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What are the steps of using recombinant DNA technology?
1 Isolate DNA 2 Cut DNA with a restriction enzyme 3 Mix the DNA's and join then together by using DNA ligase 4 Insert the recombinant plasmid into a host bacterium 5 Allow the bacterium to reproduce
What end of a growing strand are DNA nucleotides added to?
5' end (nucleotides are added from 3' toward 5')
If you were to stretch all the DNA in all our cells end to end from the earth to the sun how many rounds can we go?
we can go about 5 times from the earth to to sun
What type of mechanical wave is produced by pushing sharply on the end of a spring toy?
Buoyancy
Why primers are phosphorylated in 5' end?
Primers are phosphorylated at the 5' end to facilitate the initiation of DNA synthesis during PCR and other molecular biology techniques. The phosphate group is necessary for the attachment of the primer to the DNA polymerase enzyme, allowing it to extend the primer by adding nucleotides. Additionally, phosphorylation helps ensure that the primers can be efficiently ligated or incorporated into the DNA strand, enhancing the overall efficiency of the amplification process.
What can restricted enzymes leave?
Restriction enzymes can leave blunt or sticky ends. This will depend on where they cut the DNA, if they cut at the same point on both strands, they will leave a blunt end, if they cut at different points, they will leave sticky ends. For example: (| represents point of cut) Blunt end ATC|GCTA TAG|CGAT Sticky end A|TCGCTA TAGCGA|T
Diagram the difference between a blunt cut and a sticky end.?
A blunt cut refers to the clean, straight cut made across DNA strands, resulting in two ends that are smooth and even, allowing for straightforward ligation. In contrast, a sticky end is created by an enzyme that cuts the DNA in a staggered manner, leaving overhanging sequences (or "sticky" ends) that can easily base-pair with complementary sequences of other DNA fragments. This difference in structure allows sticky ends to facilitate more efficient and specific DNA recombination compared to blunt ends, which require more precise alignment for ligation.
Which enzymes does not make sticky ends?
Enzymes that do not create sticky ends include blunt-end cutting enzymes such as SmaI and PvuII. These enzymes produce blunt-ended DNA molecules with no overhangs, making them unsuitable for creating cohesive ends for ligation.
What is a sticky end?
A Sticky End, referring to Biology is recombinant DNA. After DNA has been cut by a restriction enzyme it has "sticky ends" or recombinant DNA at the ends.
What are the types of cloning?
In general, sticky end cloning and blunt end cloning
How can a restriction enzyme leave sticky ends between DNA fragments?
The sticky ends generated by restriction enzymes can easily be joined using an enzyme called ligase. Blunt ends however, cannot be joined so easily. This is why restiction enzymes that create sticky ends are more useful. If blunt ends result, small segments called modifiers are attached to the sticky ends. These modifiers are nucleotide sequences that have sticky ends and attach to the blunt ends, thus making them sticky ends.
List the two nucleotide sequence that are complementary to the sticky end sequence on the human DNA?
The complementary nucleotide sequence to a sticky end sequence on human DNA would be its reverse complement sequence. For example, if the sticky end sequence is "AATT", its complementary sequence would be "TTAA".
What are steps involved in cohesive and blunt end ligation?
For cohesive end ligation, the steps involve digestion of the vector and insert DNA with compatible restriction enzymes, followed by purification of the digested DNA fragments, mix them together in the presence of ligase enzyme, and transform the ligated DNA into a host organism. For blunt end ligation, the steps are similar with the exception that the DNA fragments are generated by restriction enzymes that produce blunt ends, so there is no need to worry about complementary overhangs.
A dna fragment with a sticky end that reads -attcg will bind with another dna fragment with a sticky end that reads?
ATTCG signify adenine (A), thymine (T), cytosine (C) and guanine (G). The bonding pairs are AT, GC when DNA replicates. Therefore the fragment ATTCG will bind to TAAGC.
If the sticky end of a nucleotide sequence is TTAA what is next?
If the sticky end of a sequence is TTAA, it can bind to a DNA molecule with the sequence AATT
What are stick ends?
the enzymes cause sticky ends to form------------------------------------------------the question is WHAT ARE STICKY ENDS, not how are sticky ends formed.the answer is "single stranded pieces of DNA left at the ends of restriction fragmants"-simone :)The actual answer is: single-stranded ends of fragments of double-stranded DNA
What does blunt means in angle?
blunt means dull or rounded, for example: "A knife has a sharp end and a blunt end ."
