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What does Active Site mean?
The active site is a specific region on an enzyme where substrates bind and undergo a chemical reaction. It is typically a pocket or groove on the enzyme's surface that accommodates the substrate molecules. The active site plays a crucial role in catalyzing biochemical reactions.
What is the molecule the enzyme works on called?
The molecule that an enzyme acts on is known as a substrate. Enzymes are proteins that catalyze chemical reactions by binding to their specific substrate and facilitating the conversion of the substrate into product(s).
The initial rate of an enzyme catalysed reaction depend on?
Based on Michaelis-Menten enzyme kinetics, the initial rate of reaction, vi, is dependent on maximum rate Vmax, substrate concentration [S], and the enzyme's Michaelis constant Km, which represents the the tendency of the substrate/enzyme complex to dissociate. The dependence on enzyme concentration is factored into the maximum rate. The equation to describe this is: vi = Vmax([S]/(Km+[S])) Follow the link below for details.
What do you know about the consumption of an enzyme during a metabolic reaction in the body?
Enzymes are specialized proteins that facilitate chemical reactions in the body by lowering their activation energy, or the energy required to initiate a reaction. They are never consumed by the reaction themselves; therefore, enzymes can be re-used many times. Enzymes are specific to a particular substrate and usually facilitate the reaction by undergoing a conformational change when encountering the substrate, transforming it into a product or an intermediate.
Why would you expect the rate of an enzyme-catalyzed reaction to increase proportionately to enzyme concentration given an unlimited supply of substrate?
No, since the reaction reaches a max rate depending on the speed of which the Enzyme bonds to the substrate and the speed at which the enzyme catalyzes the reaction to produce enzyme and product (shown below). E + S --> ES (E - enzyme, S - substrate, P - products) ES --> E + P Thus, if each reaction rate is not equal to each other, the rate of the overall reaction is not only proportional to both the concentration of enzyme and substrate.
When the substrates are bound to the enzyme it is called the?
An active site. Sometimes the active site can be disabled from inhibitors.
What are the reactants of enzyme-catalyzed reactions know as?
The reactants of enzyme-catalyzed reactions are known as substrates. Substrates bind to the active site of an enzyme, where the reaction takes place. Enzymes help lower the activation energy required for the reaction to occur.
What is a macromolecule catalyst called?
Biochemical macromolecular 'catalysts' are known as Enzymes. Each one is Throughly Specific in its chemical job(s).
Calculation fraction of enzyme bound to substrate?
The fraction of enzyme bound to substrate can be calculated using the Michaelis-Menten equation: [ES] / [E]t = [S] / (Km + [S]), where [ES] is the concentration of enzyme-substrate complex, [E]t is the total enzyme concentration, [S] is the substrate concentration, and Km is the Michaelis constant. This equation gives the ratio of the concentration of enzyme bound to substrate to the total enzyme concentration at a given substrate concentration.
S aureus positive or negative for gelatinase production?
Staphylococcus aureus is positive for gelatinase production. Gelatinase is an enzyme that hydrolyzes gelatin, and the presence of this enzyme can be demonstrated through specific laboratory tests, such as the gelatin hydrolysis test. This characteristic can help differentiate S. aureus from other staphylococcal species that may not produce gelatinase.
What does Active Site mean?
The active site is a specific region on an enzyme where substrates bind and undergo a chemical reaction. It is typically a pocket or groove on the enzyme's surface that accommodates the substrate molecules. The active site plays a crucial role in catalyzing biochemical reactions.
How do you determine Vmax in enzyme kinetics?
Vmax is the maxim initial velocity (Vo) that an enzyme can achieve. Initial velocity is defined as the catalytic rate when substrate concentration is high, enough to saturate the enzyme, and the product concentration is low enough to neglect the rate of the reverse reaction. Therefore, the Vmax is the maximum catalytic rate that can be achieved by a particular enzyme. Km is determined as the substrate concentration at which 1/2 Vmax is achieved. This kinetic parameter therefore importantly defines the affinity of the substrate for the enzyme. These two parameters for a specific enzyme defines: Vmax - the rate at which a substrate will be converted to product once bound to the enzyme. Km - how effectively the enzyme would bind he substrate, hence affinity.
Who does the substrate work on enzyme action?
The substrate is the molecule on which the enzyme acts. It binds to the active site of the enzyme, leading to catalysis of the chemical reaction. The shape and chemical properties of the substrate are important in determining which enzyme can act on it.
What is the molecule the enzyme works on called?
The molecule that an enzyme acts on is known as a substrate. Enzymes are proteins that catalyze chemical reactions by binding to their specific substrate and facilitating the conversion of the substrate into product(s).
The initial rate of an enzyme catalysed reaction depend on?
Based on Michaelis-Menten enzyme kinetics, the initial rate of reaction, vi, is dependent on maximum rate Vmax, substrate concentration [S], and the enzyme's Michaelis constant Km, which represents the the tendency of the substrate/enzyme complex to dissociate. The dependence on enzyme concentration is factored into the maximum rate. The equation to describe this is: vi = Vmax([S]/(Km+[S])) Follow the link below for details.
What enzyme is not found in S epidermidis but is found in S aureus?
Coagulase is an enzyme that is found in Staphylococcus aureus but not in Staphylococcus epidermidis. It helps S. aureus to form blood clots and evade the host immune response.
What do you know about the consumption of an enzyme during a metabolic reaction in the body?
Enzymes are specialized proteins that facilitate chemical reactions in the body by lowering their activation energy, or the energy required to initiate a reaction. They are never consumed by the reaction themselves; therefore, enzymes can be re-used many times. Enzymes are specific to a particular substrate and usually facilitate the reaction by undergoing a conformational change when encountering the substrate, transforming it into a product or an intermediate.
