
Gerald T. Quon has written: 'The landscape of false-positive transcription factor binding site predictions in yeast'
What do the promoter region do in the DNA translation?
The promoter region has no role in translation. It is the site where RNA Polymerase binds to initiate transcription. Basically, talking about bacteria, a protein called sigma70 binds to the promoter and recruits the RNA Polymerase enzyme. After the RNA Pol is recruited, the sigma70 factor is released and RNA Polymerase synthesizes the complementary mRNA from the DNA that is being transcribed.
How does transcription end?
Transcription ends once the a termination sequence is reached. The sequence depends on which polymerase is being used and if the organism is a eukaryote or prokaryote. In Eukaryotes when RNA polymerase 1 is used the termination sequence is an 18 nucleotide long sequence. For RNA polymerase 3 the termination sequence is a short sequence of Uricils but the hairpin loop is not formed as it is in prokaryotes. For RNA polymerase 2 transcription is terminated and cleavage takes place 10-35 nucleotides downstream of the AAUAA sequence. For prokaryotes, termination can occur 2 ways. Termination can occur once the termination sequence is reached or using a protein called Rho factor. For termination without Rho factor, the termination sequence is short and rich in Guanines and Cytosines followed by many Uricils in a row. A-U bonds are weaker than G-C bonds, the string of U-A bonds are easily broken and release the RNA strand Using a Rho factor, once the Terminal sequence is reached, the Rho factor binds to a sequence 50-90 bases long and unwinds the DNA from the RNA , moving towards the 3' end, releasing the RNA
What is the role of the transactivation domain?
Transactivation domains are regions on a transcription factor (protein) which helps to activate transcription by contacting transcription factor. This is believed to be done to recruit the general transcription factors onto the gene promoter region.
What is a transcription factor?
regulatory proteins
