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In prokaryotes where does DNA replication occur in the cell?
In prokaryotes, DNA replication occurs in the cytoplasm. The replication process begins at the origin of replication on the DNA molecule and proceeds bidirectionally. Multiple replication fork structures are formed to speed up the replication process.
Why does replication in prokaryotes differs from replication in eukaryotes?
Replication in prokaryotes differs from replication in eukaryotes for prokaryotic chromosomes have a single origin of replication, whereas eukaryotic chromosomes have many. Eukaryotes and prokaryotes for replication double stranded DNA, four kinds of dNTPS, primers, and origins.
How does DNA replication differ in prokaryotes and eukaryotes?
1. In eukaryotic cells replication forks make several start sites along the DNA strand which forms replication "bubbles" which get larger the more DNA is copied, and stop when DNA replication is complete. In prokaryotic cell's DNA is formed in a loop, two replication forks start along one part of the loop (origin replication) and the replication forks copy DNA in opposite directions until they meet at the other side of the loop, making an exact copy of DNA.
Do replication forks speed up replication?
Yes, replication forks do speed up the replication process by allowing DNA synthesis to occur simultaneously in both directions around the circular DNA molecule in prokaryotes or at the two replication forks in eukaryotes. This helps to expedite the replication process and minimize the time needed for DNA replication.
What are enzymes that have a proofreading role in DNA replication?
DNA polymerases, such as DNA polymerase III in prokaryotes and DNA polymerase delta in eukaryotes, have proofreading activities during DNA replication. These enzymes possess exonuclease activity, allowing them to detect and correct errors in newly synthesized DNA strands by removing misincorporated nucleotides. This proofreading function helps maintain the fidelity of DNA replication.
In prokaryotes where does DNA replication occur in the cell?
In prokaryotes, DNA replication occurs in the cytoplasm. The replication process begins at the origin of replication on the DNA molecule and proceeds bidirectionally. Multiple replication fork structures are formed to speed up the replication process.
How many replication forks are produced when DNA denatures at an origin?
Two replication forks are produced when DNA denatures at an origin, allowing for bidirectional DNA synthesis. Each fork moves in opposite directions along the DNA strand, with one moving towards the replication fork and the other moving away from it.
Difference between eukaryotic DNA replication and bacterial replication?
Eukaryotic DNA replication is more complex and occurs in the nucleus of the cell, involving multiple origins of replication and coordination with the cell cycle. Bacterial replication is simpler and occurs in the cytoplasm, often with a single origin of replication and a faster rate of replication. Eukaryotic replication also involves telomeres and histones, which are not present in bacterial replication.
What sets of materials are required by both eukaryotes and prokaryotes for replication?
Both eukaryotes and prokaryotes require nucleotides (A, T/U, C, G) for DNA replication, DNA polymerase enzymes for synthesizing new DNA strands, and primers to initiate the replication process. Other materials like ATP for energy and various cofactors and proteins are also necessary for efficient DNA replication.
Why does replication in prokaryotes differs from replication in eukaryotes?
Replication in prokaryotes differs from replication in eukaryotes for prokaryotic chromosomes have a single origin of replication, whereas eukaryotic chromosomes have many. Eukaryotes and prokaryotes for replication double stranded DNA, four kinds of dNTPS, primers, and origins.
Where does the DNA replication take place in prokaryotic cell?
Replication of cellular components such as DNA takes place within the cytoplasm or nucleoid which some prokaryotes have. A nucleoid is a primitive form of a nucleus.
How does DNA replication differ in prokaryotes and eukaryotes?
1. In eukaryotic cells replication forks make several start sites along the DNA strand which forms replication "bubbles" which get larger the more DNA is copied, and stop when DNA replication is complete. In prokaryotic cell's DNA is formed in a loop, two replication forks start along one part of the loop (origin replication) and the replication forks copy DNA in opposite directions until they meet at the other side of the loop, making an exact copy of DNA.
What is it called when DNA runs in opposite directions?
The process is called bidirectional replication. In this process, DNA polymerases synthesize new strands in opposite directions on each template strand.
Do replication forks speed up replication?
Yes, replication forks do speed up the replication process by allowing DNA synthesis to occur simultaneously in both directions around the circular DNA molecule in prokaryotes or at the two replication forks in eukaryotes. This helps to expedite the replication process and minimize the time needed for DNA replication.
What are enzymes that have a proofreading role in DNA replication?
DNA polymerases, such as DNA polymerase III in prokaryotes and DNA polymerase delta in eukaryotes, have proofreading activities during DNA replication. These enzymes possess exonuclease activity, allowing them to detect and correct errors in newly synthesized DNA strands by removing misincorporated nucleotides. This proofreading function helps maintain the fidelity of DNA replication.
Where does the Replication of DNA molecule happens?
It occurs in the nucleus during the S Phase.
Which are characteristics of DNA replication?
DNA replication is semi-conservative, meaning each new DNA molecule contains one strand from the original template and one newly synthesized strand. It occurs through a series of steps including unwinding of the double helix, formation of replication forks, and synthesis of new strands using complementary base pairing. The process is highly accurate due to proofreading mechanisms that help correct errors during replication.
