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Reasons why a smear from a gram-positive control showed a gram-negative result?
Possible reasons for a gram-positive control smear showing a gram-negative result could include errors in the staining process, contamination of the specimen with gram-negative bacteria, or misidentification of the control as a gram-positive organism when it is actually gram-negative. Additional troubleshooting and repeat testing may be needed to confirm the result.
Why are gram negative eubacteria usually much easier to disrupt by sonic oscillation than gram positive eubacteria?
Gram-negative eubacteria have a thinner peptidoglycan layer in their cell wall compared to gram-positive eubacteria. This makes them more susceptible to disruption by sonic oscillation, as the thinner cell wall offers less structural support and protection. Additionally, gram-negative bacteria have an outer membrane that can be disrupted more easily than the thick peptidoglycan layer of gram-positive bacteria.
Why are gram positive bacteria more heat resistant than gram negative bacteria?
Gram positive bacteria contain more peptidoglycan than gram negative bacteria which is the substance responsible for the increased strength of gram positive bacterias cell walls therefore making them more resistant to heat. Gram negative do contain peptidoglycan but much, much less
How could you differentiate between e coli and staph epidermidis using a simple stain?
E.coli is a gram negative rod mostly isolated from urine while staph is a gram positive cocci. You can differentiate them by color. E. Coli is Pink which is negative while Staph is Purple with is positive.
What does gram positive and gram negative mean?
Gram positive and gram negative is one way of classifying a bacteria.Gram positive bacterias have a cell wall which will stain in violet color. Gram negative bacterias, however, have an extra layer of cell wall around them which prevents the cell wall from being dyed. These gram negative bacterias will need an additional stain called safranin to make the bacterias redish, or pink color. Also Gram positive bacterias have a higher amount of RNA than gram negative baterias. This could be because indirectly peptidoglycan is a protein and the higher amount of protein production means a higher amount of RNA production.there is only difference in gram positive and negative is of cell wall as :The Gram positive cell wallThe Gram positive cell wall is characterised by the presence of a very thick peptidoglycan layer, which is responsible for the retention of the crystal violet dyes during the Gram staining procedure. It is found exclusively in organisms belonging to the Actinobacteria (or high %G+C Gram positive organisms) and the Firmicutes (or low %G+C Gram positive organisms). Bacteria within the Deinococcus-Thermus group may also exhibit Gram positive staining behaviour but contain some cell wall structures typical of Gram negative organisms. Imbedded in the Gram positive cell wall are polyalcohols called teichoic acids, some of which are lipid-linked to form lipoteichoic acids. Because lipoteichoic acids are covalently linked to lipids within the cytoplasmic membrane they are responsible for linking the peptidoglycan to the cytoplasmic membrane. Teichoic acids give the Gram positive cell wall an overall negative charge due to the presence of phosphodiester bonds between teichoic acid monomers.The Gram negative cell wallUnlike the Gram positive cell wall, the Gram negative cell wall contains a thin peptidoglycan layer adjacent to the cytoplasmic membrane, which is responsible for the cell wall's inability to retain the crystal violet stain upon decolourisation with ethanol during Gram staining. In addition to the peptidoglycan layer the Gram negative cell wall also contains an additional outer membrane composed by phospholipids and lipopolysaccharides which face into the external environment. The highly charged nature of lipopolysaccharides confer an overall negative charge to the Gram negative cell wall. The chemical structure of the outer membrane lipopolysaccharides is often unique to specific bacterial strains (i.e. sub-species) and is responsible for many of the antigenic properties of these strains.As a phospholipid bilayer, the lipid portion of the outer membrane is largely impermeable to all charged molecules. However, channels called porins are present in the outer membrane that allow for passive transport of many ions, sugars and amino acids across the outer membrane. These molecules are therefore present in the periplasm, the region between the plasma membrane and outer membrane. The periplasm contains the peptidoglycan layer and many proteins responsible for substrate binding or hydrolysis and reception of extracellular signals. The periplasm is thought to exist as a gel-like state rather than a liquid due to the high concentration of proteins and peptidoglycan found within it. Because of its location between the cytoplasmic and outer membranes, signals received and substrates bound are available to be transported across the cytoplasmic membrane using transport and signalling proteins imbedded there.
What is negative subtracted by a positive?
The Answer could be neither positive nor negative because the positivve could be bigger than the negative or the neagative could be bigger than the positive
How Can you elimintate gram positive bacteria from a gram positive gram negative mixture with mild detergent treatment?
as gram negative have low content of phospholipids than positive detergents at low concentration effectively kill negative bacteria
When you add a positive and a negative what does it become?
the answer could be negative or positive depending on whether the negative number you're adding is bigger than the positive number
Are negative numbers bigger or positive?
Positive numbers are larger than negative ones.
Is a positive number bigger or smaller than a negative number?
It is bigger
Is positive 8 bigger than negative 3?
no
Is a negative integer bigger then a positive integer?
no, all negative numbers are smaller than positive numbers
Is a negative number added by a postitive number a negative number?
sometimes... if the negative number is bigger than a positive number, than the answer is negative. if the positive number is bigger than a negative number, then the answer is positive. if they are the same, then the answer is zero. here is a helpful hint. -x+y is the same as y-x.
Is a negative number bigger than a positive number?
Never.
Is positive 100 bigger than negative 101?
Yes
What is 5minus positive 9?
The answer to this is -4, or negative 4. A positive number minus a positive number bigger than it will always be negative.
Is a decimal bigger than a negative number?
If the decimal is positive, than a negative number is less than 0.
