DNA ligase
sticky ends
the enzymes cause sticky ends to form------------------------------------------------the question is WHAT ARE STICKY ENDS, not how are sticky ends formed.the answer is "single stranded pieces of DNA left at the ends of restriction fragmants"-simone :)The actual answer is: single-stranded ends of fragments of double-stranded DNA
HaeIII
These sticky ends, if they two pieces match, they will join together to form a recombinant DNA.
A butterflies feet are sticky because they use them to taste. A butterfly uses its feet to taste the nectar of flowers and nectar is a sticky substance.
sticky ends
sticky ends are more specific and easier to ligate
Sticky ends are produced by cutting the DNA in a staggered manner within the recognition site producing single-stranded DNA ends. These ends have identical nucleotide sequence and are sticky because they can hydrogen-bond to complementary tails of other DNA fragments cut by the same restriction enzyme.
the enzymes cause sticky ends to form------------------------------------------------the question is WHAT ARE STICKY ENDS, not how are sticky ends formed.the answer is "single stranded pieces of DNA left at the ends of restriction fragmants"-simone :)The actual answer is: single-stranded ends of fragments of double-stranded DNA
A Sticky End, referring to Biology is recombinant DNA. After DNA has been cut by a restriction enzyme it has "sticky ends" or recombinant DNA at the ends.
They are called "sticky ends".
Generally, cDNA's do not have sticky ends - also known as overhangs. However, if the research requires it, they can be engineered to have overhangs
HaeIII
These sticky ends, if they two pieces match, they will join together to form a recombinant DNA.
Restriction Enzyme
Sticky tape
can join with each other.