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Is it true that in a cell fractionation the first step is to place the cells into a centrifuge?
Wiki User
∙ 10y ago
In cell fractionation, the first step is to place the cells into a centrifuge
Wiki User
∙ 10y ago
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How do you isolate a plant cell?
A plant cell can be isolated by using different priscribed techniques keeping in mind the sourse of isolation. For eg. to isolate a leaf cell first:- collect the leaf, homogenase it (crush it) in proper medium then add the mixture in centrifugation tube and centrifuge it repeatedly for 2 to 3 times. discard the supernetant and inoculate the debris in suitable culture media keeping the auxin and cytokinin ratio equal. CALLUS formation take place which is mass of undifferentiated cells. Now from this callus we can isolate plant cells.
Reactions in cells take place at about?
the answer is 40 degrees Celsius for certain
Where does the aerobic process of cellular respiration take place?
Aerobic respiration is an almost universal process - carried out by most animals and plants.It consists of several stages, the first of which is shared with anaerobic respiration and takes place in the cytoplasm of cells. The purely aerobic reactions take place inside mitochondria, small specialized organelles within the cytoplasm of all body cells. More active cells have more mitochondria.
What is use to build and repair body cells?
proteins!!
Were does transcription take place?
in happens in the cytoplasmNo, it doesn't. Translation occurs in the cytoplasm, whereas transcription ( the process in which an exact copy of one strand of DNA is made for the production of proteins) takes place in the nucleus. For details google 'transcription animation'. It should be the first hit.
How the process fractionation takes place?
Distillation
Why do you need a place a second tube into centrifuge?
Balance.
How do you determine mass of an object?
If you are in an atmosphere with a gravitaional pull then you will want to use a balance, if not (like if you were in a place with no gravity) then you want to use a centrifuge.
Where in the cell does the first stage respiration take place?
AnswerIn the cytoplasm of the organism's cells
Who was the first to describe cells?
i think the fist scientist to describe a cell was ROBERT HOOKE because he was the one who wrote about them in the first place.
What is cell fractionation used to study components that make up the cells?
Cell fractionation can be defined as the process of separation of similar and homogeneous sets of organelles from a heterogeneous population of cells. This process can be also used to obtain a part of the whole organelle like the mitochondrion, Deoxyribonucleic acid [DNA], Ribonucleic Acid [RNA], Ribosomes. Soluble proteins specific molecules of compounds can also be obtained by this sophisticated process. There are three prime steps involved in the process of cell fractionation. The steps are: 1. Cell Disruption - Cell disruption refers to the process of liberating the biological and organic molecules which are within a cell. The power needed for the disruption of some cell is quite high - for example E.Coli. It is even higher in other organisms like yeast thus, increasing the cost of disruption and the usage of sophisticated tools and machinery. The tissue is homogenized in a solution in which the water content is maintained equal to the water content within the cell - thus no osmotic damage takes place in the cell. Other methods like mincing, chopping, osmotic shock are used to liberate the cell organelles from the cell. Sometimes, the tissue is kept in ice-cold water or a buffered solution to prevent damages and to prevent any change in the chemical composition in the cell. 2. Filtration - This step involves the filtering out of the macro-sized particles that come along with the cell organelle. This step is not mandatory in some cases. But during the fractionation of Animal tissues, filtration through gauze or a suction filter is necessary in order to leave out the fluid connective tissues as a residue. 3. Purification and Segregation - The filtrate obtained in the previous step is rotated at a very high speed thereby increasing the gravitational force sequentially. The different organelles are segregated according to their density within the test tube. This process is called centrifugation. These three steps account for the whole process of Cell Fractionation.
How do you build a centrifuge?
To build a centrifuge you first need to decide what G force you need to generate. This is a function of the distance that the unit to be centrifuged is from the center axis and the rotational frequency (RPM). A major difficult you might run into is balancing the unit you make. The basic components of a centrifuge are the motor, case, rotor, and shield. The rotor holds the tubes, the case holds the motor in place and is a sturdy base, the shield protects you from any debri that could be thrown from an unbalanced rotor. If you MUST build a centrifuge you will need to learn to machine an accurate and balanced rotor. You are really just better off buying one. If you are in search of a cheap and simple centrifuge for playing with, use some tubing and an egg beater like these students did: http://www.rsc.org/delivery/_ArticleLinking/DisplayHTMLArticleforfree.cfm?JournalCode=LC&Year=2008&ManuscriptID=b809830c&Iss=Advance_Article
What cells does respiration take place in?
Cellular respiration takes place in all living cells , both prokaryotic cells and in eukaryotic cells.
Where in cell does the first stage of respiration take place?
AnswerIn the cytoplasm of the organism's cells
Who was the first scientist first to described cells?
i think the fist scientist to describe a cell was ROBERT HOOKE because he was the one who wrote about them in the first place.
How do you isolate a plant cell?
A plant cell can be isolated by using different priscribed techniques keeping in mind the sourse of isolation. For eg. to isolate a leaf cell first:- collect the leaf, homogenase it (crush it) in proper medium then add the mixture in centrifugation tube and centrifuge it repeatedly for 2 to 3 times. discard the supernetant and inoculate the debris in suitable culture media keeping the auxin and cytokinin ratio equal. CALLUS formation take place which is mass of undifferentiated cells. Now from this callus we can isolate plant cells.
In what cells does photosynthesis take place?
plant cells
