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What is the purpose of the buffer in PCR?
The purpose of the buffer in PCR, I assume you talking about the 5 or 10 times PCR buffer that is provided with enzyme. Buffer is needed to give the correct pH and pottasium ion concentration for the DNA polymerase enzyme (usually DNA polymerase from Thermus aquaticus) to function.
Which of the following characteristics is usually shared by bacteria and fungi and plants?
Cell walls are a characteristic shared by bacteria, fungi, and plants. These structures provide support and protection for the cells.
How are genes turned on and off in a cell?
Each cells in our body expresses. That means turning ON only the part of its genes. The rest of the part are usually repressed (i.e turned off). The process of gene turning ON and OFF is called as gene regulation. And it is one of the important part of each cell development and activities. Because this is the stage where each cell in our body is differentiated from others. For example the cardiac cell is different from nephrotic cells, neurons, muscle cells etc. There are various cell factors and environmental factors that affect the gene regulation. The signals from adjacent or other cells activates gene regulation. Gene regulation can happen at any moment during gene expression but usually occurs at the transcription phase (i.e Transforming the DNA into mRNA [Messenger RNA]).
In which reaction mixture did hydrolysis of starch occur?
The hydrolysis of starch occurs in the reaction mixture containing the enzyme amylase, which breaks down starch into smaller sugars such as maltose and glucose. This process of breaking down starch into simpler sugars is known as enzymatic hydrolysis.
What is the affect of temperature on cellular respiration?
Usually, the higher the temperature the faster enzymes react and the quicker a reaction moves forward, however, if the temperature gets too hot, it can denature the proteins involved in the reaction.
What is the general features of allosteric regulation using the feedback inhibition?
enzymes situated at key steps in metabolic pathways are modulated by allosteric effectors these effectors are usually produced elsewhere in the pathway effectors may be feed-forward activators or feedback inhibitors kinetics are sigmoid ("S-shaped")
What is metabolic regulation?
Intrinsic regulation is when the products of the reaction regulate the rate of the reaction, acting as regulators (usually allosteric) . i.e it is self-limiting. Pyruvate + CoASH + NAD+ --> Acetyl-CoA + NADH2 + CO2 This is an example of the bridging reaction wherein pyruvate reacts with CoA and NAD+ to form Acetyl-Coa. The intrinsic regulator here is Acetyl-Coa as if it increases in concentration it inhibits the reaction (following Le Chateliers principle). NADH2 is also an INTRINSIC regulator here.
An authoritative decree or direction usually set forth by a municipal regulation?
ordinance
What are the dimensions of a college-sized pool?
Usually regulation Olympic size. approx. 50feet
An authoratative devree or direction usually set forth by a municipal regulation is called an?
injunction
An authoritative decree or direction usually set forth by a municipal regulation is called?
Ordinance
Which one of the following is defined as gives further information as to how a statute must be followed usually addressing a specific goal or objective?
That would be "administrative regulation", which provides specific guidelines on how a statute should be implemented to achieve its intended goal or objective.
Difference between allosteric and covalent modulation?
The 2 mechanisms to alter protein shape are allosteric and covalent modulation. Allosteric: If the protein contains 2 binding sites, the noncovalent binding of a ligand to one site can alter the shape & characteristics of the other site. -One binding site on an allosteric protein, the functional site, carries out the proteins physiological functions. -The other binding site is the regulatory site, and the ligand that binds to it is called the modulator molecule because it changes the shape! Covalent: Covalent bonding of charged chemical groups to some of the proteins side chains changes the shape and characteristics of the protein. Usually a phosphate group is covalently bonded, in the reaction called phosphorylation.
What is line in line regulation?
Line refers to the a.c. supply line. Line regulation is the change in output of a device, usually a power supply, which is due to variations in the voltage of the a.c. supply.
A regulation sign is usually what color?
They are often white, although some are red (stop and yield signs).
What color and shape are pavement-width transition signs?
Regulation signs are usually white rectangles with black lettering.
What is the recovery period following the injection of botulinum toxin?
There is usually no recovery period following the injection of botulinum toxin. The maximal effects are usually seen four to seven days following injection.
